Morphological and molecular responses in ovaries of Mytilus galloprovincialis collected in two different sites of the Naples Bay

2018 ◽  
Vol 331 (1) ◽  
pp. 52-60 ◽  
Author(s):  
L. Rosati ◽  
M. Agnese ◽  
M. Verderame ◽  
F. Aniello ◽  
M. Venditti ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Molecular Responses ◽  
Naples Bay

scholarly journals Molecular Responses of Mussel Mytilus galloprovincialis Associated to Accumulation and Depuration of Marine Biotoxins Okadaic Acid and Dinophysistoxin-1 Revealed by Shotgun Proteomics

2020 ◽  
Vol 7 ◽  
Author(s):  
José Carlos Martins ◽  
Dany Domínguez-Pérez ◽  
Catarina Azevedo ◽  
Ana Catarina Braga ◽  
Pedro Reis Costa ◽  
...  
Keyword(s):  
Digestive Gland ◽  
Mytilus Galloprovincialis ◽  
Shotgun Proteomics ◽  
Functional Enrichment ◽  
Toxic Substances ◽  
Post Translational Modification ◽  
Response Dynamics ◽  
Molecular Responses ◽  
Time Points ◽  
Cellular Processes

The molecular pathways behind the toxicity of diarrheic shellfish toxins (DSTs) in bivalves have been scarcely studied. Thus, a shotgun proteomics approach was applied in this work to understand bivalves’ molecular responses to the dinoflagellate Prorocentrum lima (1.0 × 106 cells/L). Protein expression along with toxins levels were analyzed in the gills and digestive gland of the mussel Mytilus galloprovincialis during and after exposure to this toxic strain. Results revealed an accumulation of OA and DTX1 only in the digestive gland with maximum amounts attained at the end of uptake phase (day 5; 2819.2 ± 522.2 μg OA/kg and 1107.1 ± 267.9 μg DTX1/kg). At the end of the depuration phase (day 20), 16% and 47% of total OA and DTX1 concentrations remained in the digestive gland tissues, respectively. The shotgun proteomic analyses yielded 3051 proteins in both organs. A total of 56 and 54 differentially expressed proteins (DEPs) were revealed in the digestive gland and gills, respectively. Both organs presented the same response dynamics along the experiment, although with tissue-specific features. The early response (3 days uptake) was characterized by a high number of DEPs, being more marked in gills, in relation to the latter time points (5 days uptake and depuration). Functional enrichment analysis revealed the up-regulation of carboxylic (GO:0046943) and organic acid transmembrane transporter activity (GO:0005342) pathways after 3 days uptake for digestive gland. Matching to these pathways are a group of proteins related to transmembrane transport and response to toxic substances and xenobiotics, namely P-glycoprotein (ABCB11), Sodium-dependent proline transporter (SLC6A7), and Sideroflexin-1 (SFXN1). According to Clusters of Orthologous Groups (GOs) categories, most of the DEPs found for digestive gland in all time-points were related with “cellular processes and signaling” and involving signal transduction mechanisms, cytoskeleton and post-translational modification, protein turnover, chaperone functions. In gills, the early uptake phase was marked by a balance between DEPs related with “cellular processes and signaling” and “metabolism.” Depuration is clearly marked by processes related with “metabolism,” mainly involving secondary metabolites biosynthesis, transport, and catabolism. Proteomic data are available via ProteomeXchange with identifier PXD022293.

Influence of Salicylic Acid application on Oxidative and Molecular Responses and functional properties of Capsicum annuum L. cultivated in greenhouse conditions

2016 ◽  
Author(s):  
Ana Feregrino-Perez ◽  
Sandra Jimenez-Garcia ◽  
Moises Vazquez-Cruz ◽  
Laura Mejia-Teniente ◽  
Ramon Guevara-Gonzalez ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Capsicum Annuum ◽  
Functional Properties ◽  
Strong Influence ◽  
Molecular Responses

Molecular Responses of Cancers by Natural Products: Modifications of Autophagy Revealed by Literature Analysis

2018 ◽  
Vol 23 (5-6) ◽  
pp. 347-370 ◽  
Author(s):  
Andy Wai Kan Yeung ◽  
Amr El-Demerdash ◽  
Ioana Berindan-Neagoe ◽  
Atanas G. Atanasov ◽  
Yuh-Shan Ho
Keyword(s):  
Natural Products ◽  
Literature Analysis ◽  
Molecular Responses
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