Sperm surface hyaluronan binding protein (HABP1) interacts with zona pellucida of water buffalo (Bubalus bubalis) through its clustered mannose residues

2002 ◽  
Vol 64 (2) ◽  
pp. 235-244 ◽  
Author(s):  
Ilora Ghosh ◽  
Kasturi Datta
Keyword(s):  
Zona Pellucida ◽  
Water Buffalo ◽  
Binding Protein ◽  
Bubalus Bubalis ◽  
Sperm Surface ◽  
Hyaluronan Binding

Mannose-binding molecules of rat spermatozoa and sperm–egg interaction

Zygote ◽  
1999 ◽  
Vol 7 (4) ◽  
pp. 335-346 ◽  
Author(s):  
Hiromi Yoshida-komiya ◽  
Daulat Ram P. Tulsiani ◽  
Toshio Hirayama ◽  
Yoshihiko Araki
Keyword(s):  
Zona Pellucida ◽  
Plasma Membranes ◽  
Binding Protein ◽  
Sprague Dawley ◽  
Binding Studies ◽  
Sperm Surface ◽  
Mannose Binding Protein ◽  
Competitive Inhibitors ◽  
Mannose Binding ◽  
Dose Dependent

We have previously reported the occurrence and partial characterisation of an α-D-mannosidase activity on plasma membranes of rat, mouse, hamster and human spermatozoa. A soluble isoform of the rat sperm surface mannosidase was purified and polyclonal antibody raised. Since several reports have suggested that mannosyl residues on the rat, mouse and human zona pellucida may be involved in sperm-zona binding, studies were undertaken to examine the receptor-like role of mannose-binding molecules on rat spermatozoa. Sprague-Dawley rats (25–30-days old) were superovulated and eggs collected from the oviduct were treated with 0.3% hyaluronidase to remove the cumulus cells. Spermatozoa, collected from the cauda epididymis were capacitated for 5 h at 37 °C in 5% CO2 in air. The sperm–zona binding assay was performed in the presence of increasing concentrations of several sugars as well as preimmune and immune (anti-mannosidase or anti-mannose binding protein) IgG. Data from these studies show that: (1) significantly fewer sperm bound per egg in the presence of competitive inhibitors of mannosidase; (2) among the sugars examined, D-mannose was the most potent inhibitor causing 70% reduction in the number of sperm bound per egg; (3) anti-mannosidase or anti-mannose binding protein (but not preimmune) IgG showed a dose-dependent reduction in the number of sperm bound per egg; (4) anti-mannosidase IgG (but not anti-mannose binding protein IgG) showed a dose-dependent inhibition of sperm surface mannosidase activity; (5) the competitive inhibitors of mannosidase or the immune IgG had no effect on sperm motility or the sperm acrosome reaction. These results suggest that mannose-binding molecule(s) such as α-D-mannosidase or mannose-binding protein on the spermatozoa may recognise mannosyl residues on zona pellucida, and play a receptor-like role in sperm-egg interaction in the rat.

scholarly journals Overexpression of hyaluronan-binding protein 1 promotes the proliferation, migration, and invasion of malignant glioma

2020 ◽  
Vol 9 (1) ◽  
pp. 1-11
Author(s):  
Zijun Yang ◽  
Shu Song ◽  
Wenchao Yin ◽  
Xin Qian ◽  
Qiang Yu ◽  
...  
Keyword(s):  
Malignant Glioma ◽  
Binding Protein ◽  
Migration And Invasion ◽  
Hyaluronan Binding

scholarly journals Traumatic Reticuloperitonitis in Water Buffalo (Bubalus bubalis): Clinical Findings and the Associated Inflammatory Response

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Maged El-Ashker ◽  
Mohamed Salama ◽  
Mohamed El-Boshy
Keyword(s):  
Inflammatory Response ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Clinical Findings ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Amyloid A ◽  
Diagnostic Usefulness ◽  
Factor Alpha ◽  
Traumatic Reticuloperitonitis

The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful.

Comparison of feed intake, digestibility and fattening performance of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis)

2004 ◽  
Vol 75 (6) ◽  
pp. 549-555 ◽  
Author(s):  
Rosalina M. LAPITAN ◽  
Arnel N. DEL BARRIO ◽  
Osamu KATSUBE ◽  
Tomomi TOKUDA ◽  
Edgar A. ORDEN ◽  
...  
Keyword(s):  
Feed Intake ◽  
Water Buffalo ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Fattening Performance

scholarly journals Deletion of Hybid (hyaluronan-binding protein involved in hyaluronan depolymerization) results in attenuation of osteoarthritis in mice

2021 ◽  
Author(s):  
Masahiro Momoeda ◽  
Susana de Vega ◽  
Haruka Kaneko ◽  
Chiho Yoshinaga ◽  
Masayuki Shimoda ◽  
...  
Keyword(s):  
Binding Protein ◽  
Hyaluronan Binding

scholarly journals Melanoma maligno em búfalos (Bubalus bubalis) albinos

2015 ◽  
Vol 36 (5) ◽  
pp. 3239 ◽  
Author(s):  
Maria Cecília Florisbal Damé ◽  
Clairton Marcolongo-Pereira ◽  
Letícia Fiss ◽  
Maria de Lourdes Adrién ◽  
Ana Lucia Schild
Keyword(s):  
Skeletal Muscle ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Renal Capsule ◽  
Southern Brazil ◽  
Epithelioid Cells ◽  
Melanocytic Tumors ◽  
Immunohistochemistry Staining ◽  
S 100 ◽  
Collagenous Stroma

<p>Two albino water buffalo affected by multiple melanocytic tumors in Southern Brazil are described. Grossly, there were multiple dark tumors within the skin, skeletal muscle, lungs, and lymph nodes. The tumor was also present in the pericardial sac, renal capsule, mediastinum and pleura. Microscopically, the tumors consisted of polyhedral epithelioid cells arranged in solid nests or interwoven fascicles supported by a thin and sparse collagenous stroma. The cytoplasm was eosinophilic and sometimes contained varying amounts of melanin pigment. The mitotic rates were low. Immunohistochemistry staining with Tyrosinase, Melan-A, Vimentin, S-100 protein, and neurofilament were positive. It is possible that the polymorphisms related to pigmentation in albino buffalo contributed with a high risk of developing melanoma as suggested in humans.</p>

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