A correlated light and electron microscopic study of the structure and secretory activity of the accessory salivary glands of the marine gastropods,Conus flavidus andC. vexillum (neogastropoda, conacea)

1983 ◽  
Vol 176 (1) ◽  
pp. 89-111 ◽  
Author(s):  
Michael C. Schultz
Keyword(s):  
Salivary Glands ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Secretory Activity ◽  
Electron Microscopic ◽  
Marine Gastropods

Fine structural evidence for the penetration of the Lyme disease spirochete Borrelia burgdorferi through the gut and salivary tissues of Ixodes dammini

1989 ◽  
Vol 67 (7) ◽  
pp. 1737-1748 ◽  
Author(s):  
J. L. Zung ◽  
S. Lewengrub ◽  
M. A. Rudzinska ◽  
A. Spielman ◽  
S. R. Telford ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Salivary Glands ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Strong Evidence ◽  
Geographic Origin ◽  
Transovarial Transmission ◽  
Electron Microscopic ◽  
The Subject

The route followed by the Lyme disease spirochete Borrelia burgdorferi as it passes from vector to host has been the subject of controversy over whether the spirochete is transmitted through the saliva of the tick or through regurgitation during feeding. In the event that the spirochete's presence in the salivary tissues is transient we employed a detailed electron microscopic study spanning the period of nymphal attachment to the host to determine whether B. burgdorferi invades the salivary acini and ducts. In addition we examined other tissues of the tick to determine the route and mode of migration. Two different groups of nymphs were used in this study. After feeding, spirochetes were found in both groups in the gut lumen, epithelium, and within the salivary glands and ducts. Borrelia is able to pass both inter- and intra-cellularly through these tissues. In one group of unfed nymphs Borrelia was limited to the gut lumen, whereas the second group demonstrated a disseminated infection. This difference might be due to transovarial transmission, the geographic origin, and (or) age of the ticks. The finding of Borrelia within the salivary glands and ducts provides strong evidence for the salivary transmission of the Lyme disease spirochete.

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

1976 ◽  
Vol 34 ◽  
pp. 362-363
Author(s):  
L.A. Dell
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Plane Layer ◽  
Ultrastructural Level ◽  
Spinal Fluid ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Easy Method ◽  
Cell Pellet ◽  
A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

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