Column chromatography fractionation of complex waste water sample extracts for measurement of ppt levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin

1981 ◽  
Vol 4 (4) ◽  
pp. 178-179 ◽  
Author(s):  
H. G. Nowicki ◽  
C. A. Kieda ◽  
V. Current ◽  
T. H. Schaefers
Keyword(s):  
Waste Water ◽  
Water Sample ◽  
Column Chromatography ◽  
Waste Water Sample ◽  
Tetrachlorodibenzo P Dioxin

scholarly journals Diversity of 4-Chloro-2-nitrophenol-Degrading Bacteria in a Waste Water Sample

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Pankaj Kumar Arora ◽  
Alok Srivastava ◽  
Vijay Pal Singh
Keyword(s):  
Waste Water ◽  
Water Sample ◽  
Mass Spectrometry Analysis ◽  
Contaminated Site ◽  
Gas Chromatography Mass Spectrometry ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Spectrometry Analysis ◽  
Waste Water Sample ◽  
Degrading Bacteria

Eighteen bacterial strains, isolated from a waste water sample collected from a chemically contaminated site, Patancheru (17°32′N 78°16′E/17.53°N 78.27°E), India, were able to decolorize 4-chloro-2-nitrophenol (4C2NP) in the presence of an additional carbon source. These eighteen 4C2NP-decolorizing strains have been identified as members of four different genera, includingBacillus,Paenibacillus,Pseudomonas, andLeuconostocbased on the 16S rRNA gene sequencing and phylogenetic analysis. Most of the bacteria (10) belonged to the genusBacillusand contributed 56% of the total 4C2NP-degrading bacteria, whereas the members of generaPaenibacillusandPseudomonasrepresented 22% and 17%, respectively, of total 4C2NP-degrading isolates. There was only one species ofLeuconostoccapable of degrading 4C2NP. This is the first report of the diversity of 4C2NP-decolorizing bacteria in a waste water sample. Furthermore, one bacterium,Bacillus aryabhattaistrain PC-7, was able to decolorize 4C2NP up to a concentration of 2.0 mM. Gas chromatography-mass spectrometry analysis identified 5-chloro-2-methylbenzoxazole as the final product of 4C2NP decolorization in strain PC-7.

scholarly journals Efektivitas pengolahan limbah cair hotel dan implementasi keputusan Menteri Lingkungan Hidup nomor 52/menlh/10/1995 tentang baku mutu limbah cair bagi kegiatan hotel di Kota Pekanbaru

Jurnal Zona ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Syafri Boy ◽  
Aras Mulyadi ◽  
Sukendi Sukendi
Keyword(s):  
Waste Water ◽  
Water Quality ◽  
Water Sample ◽  
Environmental Quality ◽  
Quality Standards ◽  
Quality Standard ◽  
Water Quality Standard ◽  
Environmental Quality Standards ◽  
Waste Water Sample ◽  
The City

This research aims to determine the effectiveness of the treatment of waste water hotel and see Implemantation Decree of the Minister of Environment No.52/MENLH/10/1995 concerning the Waste water Quality Hotel by five-star hotel in the city of Pekanbaru. The method used in this research is the survey and interview. Based on the analysis we found that the rate of decrease in the content of pollutants in the waste water hotel in reducing the content of pollutants in the waste water for parameters BOD, COD, TSS and pH respectively 72%, 75%, 25% and 16%. IPL Cability levels in the lower levels of waste water sample parameters for the parameters BOD, COD, TSS and pH respectively 60.0%, 30.0%, 50.0% and 87.5%. IPLC analysis results in Pekanbaru compared with the Waste water Quality Standard Hotel, pollutant levels are still above the environmental quality standards.

