Flow cytometric lifetime-based cell viability assay using propidium iodide

1999 ◽  
Author(s):  
John A. Steinkamp ◽  
Bruce E. Lehnert ◽  
Nancy M. Lehnert
Keyword(s):  
Cell Viability ◽  
Propidium Iodide ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Flow Cytometric

Do We have a Satisfactory Cell Viability Assay? Review of the Currently Commercially-Available Assays

2020 ◽  
Vol 17 (1) ◽  
pp. 2-22 ◽  
Author(s):  
Abdel-Baset Halim
Keyword(s):  
Cell Viability ◽  
Data Interpretation ◽  
Positive Control ◽  
Live Cells ◽  
Proof Of Concept ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Current Cell ◽  
Dying Cells ◽  
Differential Permeability

:Cell-based assays are an important part of the drug discovery process and clinical research. One of the main hurdles is to design sufficiently robust assays with adequate signal to noise parameters while maintaining the inherent physiology of the cells and not interfering with the pharmacology of target being investigated.:A plethora of assays that assess cell viability (or cell heath in general) are commercially available and can be classified under different categories according to their concepts and principle of reactions. The assays are valuable tools, however, suffer from a large number of limitations. Some of these limitations can be procedural or operational, but others can be critical as those related to a poor concept or the lack of proof of concept of an assay, e.g. those relying on differential permeability of dyes in-and-out of viable versus compromised cell membranes. While the assays can differentiate between dead and live cells, most, if not all, of them can just assess the relative performance of cells rather than providing a clear distinction between healthy and dying cells. The possible impact of relatively high molecular weight dyes, used in most of the assay, on cell viability has not been addressed. More innovative assays are needed, and until better alternatives are developed, setup of current cell-based studies and data interpretation should be made with the limitations in mind. Negative and positive control should be considered whenever feasible. Also, researchers should use more than one orthogonal method for better assessment of cell health.

scholarly journals Promising biocompatible hydrogels of crosslinked polyelectrolytes for biomedical applications

2017 ◽  
Vol 3 (2) ◽  
pp. 695-698
Author(s):  
Andreas Brietzke ◽  
Christian von der Ehe ◽  
Sabine Illner ◽  
Claudia Matschegewski ◽  
Niels Grabow ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Ionic Liquids ◽  
Cell Viability ◽  
Biomedical Applications ◽  
High Potential ◽  
Cell Viability Assay ◽  
Mouse Fibroblasts ◽  
Viability Assay ◽  
Intelligent Implant ◽  
L929 Mouse

AbstractFor the development of intelligent implant systems hydrogels (HG) from crosslinked ionic liquids feature a high potential to be utilised as a drug depot. Biocompatibility of the HGs is one key prerequisite for biomedical applications. HGs were polymerised from a variety of different ionic monomers based on methacrylate, methacrylamide, styrene or vinyl imidazolium derivatives in aqueous solution. N,N'-methylenebisacrylamide was used as crosslinker. CellQuanti-Blue™ Cell Viability Assay Kit was implemented to proof viability of L929 mouse fibroblasts. The predominant part of the HG eluates generated only a marginal reduction of less than 15% cell viability at 100% eluate concentration. This underlines the excellent suitability of these HGs for biomedical applications and revealed some promising candidates for the development of drug depots for implants.

scholarly journals Sensitive cell viability assay for use in drug screens and for studying the mechanism of action of drugs inDictyostelium discoideum

BioTechniques ◽  
2006 ◽  
Vol 41 (5) ◽  
pp. 591-595 ◽  
Author(s):  
Junxia Min ◽  
Priya Sridevi ◽  
Stephen Alexander ◽  
Hannah Alexander
Keyword(s):  
Cell Viability ◽  
Mechanism Of Action ◽  
Sensitive Cell ◽  
Cell Viability Assay ◽  
Viability Assay

scholarly journals Synthesis of Novel FTY720 Analogs with Anticancer Activity through PP2A Activation

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2750 ◽  
Author(s):  
Jitendra Shrestha ◽  
Sung Ki ◽  
Sang Shin ◽  
Seon Kim ◽  
Joo-Youn Lee ◽  
...  
Keyword(s):  
Cell Viability ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Sphingosine Kinase ◽  
Basic Structure ◽  
Docking Study ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Anticancer Effects ◽  
Pp2a Activation

FTY720 inhibits various cancers through PP2A activation. The structure of FTY720 is also used as a basic structure for the design of sphingosine kinase (SK) inhibitors. We have synthesized derivatives using an amide chain in FTY720 with a phenyl backbone, and then compounds were screened by an MTT cell viability assay. The PP2A activity of compound 7 was examined. The phosphorylation levels of AKT and ERK, downstream targets of PP2A, in the presence of compound 7, were determined. Compound 7 may exhibit anticancer effects through PP2A activation rather than the mechanism by inhibition of SK1 in cancer cells. In the docking study of compound 7 and PP2A, the amide chain of compound 7 showed an interaction with Asn61 that was different from FTY720, which is expected to affect the activity of the compound.

