Synthesis, Cell Viability, and Flow Cytometric Fluorescence Pulse Width Analysis of Dendrimers with Indazoles Surface Unit

2017 ◽  
Vol 54 (6) ◽  
pp. 3042-3050
Author(s):  
Mani Jayanthi ◽  
Perumal Rajakumar
Keyword(s):  
Cell Viability ◽  
Pulse Width ◽  
Flow Cytometric ◽  
Surface Unit ◽  
Fluorescence Pulse

scholarly journals Flow cytometric fluorescence pulse width analysis of etoposide-induced nuclear enlargement in HCT116 cells

2010 ◽  
Vol 32 (8) ◽  
pp. 1045-1052 ◽  
Author(s):  
Kyungsu Kang ◽  
Saet Byoul Lee ◽  
Ji-Hye Yoo ◽  
Chu Won Nho
Keyword(s):  
Pulse Width ◽  
Flow Cytometric ◽  
Fluorescence Pulse ◽  
Hct116 Cells ◽  
Nuclear Enlargement

The Clinical Significance of miR-135b-5p and Its Role in the Proliferation and Apoptosis of Hippocampus Neurons in Children with Temporal Lobe Epilepsy

2020 ◽  
Vol 42 (5-6) ◽  
pp. 187-194
Author(s):  
Ruixiang Li ◽  
Jiahua Hu ◽  
Sue Cao
Keyword(s):  
Temporal Lobe Epilepsy ◽  
Cell Viability ◽  
Temporal Lobe ◽  
Luciferase Reporter ◽  
Diagnostic Biomarker ◽  
Flow Cytometric ◽  
Proliferation And Apoptosis ◽  
Apoptosis Assay ◽  
Polymerase Chain ◽  
Adverse Influence

Temporal lobe epilepsy (TLE) is the most familiar localized epilepsy in children. MicroRNAs (miRNAs) are essential for the inhibition or promotion of numerous diseases. This study aimed to detect the expression of miR-135b-5p and primarily uncover its underlying function and mechanism in children with TLE. Quantitative real-time polymerase chain reaction was used to evaluate the expression of miR-135b-5p in children with TLE and in a rat model of epilepsy. MTT assay and flow cytometric apoptosis assay were conducted to evaluate the effects of miR-135b-5p on cell viability and apoptosis. Additionally, the dual luciferase reporter assay was performed to confirm the direct target of miR-135b-5p. Our data showed that the expression of miR-135b-5p was significantly decreased in children with TLE and in the epileptic rat neuron model. The dysregulation of miR-135b-5p could serve as a promising diagnostic biomarker for children with TLE. The overexpression of miR-135b-5p moderated the adverse influence on cell viability and apoptosis induced by magnesium-free medium. SIRT1 was identified as a target gene of miR-135b-5p. These results proved that miR-135b-5p might serve as a potential diagnostic biomarker in children with TLE. Overexpression of miR-135b-5p alleviates the postepileptic influence on cell viability and apoptosis by targeting SIRT1.

Theoretical and Experimental Study on Parametric Fluorescence Pulse Width

2016 ◽  
Vol 36 (5) ◽  
pp. 0519001
Author(s):  
王波鹏 Wang Bopeng ◽  
粟敬钦 Su Jingqin ◽  
曾小明 Zeng Xiaoming ◽  
王晓东 Wang Xiaodong ◽  
王逍 Wang Xiao ◽  
...  
Keyword(s):  
Experimental Study ◽  
Pulse Width ◽  
Fluorescence Pulse

Evaluation of fluorescein diacetate for flow cytometric determination of cell viability in orthopaedic research

1988 ◽  
Vol 6 (4) ◽  
pp. 467-474 ◽  
Author(s):  
R. S. Bell ◽  
L. A. Bourret ◽  
D. F. Bell ◽  
M. C. Gebhardt ◽  
A. Rosenberg ◽  
...  
Keyword(s):  
Cell Viability ◽  
Fluorescein Diacetate ◽  
Flow Cytometric ◽  
Orthopaedic Research

scholarly journals Correlation between Electrical Field Strength and Pulse Width Analysis on Cell Viability

2018 ◽  
Vol 1019 ◽  
pp. 012012 ◽  
Author(s):  
Suhassni Ganeson ◽  
Muhammad Mahadi Abdul Jamil ◽  
Hassan Buhari Mamman ◽  
Nur Adilah Abd Rahman
Keyword(s):  
Field Strength ◽  
Cell Viability ◽  
Pulse Width ◽  
Electrical Field ◽  
Electrical Field Strength

A rapid analytical technique for flow cytometric analysis of cell viability using calcofluor white M2R

Cytometry ◽  
1987 ◽  
Vol 8 (4) ◽  
pp. 421-426 ◽  
Author(s):  
Deborah L. Berglund ◽  
Rolf E. Taffs ◽  
Nancy P. Robertson
Keyword(s):  
Cell Viability ◽  
Flow Cytometric Analysis ◽  
Calcofluor White ◽  
Analytical Technique ◽  
Flow Cytometric

scholarly journals A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells

2019 ◽  
Vol 37 (1) ◽  
Author(s):  
Harrison J. Wensley ◽  
David A. Johnston ◽  
Wendy S. Smith ◽  
Suzanne E. Holmes ◽  
Sopsamorn U. Flavell ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Pulse Width ◽  
Endosomal Escape ◽  
Target Cells ◽  
Triterpenoid Saponin ◽  
Simple Method ◽  
Flow Cytometric Method ◽  
Protein Toxin ◽  
Flow Cytometric ◽  
Width Measurements

Abstract Purpose The aim of this work was to develop a quantitative, flow cytometric method for tracking the endolysosomal escape of a fluorescently labelled saporin toxin. Methods Flow cytometric measurements of fluorescent pulse width and height were used to track the endocytic uptake into Daudi cells of a fluorescently labelled saporin toxin and the saporin based immunotoxin, OKT10-SAP. Subsequently, measurement of changes in pulse width were used to investigate the effect of a triterpenoid saponin on the endolysosomal escape of internalised toxin into the cytosol. Live cell confocal microscopy was used to validate the flow cytometry data. Results Increased endolysosomal escape of saporin and OKT10-SAP was observed by confocal microscopy in cells treated with saponin. Fluorescent pulse width measurements were also able to detect and quantify escape more sensitively than confocal microscopy. Saponin induced endolysosomal escape could be abrogated by treatment with chloroquine, an inhibitor of endolysosomal acidification. Chloroquine abrogation of escape was also mirrored by a concomitant abrogation of cytotoxicity. Conclusions Poor endolysosomal escape is often a rate limiting step for the cytosolic delivery of protein toxins and other macromolecules. Pulse width analysis offers a simple method to semi-quantify the endolysosomal escape of this and similar molecules into the cytosol.

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