LIM kinase-2 targeting as a possible anti-metastasis therapy

2004 ◽  
Vol 6 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Eigo Suyama ◽  
Renu Wadhwa ◽  
Hiroaki Kawasaki ◽  
Tomoko Yaguchi ◽  
Sunil C. Kaul ◽  
...  
Keyword(s):  
Lim Kinase ◽  
Metastasis Therapy

LIM kinase-1 in the cerebral ganglion of Drosophila with genetic disturbances of kynurenine balance

2008 ◽  
Vol 418 (1) ◽  
pp. 1-3 ◽  
Author(s):  
N. G. Lopatina ◽  
T. G. Zachepilo ◽  
E. V. Savvateeva-Popova
Keyword(s):  
Cerebral Ganglion ◽  
Lim Kinase ◽  
Lim Kinase 1

scholarly journals Visualization and Molecular Analysis of Actin Assembly in Living Cells

1998 ◽  
Vol 143 (7) ◽  
pp. 1919-1930 ◽  
Author(s):  
Dorothy A. Schafer ◽  
Matthew D. Welch ◽  
Laura M. Machesky ◽  
Paul C. Bridgman ◽  
Shelley M. Meyer ◽  
...  
Keyword(s):  
Actin Filament ◽  
Eukaryotic Cell ◽  
Cell Periphery ◽  
Actin Assembly ◽  
Cortical Actin ◽  
Lim Kinase ◽  
Permeabilized Cells ◽  
Capping Protein ◽  
Filament Assembly

Actin filament assembly is critical for eukaryotic cell motility. Arp2/3 complex and capping protein (CP) regulate actin assembly in vitro. To understand how these proteins regulate the dynamics of actin filament assembly in a motile cell, we visualized their distribution in living fibroblasts using green flourescent protein (GFP) tagging. Both proteins were concentrated in motile regions at the cell periphery and at dynamic spots within the lamella. Actin assembly was required for the motility and dynamics of spots and for motility at the cell periphery. In permeabilized cells, rhodamine-actin assembled at the cell periphery and at spots, indicating that actin filament barbed ends were present at these locations. Inhibition of the Rho family GTPase rac1, and to a lesser extent cdc42 and RhoA, blocked motility at the cell periphery and the formation of spots. Increased expression of phosphatidylinositol 5-kinase promoted the movement of spots. Increased expression of LIM–kinase-1, which likely inactivates cofilin, decreased the frequency of moving spots and led to the formation of aggregates of GFP–CP. We conclude that spots, which appear as small projections on the surface by whole mount electron microscopy, represent sites of actin assembly where local and transient changes in the cortical actin cytoskeleton take place.

scholarly journals LIM kinase 1 serves an important role in the multidrug resistance of osteosarcoma cells

2017 ◽  
Author(s):  
Jian‑Zeng Yang ◽  
Li‑Hong Huang ◽  
Rui Chen ◽  
Ling‑Jie Meng ◽  
Yi‑Yao Gao ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Osteosarcoma Cells ◽  
Lim Kinase ◽  
Lim Kinase 1

scholarly journals A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208979 ◽  
Author(s):  
Renaud Prudent ◽  
Nathalie Demoncheaux ◽  
Hélène Diemer ◽  
Véronique Collin-Faure ◽  
Reuben Kapur ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Kinase Inhibitor ◽  
Myeloid Cells ◽  
Lim Kinase ◽  
Quantitative Proteomic Analysis ◽  
Cofilin Phosphorylation
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