Long-term control of food intake and body weight by hydrodynamics-based delivery of plasmid DNA encoding leptin or CNTF

2003 ◽  
Vol 5 (11) ◽  
pp. 977-983 ◽  
Author(s):  
Jingjing Jiang ◽  
Eiji Yamato ◽  
Jun-ichi Miyazaki
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Plasmid Dna ◽  
Dna Encoding

scholarly journals Release of Plasmid DNA-Encoding IL-10 from PLGA Microparticles Facilitates Long-Term Reversal of Neuropathic Pain Following a Single Intrathecal Administration

2010 ◽  
Vol 27 (5) ◽  
pp. 841-854 ◽  
Author(s):  
Ryan Gene Soderquist ◽  
Evan M. Sloane ◽  
Lisa C. Loram ◽  
Jacqueline A. Harrison ◽  
Ellen C. Dengler ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Plasmid Dna ◽  
Intrathecal Administration ◽  
Dna Encoding ◽  
Plga Microparticles

Long-term effects of TRH administration on food intake and body weight in the rat

1986 ◽  
Vol 24 (6) ◽  
pp. 1817-1819 ◽  
Author(s):  
R. Iglesias ◽  
M. Llobera ◽  
E. Montoya
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Long Term Effects

Glucocorticoids and insulin: reciprocal signals for energy balance

1995 ◽  
Vol 268 (1) ◽  
pp. R142-R149 ◽  
Author(s):  
A. M. Strack ◽  
R. J. Sebastian ◽  
M. W. Schwartz ◽  
M. F. Dallman
Keyword(s):  
Body Weight ◽  
Energy Balance ◽  
Food Intake ◽  
Diabetic Rats ◽  
Energy Gain ◽  
Energy Stores ◽  
The Central Nervous System ◽  
Streptozotocin Induced Diabetes ◽  
Term Energy

Signals that regulate long-term energy balance have been difficult to identify. Increasingly strong evidence indicates that insulin, acting on the central nervous system in part through its effect on neuropeptide Y (NPY), inhibits food intake. We hypothesized that corticosteroids and insulin might serve as interacting, reciprocal signals for energy balance, acting on energy acquisition, in part through their effects on hypothalamic NPY, as well as on energy stores. Because glucocorticoids also stimulate insulin secretion, their role is normally obscured. Glucocorticoids and insulin were clamped in adrenalectomized rats with steroid replacement and streptozotocin-induced diabetes. Glucocorticoids stimulated and insulin inhibited NPY mRNA and food intake. Glucocorticoids inhibited and insulin increased energy gain as determined by the change in body weight. When adrenalectomized diabetic rats were treated, corticosterone stimulated and insulin inhibited food intake, and, respectively, inhibited and increased overall energy gain. More than 50% of the variance was explained by regression analysis of the two hormones on food intake and body weight. Thus glucocorticoids and insulin are major, antagonistic, long-term regulators of energy balance. The effects of corticosterone and insulin on food intake may be mediated, in part, through regulation of hypothalamic NPY synthesis and secretion.

Effects of lyoprotectants on long-term stability and transfection efficacy of lyophilized poly(lactide-co-glycolide)-graft-polyethylenimine/plasmid DNA polyplexes

Nanomedicine ◽  
2021 ◽  
Author(s):  
Joshua Woo ◽  
Jeoung Soo Lee
Keyword(s):  
Plasmid Dna ◽  
Fluorescent Protein ◽  
Transfection Efficiency ◽  
Dna Encoding ◽  
Rat Glioma ◽  
Term Stability ◽  
Long Term Stability ◽  
Glioma C6 ◽  
Transfection Efficacy

Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polyplexes prepared from poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) and plasmid DNA encoding green fluorescent protein (pGFP). Materials & methods: Lyo. PgP/pGFP polyplexes prepared with/without lyoprotectants were stored at -20°C over 6 months. Polyplex stability was analyzed by gel electrophoresis and heparin competition assay. Transfection efficiency and cytotoxicity were evaluated in rat glioma (C6) cells in medium containing 10% serum. Results: Lyo. PgP/pGFP polyplexes prepared with 5% sucrose as a lyoprotectant remained stable up to 6 months and retained transfection efficiency up to 4 months. Conclusion: Lyo. PgP-based polyplexes retain bioactivity during extended storage, potentially enabling transport to remote regions and less stable settings, increasing access to life-changing gene therapy.

scholarly journals Variations in body weight, food intake and body composition after long-term high-fat diet feeding in C57BL/6J mice

Obesity ◽  
2014 ◽  
Vol 22 (10) ◽  
pp. 2147-2155 ◽  
Author(s):  
Yongbin Yang ◽  
Daniel L. Smith ◽  
Karen D. Keating ◽  
David B. Allison ◽  
Tim R. Nagy
Keyword(s):  
Body Composition ◽  
Body Weight ◽  
Food Intake ◽  
High Fat Diet ◽  
High Fat

Hypothalamic control of photoperiod-induced cycles in food intake, body weight, and metabolic hormones in rams

2001 ◽  
Vol 281 (1) ◽  
pp. R76-R90 ◽  
Author(s):  
Gerald A. Lincoln ◽  
Stewart M. Rhind ◽  
Sueli Pompolo ◽  
Iain J. Clarke
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Sheep Model ◽  
Blood Concentrations ◽  
Metabolic Hormones ◽  
Melanocyte Stimulating Hormone ◽  
Induced Changes ◽  
And Control ◽  
Short Days

This study used a hypothalamo-pituitary disconnected (HPD) sheep model to investigate the central regulation of long-term cycles in voluntary food intake (VFI) and body weight (BW). VFI, BW, and circulating concentrations of metabolic hormones [α-melanocyte-stimulating hormone (α-MSH), insulin-like growth factor-1 (IGF-1), insulin, and leptin] were measured in HPD and control Soay rams exposed to alternating 16 weekly periods of long and short days for 80 wk. In the controls, the physiology was cyclical with a 32-wk periodicity corresponding to the lighting regimen. VFI and BW increased under long days to a maximum early into short days, and there were associated increases in blood concentrations of α-MSH, insulin, and leptin. In the HPD rams, there were no significant photoperiod-induced changes in any of the parameters. VFI increased after surgery for 8 wk and then gradually declined, although BW increased progressively and the HPD rams became obese. Concentrations of α-MSH, insulin, and leptin in peripheral blood were permanently increased (>200%), and levels of IGF-1 decreased (<55%). The HPD lesion effectively destroyed the entire median eminence [no nerve terminals immunostained for tyrosine hydroxylase (TH) and gonadotropin-releasing hormone] and the adjacent arcuate nucleus (no perikarya immunostained for proopiomelanocortin or TH, and no cells expressed neuropeptide Y mRNA). The results support the conclusion that arcuate hypothalamic systems generate long-term rhythms in VFI, BW, and energy balance.

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