scholarly journals Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilicα-Amylase from an Obligate HalophilicAspergillus gracilis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Imran Ali ◽  
Ali Akbar ◽  
Benjawan Yanwisetpakdee ◽  
Sehanat Prasongsuk ◽  
Pongtharin Lotrakul ◽  
...  
Keyword(s):  
Waste Water ◽  
Water Activity ◽  
Column Chromatography ◽  
Specific Activity ◽  
Single Band ◽  
Water Remediation ◽  
Saline Waste ◽  
Sds Page ◽  
Nacl Concentration ◽  
Burk Plot

An obligate halophilicAspergillus graciliswhich was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellularα-amylase in the previous studies. In this study theα-amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa. The specific activity of the enzyme was found to be 131.02 U/mg. The Lineweaver-Burk plot showed theVmaxandKmvalues of 8.36 U/mg and 6.33 mg/mL, respectively. The enzyme was found to have the best activity at 5 pH, 60°C, and 30% of NaCl concentration, showing its polyextremophilic nature. The use of various additives did not show much variation in the activity of enzyme, showing its resilience against inhibitors. The enzyme, when tested for its use for synthetic waste water remediation by comparing its activity with commercial amylase in different salt concentrations showed that theα-amylase fromA. graciliswas having better performance at increasing salt concentrations than the commercial one. This shows its potential to be applied in saline waste water and other low water activity effluents for bioremediation.

Treatment of Waste Seawater from Shrimp Farm Using a Photocatalytic Membrane Reactor

2013 ◽  
Vol 409-410 ◽  
pp. 199-203 ◽  
Author(s):  
Zai Feng Shi ◽  
Su Min Zhang ◽  
Su Guo
Keyword(s):  
Waste Water ◽  
Particle Size ◽  
Membrane Reactor ◽  
Membrane Filtration ◽  
Ceramic Membrane ◽  
Permeation Flux ◽  
Waste Water Sample ◽  
Operation Conditions ◽  
Shrimp Farms ◽  
Photocatalytic Membrane Reactor

To treat the waste seawater from shrimp farms, TiO2 powder photocatalysis and ceramic membrane filtration technologies were integrated to form a photocatalytic membrane reactor (PMR). PMR performance was investigated and real waste water sample was treated under the optimum operation conditions. Results indicated that TiO2 powder with mean particle size of 0.27 μm could be separated successfully with α-Al2O3 membrane with pore size of 0.05 μm, the permeation flux was 432 Lhr-1m-2 under pressure of 0.05 MPa. Wastewater was treated with three different catalysts involving 2 g of TiO2 powder, 2 g of TiO2 powder combined with 5 ml of H2O2 (30%), and 2 g of TiO2 powder combined with 5 ml of Fenton agent for per liter of wastewater, the corresponding CODCr removal rates were 15.67 %, 25.19 % and 40.67 % separately.

scholarly journals POTENTIAL OF WATER HYACINTH (EICHHORNIA CRASSIPES L.) FOR PHYTOREMEDIATION OF HEAVY METALS FROM WASTE WATER

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
MI Nazir ◽  
I Idrees ◽  
P Idrees ◽  
S Ahmad ◽  
Q Ali ◽  
...  
Keyword(s):  
Waste Water ◽  
Heavy Metals ◽  
Water Sample ◽  
Water Hyacinth ◽  
Eichhornia Crassipes ◽  
Metal Uptake ◽  
Room Temperature ◽  
Dry Weight ◽  
Sewage Water ◽  
Plant Sample

