Effects of noradrenaline administration on the interrenal gland of the newt,Triturus carnifex: Evidence of intra-adrenal paracrine interactions

2003 ◽  
Vol 259 (1) ◽  
pp. 33-40 ◽  
Author(s):  
A. Capaldo ◽  
F. Gay ◽  
S. Valiante ◽  
V. Laforgia ◽  
L. Varano
Cell Calcium ◽  
1987 ◽  
Vol 8 (4) ◽  
pp. 269-282 ◽  
Author(s):  
I. Lihrmann ◽  
C. Delarue ◽  
F. Homo-Delarche ◽  
M. Feuilloley ◽  
A. Bélanger ◽  
...  

1986 ◽  
Vol 32 (1) ◽  
pp. 127-131 ◽  
Author(s):  
I. Lihrmann ◽  
C. Delarue ◽  
F. Homo-Delarche ◽  
P. Netchitailo ◽  
F. Leboulenger ◽  
...  

1990 ◽  
Vol 5 (1) ◽  
pp. 55-60 ◽  
Author(s):  
L. B. O'Toole ◽  
K.J. Armour ◽  
C. Decourt ◽  
N. Hazon ◽  
B. Lahlou ◽  
...  

ABSTRACT An isolated in-vitro perifused interrenal gland preparation from the dogfish Scyliorhinus canicula was used to study production of quantitatively the major corticosteroid 1α-hydroxycorticosterone (1α-OH-B), measured by radioimmunoassay. Basal secretory rates were 877·1 ± 145 (s.e.m.) fmol/mg per 15 min (n=14) and the preparation remained viable for up to 22 h, as reflected in a brisk response to 10 μm cyclic AMP (cAMP) after this time. Steroid production responded in a dose-dependent manner to porcine ACTH, with 10 μm producing a maximum stimulation of 225% above the basal secretory rate. cAMP (10 μm) produced an increase of 278% above basal, while 1 μm forskolin increased basal secretory rates by 127%. [Val5]- and [Ile5]-angiotensin II (0·1 μm) increased 1α-OH-B production by 120 and 372% respectively over basal secretory rates. Increasing the concentration of K+ in the perfusate from 8 mm to 12, 18, 28 and 40 mm produced a significant rise only at 28 mm. Alterations in the concentration of Na+ and osmolarity of the perifusion medium had inconsistent effects on steroid production. Increased concentrations of urea (from 360 to 720 mm) increased the basal secretory rate by 121%, whilst reducing the concentration of urea (from 360 to 90 mm) had no effect.


1976 ◽  
Vol 54 (4) ◽  
pp. 477-484 ◽  
Author(s):  
H. B. Geiger ◽  
S. H. Song ◽  
A. C. Groom

Isolated, denervated, cat spleens were perfused at constant flow with modified Ringer solution. Perfusion pressure, outflow rate, and outflow red cell concentration were measured against time. After splenic perfusion by 500 ml solution the cell washout curve became a single exponential function, indicating that only cells from the most slowly exchanging red cell compartment remained (these are immature and abnormal cells which adhere to the fine structures of the red pulp). Splenic contraction was induced by injection of 5 μg noradrenaline into the inflow after perfusion by 600 and 1000 ml of fluid, respectively; outflow cell concentration rose 17-fold before returning to baseline value and 32% of red cells in the spleen were expelled. The time course of changes in cell concentration was similar in shape but delayed with respect to that of outflow rate. The transit time of the cells from the site of release to the splenic vein must have exceeded 40 s, which is consistent only with release from the red pulp. Furthermore, at the peak of the cell concentration curve the mean reticulocyte count was 37.8%. Thus immature and abnormal red cells, which comprise the slowly-exchanging compartment, are indeed released from the spleen during contraction.


2004 ◽  
Vol 261 (1) ◽  
pp. 18-25 ◽  
Author(s):  
A. Capaldo ◽  
F. Gay ◽  
S. Valiante ◽  
V. Laforgia ◽  
L. Varano

2018 ◽  
Vol 35 (9) ◽  
pp. 641-649 ◽  
Author(s):  
Marc A. Furrer ◽  
Marc P. Schneider ◽  
Lukas M. Löffel ◽  
Fiona C. Burkhard ◽  
Patrick Y. Wuethrich

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