A mouse thymic stromal cell line producing macrophage-colony stimulating factor and interleukin-6

2000 ◽  
Vol 23 (3) ◽  
pp. 252-256 ◽  
Author(s):  
Chong-Kil Lee ◽  
Jeong Ki Kim ◽  
Kyungjae Kim ◽  
Seong Sun Han
Keyword(s):  
Cell Line ◽  
Interleukin 6 ◽  
Stromal Cell ◽  
Colony Stimulating Factor ◽  
Stromal Cell Line ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor ◽  
Thymic Stromal Cell

scholarly journals Identification of a growth factor for primary murine stroma as macrophage colony-stimulating factor

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2568-2578 ◽  
Author(s):  
EI Deryugina ◽  
BI Ratnikov ◽  
MA Bourdon ◽  
GL Gilmore ◽  
RK Shadduck ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Line ◽  
Stromal Cell ◽  
Gel Filtration ◽  
Colony Stimulating Factor ◽  
Stromal Microenvironment ◽  
Stromal Cell Line ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

Abstract Knowledge of the stromal microenvironment is crucial for understanding the hematopoietic system. We took advantage of an assay that permits analysis of primary stroma-initiating cells (SICs) on the clonal level, and further characterized SICs and the factors that regulate SICs. Stroma formation in this assay is dependent on a high-molecular-weight factor secreted by the stromal cell line AC3.U. Here we show that this factor is identical to macrophage colony-stimulating factor (M-CSF), and that purified M-CSF is sufficient for induction of stroma formation. M-CSF, isolated from the line AC3.U, as well as from L929 cells and COS cells transfected with an expression vector encoding M-CSF, migrated in two peaks as 160- and 650-kD species after gel filtration. These molecular-weight species encompassed all stroma-inducing activity, and both stimulated macrophage colony formation. Affinity chromatography and blocking studies with antibodies specific for M-CSF and c-fms confirmed M-CSF as the sole factor in the supernatant of the stromal cell line AC3.U that promotes stroma formation. Culture of marrow, for as little as 1 week, depleted M-CSF-dependent SIC while increasing the incidence of replatable, factor-independent SIC. This suggests that culture changes the properties of SICs, perhaps by inducing differentiation into mature stromal cells. Thus, our results show a novel function of M-CSF as an important modulator of stroma formation.

scholarly journals Identification of a growth factor for primary murine stroma as macrophage colony-stimulating factor

Blood ◽  
1995 ◽  
Vol 86 (7) ◽  
pp. 2568-2578
Author(s):  
EI Deryugina ◽  
BI Ratnikov ◽  
MA Bourdon ◽  
GL Gilmore ◽  
RK Shadduck ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Line ◽  
Stromal Cell ◽  
Gel Filtration ◽  
Colony Stimulating Factor ◽  
Stromal Microenvironment ◽  
Stromal Cell Line ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

Knowledge of the stromal microenvironment is crucial for understanding the hematopoietic system. We took advantage of an assay that permits analysis of primary stroma-initiating cells (SICs) on the clonal level, and further characterized SICs and the factors that regulate SICs. Stroma formation in this assay is dependent on a high-molecular-weight factor secreted by the stromal cell line AC3.U. Here we show that this factor is identical to macrophage colony-stimulating factor (M-CSF), and that purified M-CSF is sufficient for induction of stroma formation. M-CSF, isolated from the line AC3.U, as well as from L929 cells and COS cells transfected with an expression vector encoding M-CSF, migrated in two peaks as 160- and 650-kD species after gel filtration. These molecular-weight species encompassed all stroma-inducing activity, and both stimulated macrophage colony formation. Affinity chromatography and blocking studies with antibodies specific for M-CSF and c-fms confirmed M-CSF as the sole factor in the supernatant of the stromal cell line AC3.U that promotes stroma formation. Culture of marrow, for as little as 1 week, depleted M-CSF-dependent SIC while increasing the incidence of replatable, factor-independent SIC. This suggests that culture changes the properties of SICs, perhaps by inducing differentiation into mature stromal cells. Thus, our results show a novel function of M-CSF as an important modulator of stroma formation.

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