Gonadotrophic cells and gonadal sex differentiation in medaka: Characterization of several northern and southern strains

2015 ◽  
Vol 323 (6) ◽  
pp. 392-397 ◽  
Author(s):  
Yoshifumi Horie ◽  
Tohru Kobayashi
Forests ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 202 ◽  
Author(s):  
Qian Bai ◽  
Chenyi Zhu ◽  
Xia Lei ◽  
Tao Cao ◽  
Shuchai Su ◽  
...  

Pistacia chinensis Bunge is widely acknowledged to be dioecious, but rare monoecious individuals have been found. However, the origin of monoecism and the sex differentiation of different sex types remain intriguing questions. Here, sex expressions were explored by identification of sex-associated DNA markers, determination of the sex stability after grafting, and histological characterization of inflorescence bud development using anatomical analysis. The results showed that (1) although polymorphisms among individuals existed, the banding patterns of Polymerase Chain Reaction (PCR) products for different sex types on the same monoecious tree were consistent; (2) the sex expressions of grafted trees were not consistent with those of scions, indicating that monoecism probably did not originate from a stable bud mutation; and (3) both males and females underwent a bisexual period, then the stamen primordia in female buds degenerated into the second round tepals, while the pistil primordia in male buds gradually disappeared. During the sex differentiation phase, female buds were spindle-shaped, while the male buds were full teardrop-shaped, and male buds were bigger than female buds. Taken together, no sex-associated DNA marker was found, sex expressions were unstable after grafting, and the alternative sex organs appeared in the early stage of sex differentiation, suggesting that sex determination occurred during floral development instead of the early vegetative period. These results indicated that the sex expressions may be affected by environmental factors, increasing the understanding of sex determination mechanisms in P. chinensis and other species.


PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e56006 ◽  
Author(s):  
Mariko Forconi ◽  
Adriana Canapa ◽  
Marco Barucca ◽  
Maria A. Biscotti ◽  
Teresa Capriglione ◽  
...  

2014 ◽  
Vol 47 (2) ◽  
pp. 632-639 ◽  
Author(s):  
Bao Lou ◽  
Dongdong Xu ◽  
Zhi Geng ◽  
Yurong Zhang ◽  
Wei Zhan ◽  
...  
Keyword(s):  

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 878F-878 ◽  
Author(s):  
Deepak K. Khandka ◽  
Ali Nejidat ◽  
Moshe Tal ◽  
Avi Golan-Goldhirsh

Several horticulturally important species are dioecious (e.g., pistachio, date palm, poplar, and others). It would be advantageous if the gender of a seedling could be determined at the vegetative stage. In this report, we present results of our search for molecular markers for sex differentiation in dioecious species. The method used was bulked segregant analysis of random amplified polymorphic DNA (RAPD) for sex. A male-specific marker fragment OPB01-1470 was obtained in Mercurialis annua. The sex linkage and characterization of this marker will be discussed.


Gene ◽  
2017 ◽  
Vol 614 ◽  
pp. 56-64 ◽  
Author(s):  
Dong-Rui Li ◽  
Hui-Li Ye ◽  
Jin-Shu Yang ◽  
Fan Yang ◽  
Mo-Ran Wang ◽  
...  

2007 ◽  
Vol 236 (8) ◽  
pp. 2198-2206 ◽  
Author(s):  
Denise Vizziano ◽  
Gwenaëlle Randuineau ◽  
Daniel Baron ◽  
Chantal Cauty ◽  
Yann Guiguen

Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.


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