Microtubule-dependent changes in morphology and localization of chloride transport proteins in gill mitochondria-rich cells of the tilapia,Oreochromis mossambicus

2016 ◽  
Vol 277 (8) ◽  
pp. 1113-1122
Author(s):  
Wen-Kai Yang ◽  
Yu-Ching Wu ◽  
Cheng-Hao Tang ◽  
Tsung-Han Lee
Keyword(s):  
Chloride Transport ◽  
Oreochromis Mossambicus ◽  
Transport Proteins ◽  
Gill Mitochondria

scholarly journals Expression and Localization of CLC Chloride Transport Proteins in the Avian Retina

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17647 ◽  
Author(s):  
Emily McMains ◽  
Vijai Krishnan ◽  
Sujitha Prasad ◽  
Evanna Gleason
Keyword(s):  
Chloride Transport ◽  
Transport Proteins ◽  
Avian Retina

Role of chloride transport proteins in the vasorelaxant action of nitroprusside in isolated rat aorta

2006 ◽  
Vol 553 (1-3) ◽  
pp. 205-208 ◽  
Author(s):  
Marta Valero ◽  
Désirée Pereboom ◽  
Ricardo P. Garay ◽  
José Octavio Alda
Keyword(s):  
Chloride Transport ◽  
Rat Aorta ◽  
Transport Proteins ◽  
Isolated Rat

Isoform expression of Na+-K+-ATPase α-subunit in gills of the teleostOreochromis mossambicus

1998 ◽  
Vol 275 (3) ◽  
pp. R926-R932 ◽  
Author(s):  
Tsung-Han Lee ◽  
Jong-Chang Tsai ◽  
Mei-Jane Fang ◽  
Ming-Jiun Yu ◽  
Pung-Pung Hwang
Keyword(s):  
Image Analysis ◽  
Sodium Pump ◽  
Gill Tissue ◽  
Oreochromis Mossambicus ◽  
Acid Base ◽  
Α Subunit ◽  
Specific Antibodies ◽  
Gill Mitochondria ◽  
Isoform Expression ◽  
Α Subunits

Three isoform-specific antibodies, 6F against the α1-isoform of the avian sodium pump, HERED against the rat α2-isoform, and Ax2 against the rat α3-isoform, were used to detect the expression of Na+-K+-ATPase α-subunits in gills of a teleost, the tilapia ( Oreochromis mossambicus). Tilapia gill tissue showed positive reactions to antibodies specific for α1- and α3-isoforms. The results of immunoblots were converted to numerical values (relative intensities) by image analysis for comparisons. Relative amounts of α1-like isoform alone and consequently the ratio of α1-like to α3-like isoforms were higher in gills of seawater-adapted tilapia than in those of freshwater-adapted ones, indicating that the two isoforms respond differently to environmental salinities. In the subsequent immunocytochemical experiments, gill mitochondria-rich cells were demonstrated to immunoreact with antibodies specific for α1- and α3-isoforms. α1-like and α3-like isoforms of gill Na+-K+-ATPase are suggested to be involved in the ion- and osmoregulation mechanisms in tilapia. Moreover, differential expressions of two isoforms may be associated with different functions, secretion and uptake of ions and acid-base regulation, in gills of seawater- and freshwater-adapted tilapia.

Chloride transport in mitochondrion-rich cells of euryhaline tilapia (Oreochromis mossambicus) larvae

2009 ◽  
Vol 297 (4) ◽  
pp. C845-C854 ◽  
Author(s):  
Jiun-Lin Horng ◽  
Pung-Pung Hwang ◽  
Tin-Han Shih ◽  
Zhi-Hong Wen ◽  
Chan-Shing Lin ◽  
...  
Keyword(s):  
Chloride Transport ◽  
Oreochromis Mossambicus ◽  
Skin Surface ◽  
Convincing Evidence ◽  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Exterior Surface ◽  
Electrode Technique ◽  
Apical Opening

A noninvasive scanning ion-selective electrode technique (SIET) was applied to measure Cl− transport at individual mitochondrion-rich cells (MRCs) in the skin of euryhaline tilapia ( Oreochromis mossambicus) larvae. In seawater (SW)-acclimated larvae, outward Cl− gradients (20∼80 mM higher than the background) were measured at the surface, indicating a secretion of Cl− from the skin. By serial probing over the surface of MRCs and adjacent keratinocytes (KCs), a significant outward flux of Cl− was detected at the apical opening (membrane) of MRCs. Treatment with 100 μM ouabain or bumetanide inhibited the Cl− secretion by ∼75%. In freshwater (FW)-acclimated larvae, a lower level of outward Cl− gradients (0.2∼1 mM) was measured at the skin surface. Low-Cl− water (<0.005 mM) acclimation increased the apical Na+-Cl− cotransporter (NCC) immunoreactivity of MRCs in the larval skin. An inward flux of Cl− was detected when probing the exterior surface of a group of MRCs (convex-MRCs) that express the NCC. An NCC inhibitor (100 μM metolazone) reduced the flux by ∼90%. This study provides direct and convincing evidence for Cl− transport by MRCs of SW- and FW-acclimated euryhaline tilapia and the involvement of an apical NCC in Cl− uptake of MRCs of FW-acclimated fish.

scholarly journals Twenty-five years of CLC chloride transport proteins

2015 ◽  
Vol 593 (18) ◽  
pp. 4083-4084 ◽  
Author(s):  
Peying Fong
Keyword(s):  
Chloride Transport ◽  
Transport Proteins

scholarly journals Novel Roles for Chloride Channels, Exchangers, and Regulators in Chronic Inflammatory Airway Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Monica Sala-Rabanal ◽  
Zeynep Yurtsever ◽  
Kayla N. Berry ◽  
Tom J. Brett
Keyword(s):  
Chloride Channel ◽  
Chloride Channels ◽  
Mucociliary Clearance ◽  
Chloride Transport ◽  
Transport Proteins ◽  
Innate Immune ◽  
Mucus Secretion ◽  
Airway Constriction ◽  
Airway Diseases

Chloride transport proteins play critical roles in inflammatory airway diseases, contributing to the detrimental aspects of mucus overproduction, mucus secretion, and airway constriction. However, they also play crucial roles in contributing to the innate immune properties of mucus and mucociliary clearance. In this review, we focus on the emerging novel roles for a chloride channel regulator (CLCA1), a calcium-activated chloride channel (TMEM16A), and two chloride exchangers (SLC26A4/pendrin and SLC26A9) in chronic inflammatory airway diseases.

scholarly journals Distinct Neuropathologic Phenotypes After Disrupting the Chloride Transport Proteins ClC-6 or ClC-7/Ostm1

2010 ◽  
Vol 69 (12) ◽  
pp. 1228-1246 ◽  
Author(s):  
Sarah N.R. Pressey ◽  
Kieran J. O'Donnell ◽  
Tobias Stauber ◽  
Jens C. Fuhrmann ◽  
Jaana Tyynelä ◽  
...  
Keyword(s):  
Chloride Transport ◽  
Transport Proteins
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