scholarly journals Zona Pellucida sperm‐binding protein 3 receptor distribution during Gopc −/− globozoospermic spermatogenesis

2021 ◽  
Author(s):  
Maider Bizkarguenaga ◽  
Laura Gomez‐Santos ◽  
Juan Francisco Madrid ◽  
Francisco José Sáez ◽  
Edurne Alonso
Keyword(s):  
Zona Pellucida ◽  
Binding Protein ◽  
Sperm Binding ◽  
Receptor Distribution

Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida

1988 ◽  
Vol 127 (2) ◽  
pp. 287-295 ◽  
Author(s):  
Maurice J. Ringuette ◽  
Margaret E. Chamberlin ◽  
Anne W. Baur ◽  
Donna A. Sobieski ◽  
Jurrien Dean
Keyword(s):  
Molecular Analysis ◽  
Zona Pellucida ◽  
Binding Protein ◽  
Sperm Binding

scholarly journals Integrative analysis reveals pathways associated with sex reversal in Cynoglossus semilaevis

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8801 ◽  
Author(s):  
Zhan Ye ◽  
Weifeng Wang ◽  
Yaqun Zhang ◽  
Liping Wang ◽  
Yu Cui ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zona Pellucida ◽  
Molecular Mechanisms ◽  
Binding Protein ◽  
Sex Reversal ◽  
Cynoglossus Semilaevis ◽  
Integrative Analysis ◽  
Sperm Binding ◽  
Gene And Protein Expression ◽  
Related Transcription Factor

Sex reversal is a complex biological phenomenon exhibited by Cynoglossus semilaevis. Some genetic females may irreversibly convert to pseudomales, thus increasing aquaculture costs because males grow much more slowly than females. In this study, an integrative analysis of transcriptome and proteome was performed to compare differences in gene and protein expression in females and pseudomales after gonad differentiation in C. semilaevis. Based on RNA-Seq results, 1893 genes showed differences in expression at the transcript level between females and pseudomales. Of these differentially expressed genes (DEGs), zona pellucida sperm-binding protein 4-like (LOC103393374 , ZP4), zona pellucida sperm-binding protein 4-like (LOC103396071, ZP4) and forkhead box L2 (foxl2) were highly expressed in females and doublesex and mab-3 related transcription factor 1(dmrt1) and doublesex and mab-3 related transcription factor 3 (dmrt3) were highly expressed in pseudomales. GO enrichment analysis results indicate that wnt signaling pathways and oocyte maturation are two terms enriched in female. At the protein level, Tandem Mass Tags analysis revealed that 324 proteins differed in their relative abundance between pseudomales and females. KEGG analysis found that pseudo-highly expressed proteins were enriched in the ubiquitin mediated proteolysis pathway. For integrative analysis, the Spearman correlation coefficient between the transcriptome and proteome was 0.59. Among 52 related genes, 46 DEGs (88%) were well matched in their levels of change in protein abundance. These findings reveal major active pathways in female and pseudomale gonads after sex reversal and provide new insights into molecular mechanisms associated with sex reversal regulatory network.

Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽  
2000 ◽  
pp. 15-23 ◽  
Author(s):  
K Jewgenow ◽  
M Rohleder ◽  
I Wegner
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Antigenic Determinants ◽  
Vitro Fertilization ◽  
Contraceptive Vaccine ◽  
Sperm Binding ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

scholarly journals Epididymosomes Transfer Epididymal Sperm Binding Protein 1 (ELSPBP1) to Dead Spermatozoa During Epididymal Transit in Bovine1

2012 ◽  
Vol 87 (4) ◽  
Author(s):  
Olivier D'Amours ◽  
Gilles Frenette ◽  
Louis-Jean Bordeleau ◽  
Nancy Allard ◽  
Pierre Leclerc ◽  
...  
Keyword(s):  
Binding Protein ◽  
Epididymal Sperm ◽  
Sperm Binding

Sperm binding to the human zona pellucida and calcium influx in response to GnRH and progesterone

Andrologia ◽  
2002 ◽  
Vol 34 (5) ◽  
pp. 301-307 ◽  
Author(s):  
P. Morales ◽  
E. Pizarro ◽  
M. Kong ◽  
C. Pasten
Keyword(s):  
Zona Pellucida ◽  
Calcium Influx ◽  
Sperm Binding

Characterization of Human Sperm Binding to Homologous Recombinant Zona Pellucida Proteins

2011 ◽  
Vol 18 (9) ◽  
pp. 876-885 ◽  
Author(s):  
Mayel Chirinos ◽  
Cecilia Cariño ◽  
María Elena González-González ◽  
Ernesto Arreola ◽  
Rodrigo Reveles ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Human Sperm ◽  
Sperm Binding
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