Red-blood-cell-mimetic gene delivery systems for long circulation and high transfection efficiency in ECs

2018 ◽  
Vol 6 (37) ◽  
pp. 5975-5985 ◽  
Author(s):  
Xuefang Hao ◽  
Qian Li ◽  
Huaning Wang ◽  
Khan Muhammad ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Blood Cell ◽  
Immune Evasion ◽  
Red Blood Cell ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
High Transfection Efficiency

Red-blood-cell-biomimetic gene delivery systems exhibit long circulation, strong immune evasion and high transfection efficiency.

scholarly journals Highly Osmotic Oxidized Sucrose-Crosslinked Polyethylenimine for Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 87
Author(s):  
Jaehong Park ◽  
Kyusik Kim ◽  
Sohee Jeong ◽  
Migyeom Lee ◽  
Tae-il Kim
Keyword(s):  
Gene Delivery ◽  
Reductive Amination ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Buffering Capacity ◽  
Serum Stability ◽  
High Osmolality ◽  
Cox 2 ◽  
Serum Free Conditions ◽  
Positively Charged

In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose and polyethylenimine 2K (PEI2K) were crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR and FTIR. The synthesized SP2K polymers could form positively charged (~40 mV zeta-potential) and nano-sized (150–200 nm) spherical polyplexes with plasmid DNA (pDNA). They showed lower cytotoxicity than PEI25K but concentration-dependent cytotoxicity. Among them, SP2K7 and SP2K10 showed higher transfection efficiency than PEI25K in both serum and serum-free conditions, revealing the good serum stability. It was found that SP2K polymers possessed high osmolality and endosome buffering capacity. The transfection experiments with cellular uptake inhibitors suggest that the transfection of SP2K polymers would progress by multiple pathways, including caveolae-mediated endocytosis. It was also thought that caveolae-mediated endocytosis of SP2K polyplexes would be facilitated through cyclooxygenase-2 (COX-2) expression induced by high osmotic pressure of SP2K polymers. Confocal microscopy results also supported that SP2K polyplexes would be internalized into cells via multiple pathways and escape endosomes efficiently via high osmolality and endosome buffering capacity. These results demonstrate the potential of SP2K polymers for gene delivery systems.

NON-CONDENSING POLYMERIC GENE DELIVERY SYSTEMS: PRINCIPLES AND APPLICATIONS

Nano LIFE ◽  
2010 ◽  
Vol 01 (03n04) ◽  
pp. 219-237 ◽  
Author(s):  
SHARDOOL JAIN ◽  
HUSAIN ATTARWALA ◽  
MANSOOR AMIJI
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Contemporary Literature ◽  
Delivery Systems ◽  
Expression Of Genes ◽  
Cytoplasmic Dna ◽  
Polymeric Delivery ◽  
Systemic Gene Delivery

Gene therapy holds tremendous promise in prevention and treatment of diseases as the approach is based on regulating the expression of genes that are responsible for pathological conditions. The biggest bottleneck for gene delivery has been the development of safe and efficacious delivery systems. Although non-viral vectors are considered as much safer options than their viral counterparts, they suffer from low transfection efficiency. In this review, we highlight the role of non-condensing polymeric delivery systems for oral and systemic gene delivery. Using evidence from contemporary literature, non-condensing polymeric microparticle and nanoparticle systems afford physical encapsulation of the nucleic acid construct and can be engineered for targeted delivery to tissues and cells. Additionally, these systems have shown less toxicity and afford sustained cytoplasmic DNA delivery for efficient nuclear uptake and transfection for both DNA vaccines and therapeutic genes.

A dendritic catiomer with an MOF motif for the construction of safe and efficient gene delivery systems

2017 ◽  
Vol 5 (42) ◽  
pp. 8322-8329 ◽  
Author(s):  
Shuqi Dong ◽  
Qixian Chen ◽  
Wei Li ◽  
Zhu Jiang ◽  
Jianbiao Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gold Standard ◽  
Transfection Efficiency ◽  
A549 Cells ◽  
Delivery Systems ◽  
The Core ◽  
Efficient Gene

The dendritic catiomer using biocompatible Zr-MOFs as the core exhibited a markedly higher transfection efficiency and lower cytotoxicity than the commercial gold standard branched PEI25k in A549 cells.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

Preparation, characterization and transfection efficiency of cationic PEGylated PLA nanoparticles as gene delivery systems

2007 ◽  
Vol 130 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Jiji Chen ◽  
Buning Tian ◽  
Xiang Yin ◽  
Yanqiong Zhang ◽  
Duosha Hu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Delivery Systems

CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

2019 ◽  
Vol 30 (10) ◽  
pp. 2567-2576
Author(s):  
Xinghong Duo ◽  
Lingchuang Bai ◽  
Jun Wang ◽  
Hao Ji ◽  
Jintang Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Gene Delivery System ◽  
High Transfection Efficiency

Poly(aspartate-g-PEI800), a polyethylenimine analogue of low toxicity and high transfection efficiency for gene delivery

Biomaterials ◽  
2007 ◽  
Vol 28 (32) ◽  
pp. 4889-4900 ◽  
Author(s):  
May P. Xiong ◽  
M. Laird Forrest ◽  
Giangthy Ton ◽  
Anni Zhao ◽  
Neal M. Davies ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
High Transfection Efficiency ◽  
Low Toxicity

AMD3100 Conjugates as Components of Targeted Nonviral Gene Delivery Systems:  Synthesis and in Vitro Transfection Efficiency of CXCR4-Expressing Cells

2004 ◽  
Vol 15 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Bertrand Le Bon ◽  
Nathalie Van Craynest ◽  
Jean-Michel Daoudi ◽  
Christophe Di Giorgio ◽  
Abraham J. Domb ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Nonviral Gene Delivery ◽  
In Vitro Transfection
Sign in / Sign up

Export Citation Format

Share Document