The present study was carried out to determine the potential for phytoremediation of water hyacinth (Eichhornia crassipes) plant for Cadmium (Cd), Arsenic (Ar), and Mercury (Hg) absorption. The samples were collected from Dhamthal, Zafarwal and Narowal. The plant samples were cut into their parts and dried at room temperature for 25-30 days until they were fully dried. The change in fresh weight and dry weight was examined. The data of collected samples was recorded and statistically analyzed, which revealed the significance of results for different localities. The lower coefficient of variation was recorded for all studied traits which revealed that there was consistency among the results for different localities. For our study the plant's percentage removal of metals was determined using atomic absorption spectroscopy in plant sample as well as water sample. Metal uptake happened at variable degrees. The water hyacinth uptake the largest metal uptake per dry weight of water hyacinth was 166.25ppm for cadmium and the smallest 0.032ppm was for mercury. In water sample highest amount of metal was 177.25ppm for cadmium and lowest 0.012ppm was for arsenic. It was found from our study that the water hyacinth (Eichhornia crasssipes) uptake cadmium (cd) metal from sewage water in highest amount as compare to arsenic and mercury. It was suggested that the use of water hyacinth plant may be helpful to remove heavy metals from waste water to minimize the heavy metal pollution of water.

scholarly journals Isolation and optimization of the fermentation condition of cellulolytic microbial isolates from cassava waste water

2021 ◽  
Vol 14 (1) ◽  
pp. 011-017
Author(s):  
Yaya A. Gimba ◽  
Abubakar Idris ◽  
Abdullahi Hassan ◽  
Opeyemi N. Hassan
Keyword(s):  
Waste Water ◽  
Bacillus Subtilis ◽  
Aspergillus Niger ◽  
Cellulase Activity ◽  
Maximum Activity ◽  
Alternative Source ◽  
Waste Water Sample ◽  
Microbiological Methods ◽  
Processing Water ◽  
Cassava Waste

The study was conducted to isolate and identify cellulose producing microorganisms from cassava waste water. Cassava waste water sample was obtained from a cassava processing factory at Lokogoma town in Wushishi Local government area of Niger State. The microorganisms were isolated, identified and counted by standard microbiological methods. The mean bacteria count ranges from 6.8 x 103 cfu/mL-1 to 2.1 x 103 cfu/mL-1 while the fungi count ranges from 3.2 x 103 cfu/mL-1 to 1.2 x 103 cfu/mL-1. A total of eight (8) bacterial; Staphylococcus aureus, Bacillus anthrax, Bacillus subtilis, Escherichia coli, Klebsilla sp, Bacillus megaterus, Staph. Epidermidis and Pseudomonas aeruginosa, and six (6) fungi; Saccharomyce serivicea, Aspergillus niger, Penecillium sp., Muccor sp., Aspergilus flavus and Aspergilus fumigetus isolates were identified in the waste water. Among these organism, the best cellulase activity was recorded for Bacillus subtilis (10.39 x 10-4 mg/ml/sec) and Aspergillus niger (11.21 x 10-4 mg/ml/sec). However, maximum activity was obtained at pH ranges from 3 ~ 9, temperature ranges from 30 oC ~ 80oC and substrate concentrations ranges from 1.5% ~3.0%. In conclusion, cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.

Identification of Asbestos Fibres in a Water Sample by X-Ray Microanalysis

1975 ◽  
Vol 33 ◽  
pp. 274-275
Author(s):  
K. A. Brookes ◽  
D. Finbow ◽  
Madeleine Samuel
Keyword(s):  
Water Sample ◽  
Background Radiation ◽  
X Ray ◽  
Left Hand ◽  
Transmission Imaging ◽  
Different Types ◽  
Normal Specimen ◽  
Liquid Nitrogen Cooling ◽  
Normal Transmission ◽  
Additional Port

Investigation of the particulate matter contained in the water sample, revealed the presence of a number of different types and certain of these were selected for analysis.An A.E.I. Corinth electron microscope was modified to accept a Kevex Si (Li) detector. To allow for existing instruments to be readily modified, this was kept to a minimum. An additional port is machined in the specimen region to accept the detector, with the liquid nitrogen cooling dewar conveniently housed in the left hand cupboard adjacent to the microscope column. Since background radiation leads to loss in the sensitivity of the instrument, great care has been taken to reduce this effect by screening and manufacturing components that are near the specimen from material of low atomic number. To change from normal transmission imaging to X-ray analysis, the special 4-position specimen rod is inserted through the normal specimen airlock.

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