Molecular cloning of zebrafish (Danio rerio) MutS homolog 6(MSH6) and noncoordinate expression of MSH6 gene activity and G-T mismatch binding proteins in zebrafish larvae

2003 ◽  
Vol 297A (2) ◽  
pp. 118-129 ◽  
Author(s):  
Fu-lung Yeh ◽  
Hwei-ling Yan ◽  
Shi-ya Wang ◽  
Ting-yi Jung ◽  
Todd Hsu
Chemosphere ◽  
2019 ◽  
Vol 217 ◽  
pp. 629-635 ◽  
Author(s):  
Jie Gu ◽  
Jiayao Zhang ◽  
Yaoyao Chen ◽  
Hongye Wang ◽  
Min Guo ◽  
...  

2020 ◽  
Vol 188 ◽  
pp. 109870 ◽  
Author(s):  
Jie Gu ◽  
Jiang Wu ◽  
Shuqin Xu ◽  
Liye Zhang ◽  
Deling Fan ◽  
...  

2015 ◽  
Vol 32 (1) ◽  
pp. 7-16 ◽  
Author(s):  
Yongmei Fan ◽  
Qing Feng ◽  
Kehua Lai ◽  
Weikang Huang ◽  
Chenghui Zhang ◽  
...  

2020 ◽  
Vol 31 (3) ◽  
pp. 91-107 ◽  
Author(s):  
Najihah Azman ◽  
Nur Ain Izzati Mohd Zainudin ◽  
Wan Norhamidah Wan Ibrahim

Fumonisin B1 (FB1) is a common mycotoxin produced by Fusarium species particularly F. proliferatum and F. verticillioides. The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB1 based on the amplification of FUM1 gene, to quantify FB1 produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB1 and to determine EC50 toward the larvae of zebrafish (Danio rerio). Fifty isolates of Fusarium species were isolated from different hosts throughout Malaysia. Successful amplification of the FUM1 gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB1 produced by F. proliferatum isolate B2433 was 6677.32 ppm meanwhile F. verticillioides isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB1 on larvae of zebrafish, five concentrations of FB1 (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB1 exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of FUM1 in every isolate can be detected by FUM1 gene analysis and both of the species produced different concentrations of FB1. This is the first report of FB1 produced by Fusarium species gave a significant effect on zebrafish development.


2012 ◽  
Vol 31 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Katiucia Marques Capiotti ◽  
Lidiane Fazenda ◽  
Luiza Reali Nazario ◽  
Fabiano Peres Menezes ◽  
Luiza Wilges Kist ◽  
...  
Keyword(s):  

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110437
Author(s):  
Yeong-Geun Lee ◽  
Youn Hee Nam ◽  
Jung Eun Gwag ◽  
Jung-Hwan Ko ◽  
Kyeong-Hwa Seo ◽  
...  

A simple method to prepare a high-content rutin fraction from Forsythia koreana flowers (HRFK) is described. Rutin, isolated for the first time from the flowers, was identified from spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. As a result of HPLC quantitative analysis, the content of rutin was found to be 80.0 ± 0.02% in HRFK. Our previous study reported that F koreana MeOH extract (FK) significantly recovered alloxan-induced pancreatic islets in zebrafish. However, HRFK as well as rutin exhibited an enhanced anti-diabetic effect compared to FK in our latest experiments. In conclusion, HRFK, as well as rutin and FK, have potential as anti-diabetic agents.


2011 ◽  
Vol 50 (3-4) ◽  
pp. 318-324 ◽  
Author(s):  
Mingxiang Du ◽  
Zhenning Liang ◽  
Xingzhou Xu ◽  
Jianfeng Li ◽  
Hongxin Ai ◽  
...  

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