Evaluation of Paclitaxel (Taxol), Cisplatin, and the Combination Paclitaxel-Cisplatin in Ovarian Cancer in Vitro with the ATP Cell Viability Assay

1994 ◽  
Vol 53 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Michael Untch ◽  
Bernd-Uwe Sevin ◽  
James P. Perras ◽  
Roberto Angioli ◽  
Andrea Untch ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Viability ◽  
Cell Viability Assay ◽  
Viability Assay

scholarly journals Noninvasive and safe cell viability assay forEuglena gracilisusing natural food pigment

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6636 ◽  
Author(s):  
Kyohei Yamashita ◽  
Koji Yamada ◽  
Kengo Suzuki ◽  
Eiji Tokunaga
Keyword(s):  
Cell Viability ◽  
Euglena Gracilis ◽  
Food Culture ◽  
Live Cells ◽  
Natural Food ◽  
Synthetic Dyes ◽  
Cell Viability Assay ◽  
Natural Pigments ◽  
Viability Assay ◽  
Good Ability

Noninvasive and safe cell viability assay is required in many fields such as regenerative medicine, genetic engineering, single-cell analysis, and microbial food culture. In this case, a safe and inexpensive method which is a small load on cells and the environment is preferable without requiring expensive and space-consuming equipment and a technician to operate. We examined eight typical natural food pigments to findMonascuspigment (MP) or anthocyanin pigment (AP) works as a good viability indicator of dye exclusion test (DET) forEuglena graciliswhich is an edible photosynthetic green microalga. This is the first report using natural food pigments as cell viability assay.Euglena gracilisstained by MP or AP can be visually judged with a bright field microscope. This was spectrally confirmed by scan-free, non-invasive absorbance spectral imagingA(x, y,λ) microscopy of single live cells and principal component analysis (PCA). To confirm the ability of staining dead cells and examine the load on the cells, these two natural pigments were compared with trypan blue (TB) and methylene blue (MP), which are synthetic dyes conventionally used for DET. As a result, MP and AP had as good ability of staining dead cells treated with microwave as TB and MB and showed faster and more uniform staining for dead cells in benzalkonium chloride than them. The growth curve and the ratio of dead cells in the culture showed that the synthetic dyes inhibit the growth ofE. gracilis, but the natural pigments do not. As the cell density increased, however, AP increased the ratio of stained cells, which was prevented by the addition of glucose. MP can stain dead cells in a shorter time than AP, while AP is more stable in color against long-term irradiation of intense light than MP. Due to the low toxicity of these pigments, viability of cells in culture can be monitored with them over a long period.

scholarly journals Label-free cell viability assay using phase imaging with computational specificity

2020 ◽  
Author(s):  
Chenfei Hu ◽  
Shenghua He ◽  
Young Jae Lee ◽  
Yuchen He ◽  
Edward M. Kong ◽  
...  
Keyword(s):  
Cell Viability ◽  
Hela Cells ◽  
Free Cell ◽  
Phase Imaging ◽  
Label Free ◽  
Cell Dynamics ◽  
Cell Viability Assay ◽  
Chemical Reagents ◽  
Viability Assay ◽  
Cell Staining

AbstractExisting approaches to evaluate cell viability involve cell staining with chemical reagents. However, this step of exogenous staining makes these methods undesirable for rapid, nondestructive and long term investigation. Here, we present instantaneous viability assessment of unlabeled cells using phase imaging with computation specificity (PICS). This new concept utilizes deep learning techniques to compute viability markers associated with the specimen measured by quantitative phase imaging. Demonstrated on HeLa cells culture, the proposed method reports approximately 95% accuracy in identifying injured and dead cells. Further comparison of cell morphology with labeled HeLa cells suggests that potential adverse effect on cell dynamics introduced by the viability reagents can be avoided using the label-free investigation method, which would be valuable for a broad range of biomedical applications.

scholarly journals Efek Sitotoksik Ekstrak Daun Ketapang (Terminalia catappa L.) Pada Sel Kanker Payudara T47D

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Barinta Widaryanti ◽  
Nur Khikmah ◽  
Nunung Sulistyani
Keyword(s):  
Cancer Therapy ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Breast Cancer Cells ◽  
Methanol Extract ◽  
Cell Viability Assay ◽  
Key Words Cancer ◽  
T47d Cells ◽  
Viability Assay ◽  
The World

Cancer is the second cause of death in the world after heart disease. Cancer therapy using natural product was considered has no side effect. Secondary metabolites from ketapang (Terminalia cattapa L) leaves has a prospect for cancer therapy. The objective of this research was to determine the cytotoxical effect of ketapang leaves on T47D breast cancer cells. Ketapang leaves were macerated using chloroform, ethyl acetate and methanol. Cytotoxicity effect were determined using MTT Cell Viability Assay. The result showed that chloroform, ethyl acetate and methanol extract were not able to reduce cell viability of T47D cells. Key words: cancer, ketapang, T. cattapa, cytotoxic, T47D cells.

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