Constitutive Expression of Interleukin-7 mRNA and Production of IL-7 by a Cloned Murine Thymic Stromal Cell Line

1990 ◽  
Vol 48 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Tsuneaki Sakata ◽  
Shoji Iwagami ◽  
Yuji Tsuruta ◽  
Hiroshi Teraoka ◽  
Yoichi Tatsumi ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Constitutive Expression ◽  
Interleukin 7 ◽  
Stromal Cell Line ◽  
Thymic Stromal Cell

scholarly journals Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

2000 ◽  
Vol 192 (5) ◽  
pp. 671-680 ◽  
Author(s):  
John E. Sims ◽  
Douglas E. Williams ◽  
Philip J. Morrissey ◽  
Kirsten Garka ◽  
Diane Foxworthe ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Line ◽  
Stromal Cell ◽  
Biological Characterization ◽  
Gene Encoding ◽  
Interleukin 7 ◽  
Stromal Cell Line ◽  
Thymic Stromal Cell

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220+BP-1+ pre-B cells.

Morphological analysis of the cellular interaction between thymocytes and a thymic stromal cell line (TEL-2)

1991 ◽  
Vol 230 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Kazuho Hirata ◽  
Keiko Mori ◽  
Keiichiro Nakamura ◽  
Masaru Kawabuchi ◽  
Takeshi Watanabe
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Morphological Analysis ◽  
Cellular Interaction ◽  
Stromal Cell Line ◽  
Thymic Stromal Cell

A murine thymic stromal cell line which may support the differentiation of CD4−8− thymocytes into CD4+8− αβ T cell receptor positive T cells

1992 ◽  
Vol 142 (2) ◽  
pp. 385-397 ◽  
Author(s):  
Yoshihiro Watanabe ◽  
Osam Mazda ◽  
Yu-Ichi Aiba ◽  
Kazuhiro Iwai ◽  
Jun-Ichiro Gyotoku ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Line ◽  
T Cell Receptor ◽  
Stromal Cell ◽  
Cell Receptor ◽  
Stromal Cell Line ◽  
Thymic Stromal Cell

scholarly journals The thymic stromal cell line MTSC4 induced thymocyte apoptosis in a non-MHC-restricted manner

Cell Research ◽  
2004 ◽  
Vol 14 (2) ◽  
pp. 125-133
Author(s):  
Xue Ying HE ◽  
Juan LI ◽  
Xiao Ping QIAN ◽  
Wen Xian FU ◽  
Yan LI ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Stromal Cell Line ◽  
Thymocyte Apoptosis ◽  
Thymic Stromal Cell

scholarly journals Differential roles of stromal cells, interleukin-7, and kit-ligand in the regulation of B lymphopoiesis

Blood ◽  
1992 ◽  
Vol 79 (5) ◽  
pp. 1185-1192 ◽  
Author(s):  
LG Billips ◽  
D Petitte ◽  
K Dorshkind ◽  
R Narayanan ◽  
CP Chiu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Kit Ligand ◽  
Interleukin 7 ◽  
Stromal Cell Line ◽  
Differentiation And Proliferation

Abstract Newly formed B lymphocytes are a population of rapidly renewed cells in the bone marrow of mammals and their steady state production presumably depends on a cascade of regulatory cells and cytokines. Although considerable information has been forthcoming about the role of interleukin-7 (IL-7) in potentiating pre-B-cell proliferation, few studies have addressed the possibility that multiple cytokines are involved in the progression of early events in cellular differentiation and proliferation in this hematopoietic lineage. Our laboratory previously described pre-B-cell differentiation mediated by the bone marrow stromal cell line S17. In this study, we further delineate the role of stromal cells in differentiation and proliferation of pre-B cells. These experiments show that the stromal cell line S17 potentiates the proliferative effect of IL-7 on B-lineage cells and that this S17-derived potentiator can be replaced with recombinant kit- ligand (KL). Our results further show that pre-B-cell formation from B220-, Ig- progenitor cells and expression of mu heavy chain of immunoglobulin is uniquely dependent on the presence of S17 stromal cells and cannot be reproduced with IL-7, KL, or costimulation with both IL-7 and KL. These data contribute to a rapidly evolving model of stromal cell regulation of B-cell production in the marrow and suggest unique roles for IL-7, KL, and as yet uncharacterized stromal cell- derived lymphokines in this process.

scholarly journals Differential roles of stromal cells, interleukin-7, and kit-ligand in the regulation of B lymphopoiesis

Blood ◽  
1992 ◽  
Vol 79 (5) ◽  
pp. 1185-1192 ◽  
Author(s):  
LG Billips ◽  
D Petitte ◽  
K Dorshkind ◽  
R Narayanan ◽  
CP Chiu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cell ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Kit Ligand ◽  
Interleukin 7 ◽  
Stromal Cell Line ◽  
Differentiation And Proliferation

Newly formed B lymphocytes are a population of rapidly renewed cells in the bone marrow of mammals and their steady state production presumably depends on a cascade of regulatory cells and cytokines. Although considerable information has been forthcoming about the role of interleukin-7 (IL-7) in potentiating pre-B-cell proliferation, few studies have addressed the possibility that multiple cytokines are involved in the progression of early events in cellular differentiation and proliferation in this hematopoietic lineage. Our laboratory previously described pre-B-cell differentiation mediated by the bone marrow stromal cell line S17. In this study, we further delineate the role of stromal cells in differentiation and proliferation of pre-B cells. These experiments show that the stromal cell line S17 potentiates the proliferative effect of IL-7 on B-lineage cells and that this S17-derived potentiator can be replaced with recombinant kit- ligand (KL). Our results further show that pre-B-cell formation from B220-, Ig- progenitor cells and expression of mu heavy chain of immunoglobulin is uniquely dependent on the presence of S17 stromal cells and cannot be reproduced with IL-7, KL, or costimulation with both IL-7 and KL. These data contribute to a rapidly evolving model of stromal cell regulation of B-cell production in the marrow and suggest unique roles for IL-7, KL, and as yet uncharacterized stromal cell- derived lymphokines in this process.

Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation.

Endocrinology ◽  
1995 ◽  
Vol 136 (4) ◽  
pp. 1441-1449 ◽  
Author(s):  
S Takahashi ◽  
S V Reddy ◽  
M Dallas ◽  
R Devlin ◽  
J Y Chou ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Cell Formation ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line
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