Cationic micelle: A promising nanocarrier for gene delivery with high transfection efficiency

2019 ◽  
Vol 21 (7) ◽  
Author(s):  
Haili Wang ◽  
Shenggang Ding ◽  
Ze Zhang ◽  
Longhai Wang ◽  
Yezi You
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
High Transfection Efficiency

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

2019 ◽  
Vol 30 (10) ◽  
pp. 2567-2576
Author(s):  
Xinghong Duo ◽  
Lingchuang Bai ◽  
Jun Wang ◽  
Hao Ji ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Delivery System ◽  
High Transfection Efficiency

Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery

Biomaterials ◽  
2007 ◽  
Vol 28 (32) ◽  
pp. 4889-4900 ◽  
Author(s):  
May P. Xiong ◽  
M. Laird Forrest ◽  
Giangthy Ton ◽  
Anni Zhao ◽  
Neal M. Davies ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
High Transfection Efficiency ◽  
Low Toxicity

Highly Branched Poly(β-amino esters) for Non-Viral Gene Delivery: High Transfection Efficiency and Low Toxicity Achieved by Increasing Molecular Weight

2016 ◽  
Vol 17 (11) ◽  
pp. 3640-3647 ◽  
Author(s):  
Yongsheng Gao ◽  
Jian-Yuan Huang ◽  
Jonathan O’Keeffe Ahern ◽  
Lara Cutlar ◽  
Dezhong Zhou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Viral Gene ◽  
Amino Esters ◽  
High Transfection Efficiency ◽  
Low Toxicity ◽  
Viral Gene Delivery

Erythrocyte membrane based cationic polymer-mcDNA complexes as an efficient gene delivery system

2017 ◽  
Vol 5 (1) ◽  
pp. 120-127 ◽  
Author(s):  
Ping Huang ◽  
Jing Zhao ◽  
Chiju Wei ◽  
Xiaohu Hou ◽  
Pingzhang Chen ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Erythrocyte Membrane ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Cationic Polymer ◽  
Gene Delivery System ◽  
High Transfection Efficiency ◽  
System P ◽  
Efficient Gene

An erythrocyte membrane based gene delivery system presents high transfection efficiency and negligible cytotoxicity.

Guanidine-rich helical polypeptides bearing hydrophobic amino acid pendants for efficient gene delivery

2021 ◽  
Author(s):  
Zikun Yu ◽  
Zhimin Zhang ◽  
Jing Yan ◽  
Ziyin Zhao ◽  
Chenglong Ge ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Amino Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Hydrophobic Amino Acid ◽  
High Transfection Efficiency ◽  
Efficient Gene ◽  
Low Cytotoxicity

Guanidine-rich helical polypeptides bearing hydrophobic amino acid pendants displayed high transfection efficiency both in vitro and in vivo and low cytotoxicity toward applications in gene therapy.

Red-blood-cell-mimetic gene delivery systems for long circulation and high transfection efficiency in ECs

2018 ◽  
Vol 6 (37) ◽  
pp. 5975-5985 ◽  
Author(s):  
Xuefang Hao ◽  
Qian Li ◽  
Huaning Wang ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Blood Cell ◽  
Immune Evasion ◽  
Red Blood Cell ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
High Transfection Efficiency

Red-blood-cell-biomimetic gene delivery systems exhibit long circulation, strong immune evasion and high transfection efficiency.

Stepwise Development of Biomimetic Chimeric Peptides for Gene Delivery

2020 ◽  
Vol 27 (8) ◽  
pp. 698-710
Author(s):  
Roya Cheraghi ◽  
Mahboobeh Nazari ◽  
Mohsen Alipour ◽  
Saman Hosseinkhani
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Target Cells ◽  
Scale Production ◽  
Stepwise Development ◽  
Chimeric Peptides

Gene-based therapy largely relies on the vector type that allows a selective and efficient transfection into the target cells with maximum efficacy and minimal toxicity. Although, genes delivered utilizing modified viruses transfect efficiently and precisely, these vectors can cause severe immunological responses and are potentially carcinogenic. A promising method of overcoming this limitation is the use of non-viral vectors, including cationic lipids, polymers, dendrimers, and peptides, which offer potential routes for compacting DNA for targeted delivery. Although non-viral vectors exhibit reduced transfection efficiency compared to their viral counterpart, their superior biocompatibility, non-immunogenicity and potential for large-scale production make them increasingly attractive for modern therapy. There has been a great deal of interest in the development of biomimetic chimeric peptides. Biomimetic chimeric peptides contain different motifs for gene translocation into the nucleus of the desired cells. They have motifs for gene targeting into the desired cell, condense DNA into nanosize particles, translocate the gene into the nucleus and enhance the release of the particle into the cytoplasm. These carriers were developed in recent years. This review highlights the stepwise development of the biomimetic chimeric peptides currently being used in gene delivery.

scholarly journals Highly Osmotic Oxidized Sucrose-Crosslinked Polyethylenimine for Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 87
Author(s):  
Jaehong Park ◽  
Kyusik Kim ◽  
Sohee Jeong ◽  
Migyeom Lee ◽  
Tae-il Kim
Keyword(s):  
Gene Delivery ◽  
Reductive Amination ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Buffering Capacity ◽  
Serum Stability ◽  
High Osmolality ◽  
Cox 2 ◽  
Serum Free Conditions ◽  
Positively Charged

In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose and polyethylenimine 2K (PEI2K) were crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR and FTIR. The synthesized SP2K polymers could form positively charged (~40 mV zeta-potential) and nano-sized (150–200 nm) spherical polyplexes with plasmid DNA (pDNA). They showed lower cytotoxicity than PEI25K but concentration-dependent cytotoxicity. Among them, SP2K7 and SP2K10 showed higher transfection efficiency than PEI25K in both serum and serum-free conditions, revealing the good serum stability. It was found that SP2K polymers possessed high osmolality and endosome buffering capacity. The transfection experiments with cellular uptake inhibitors suggest that the transfection of SP2K polymers would progress by multiple pathways, including caveolae-mediated endocytosis. It was also thought that caveolae-mediated endocytosis of SP2K polyplexes would be facilitated through cyclooxygenase-2 (COX-2) expression induced by high osmotic pressure of SP2K polymers. Confocal microscopy results also supported that SP2K polyplexes would be internalized into cells via multiple pathways and escape endosomes efficiently via high osmolality and endosome buffering capacity. These results demonstrate the potential of SP2K polymers for gene delivery systems.

Sign in / Sign up

Export Citation Format

Share Document