scholarly journals Screening and characterization of anti‐SEB peptides using a bacterial display library and microfluidic magnetic sorting

2014 ◽  
Vol 27 (12) ◽  
pp. 739-745 ◽  
Author(s):  
Joshua M. Kogot ◽  
Joseph M. Pennington ◽  
Deborah A. Sarkes ◽  
David A. Kingery ◽  
Paul M. Pellegrino ◽  
...  
Keyword(s):  
Bacterial Display ◽  
Magnetic Sorting ◽  
Bacterial Display Library

scholarly journals High-resolution epitope mapping and characterization of SARS-CoV-2 antibodies in large cohorts of subjects with COVID-19

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Winston A. Haynes ◽  
Kathy Kamath ◽  
Joel Bozekowski ◽  
Elisabeth Baum-Jones ◽  
Melissa Campbell ◽  
...  
Keyword(s):  
Epitope Mapping ◽  
Severe Disease ◽  
Neutralization Activity ◽  
Bacterial Display ◽  
Repertoire Analysis ◽  
Bacterial Display Library ◽  
Dominant Epitope ◽  
Epitope Binding ◽  
Antibody Epitopes

AbstractAs Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) continues to spread, characterization of its antibody epitopes, emerging strains, related coronaviruses, and even the human proteome in naturally infected patients can guide the development of effective vaccines and therapies. Since traditional epitope identification tools are dependent upon pre-defined peptide sequences, they are not readily adaptable to diverse viral proteomes. The Serum Epitope Repertoire Analysis (SERA) platform leverages a high diversity random bacterial display library to identify proteome-independent epitope binding specificities which are then analyzed in the context of organisms of interest. When evaluating immune response in the context of SARS-CoV-2, we identify dominant epitope regions and motifs which demonstrate potential to classify mild from severe disease and relate to neutralization activity. We highlight SARS-CoV-2 epitopes that are cross-reactive with other coronaviruses and demonstrate decreased epitope signal for mutant SARS-CoV-2 strains. Collectively, the evolution of SARS-CoV-2 mutants towards reduced antibody response highlight the importance of data-driven development of the vaccines and therapies to treat COVID-19.

A Neutralization scFv Antibody against IL-1β Isolated from a NIPA-based Bacterial Display Library

2013 ◽  
Vol 14 (6) ◽  
pp. 571-581 ◽  
Author(s):  
Tianhe Li ◽  
Liming Xu ◽  
Guiping Ren ◽  
Chengkai Yin ◽  
Bing Zhou ◽  
...  
Keyword(s):  
Scfv Antibody ◽  
Bacterial Display ◽  
Bacterial Display Library

scholarly journals Characterization of an apical ceramide-enriched compartment regulating ciliogenesis

2012 ◽  
Vol 23 (16) ◽  
pp. 3156-3166 ◽  
Author(s):  
Qian He ◽  
Guanghu Wang ◽  
Somsankar Dasgupta ◽  
Michael Dinkins ◽  
Gu Zhu ◽  
...  
Keyword(s):  
Protein Complex ◽  
Primary Cilia ◽  
Mdck Cells ◽  
Trichostatin A ◽  
Acid Sphingomyelinase ◽  
Dominant Negative ◽  
Dominant Negative Mutant ◽  
Tubulin Acetylation ◽  
Magnetic Sorting

We show that in Madin–Darby canine kidney (MDCK) cells, an apical ceramide-enriched compartment (ACEC) at the base of primary cilia is colocalized with Rab11a. Ceramide and Rab11a vesicles isolated by magnetic sorting contain a highly similar profile of proteins (atypical protein kinase C [aPKC], Cdc42, Sec8, Rab11a, and Rab8) and ceramide species, suggesting the presence of a ciliogenic protein complex associated with ceramide at the ACEC. It is intriguing that C16 and C18 ceramide, although less abundant ceramide species in MDCK cells, are highly enriched in ceramide and Rab11a vesicles. Expression of a ceramide-binding but dominant-negative mutant of aPKC suppresses ciliogenesis, indicating that the association of ceramide with aPKC is critical for the formation of this complex. Our results indicate that ciliogenic ceramide is derived from apical sphingomyelin (SM) that is endocytosed and then converted to the ACEC. Consistently, inhibition of acid sphingomyelinase with imipramine disrupts ACEC formation, association of ciliogenic proteins with Rab11a vesicles, and cilium formation. Ciliogenesis is rescued by the histone deacetylase (HDAC) inhibitor trichostatin A, indicating that ceramide promotes tubulin acetylation in cilia. Taken together, our results suggest that the ACEC is a novel compartment in which SM-derived ceramide induces formation of a ciliogenic lipid–protein complex that sustains primary cilia by preventing deacetylation of microtubules.

Isolation and characterization of anti-SEB peptides using magnetic sorting and bacterial peptide display library technology

2012 ◽  
Author(s):  
Joseph M. Pennington ◽  
Joshua M. Kogot ◽  
Deborah A. Sarkes ◽  
Paul M. Pellegrino ◽  
Dimitra N. Stratis-Cullum
Keyword(s):  
Isolation And Characterization ◽  
Magnetic Sorting ◽  
Peptide Display ◽  
Library Technology ◽  
Peptide Display Library

Detection, Isolation, and Characterization of α-fetoprotein-specific T Cell Populations and Clones Using MHC Class I Multimer Magnetic Sorting

2008 ◽  
Vol 31 (3) ◽  
pp. 246-253 ◽  
Author(s):  
Virginie Pichard ◽  
Pierre Joseph Royer ◽  
Carine Richou ◽  
Estelle Cauchin ◽  
Karen Goebes ◽  
...  
Keyword(s):  
T Cell ◽  
Mhc Class I ◽  
Class I ◽  
Cell Populations ◽  
Isolation And Characterization ◽  
Magnetic Sorting

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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