Pollen morphology and its taxonomic potential in some selected taxa of Caesalpiniaceae observed under light microscopy and scanning electron microscopy

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

Download Full-text

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽

10.11646/phytotaxa.207.1.9 ◽

2015 ◽

Vol 207 (1) ◽

pp. 135 ◽

Cited By ~ 1

Author(s):

Giovanni Raul Bogota ◽

Carina Hoorn ◽

Wim Star ◽

Rob Langelaan ◽

Hannah Banks ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Transmission Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Pollen Grains ◽

The Other ◽

Transmission Electron ◽

Palm Species ◽

Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera.

Download Full-text

Pollen morphology of the Myrtaceae. Part 3: tribes Chamelaucieae, Leptospermeae and Lindsayomyrteae

Australian Journal of Botany ◽

10.1071/bt11176 ◽

2012 ◽

Vol 60 (3) ◽

pp. 225 ◽

Cited By ~ 9

Author(s):

Andrew H. Thornhill ◽

Peter G. Wilson ◽

Jeff Drudge ◽

Matthew D. Barrett ◽

Geoff S. Hope ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Prolate Spheroidal ◽

Scanning Electron

The pollen morphology of 36 genera and 147 species from the Myrtaceae tribes Chamelaucieae, Leptospermeae and Lindsayomyrteae was surveyed using scanning electron microscopy (SEM) and light microscopy (LM). Syncolpate pollen were observed in all genera of Leptospermeae and some genera of Chamelaucieae. Genera of tribe Chamelaucieae displayed five distinct colpal morphologies, which makes it the tribe with the most diverse pollen in Myrtaceae. Six genera of Chamelaucieae, including Actinodium, Chamelaucium, Darwinia, Homoranthus, Pileanthus and Verticordia, produce large acolpate pollen not observed in any other Myrtaceae. Two of these genera produce distinct pollen; Actinodium is the only genus to have prolate-spheroidal shaped pollen, and Pileanthus pollen is large and dicolporate. A number of anomalous aperture types occurred in species of Chamelaucieae, including monocolporate (Homoranthus thomasii), pentacolporate (Calytrix oldfieldii) and hexacolporate (Sannantha tozerensis). Pollen of Lindsayomyrteae appeared similar to those of Leptospermeae and Chamelaucieae, and on the basis of pollen features, could be related to these two tribes.

Download Full-text

Pollen morphology of the Myrtaceae. Part 1: tribes Eucalypteae, Lophostemoneae, Syncarpieae, Xanthostemoneae and subfamily Psiloxyloideae

Australian Journal of Botany ◽

10.1071/bt11174 ◽

2012 ◽

Vol 60 (3) ◽

pp. 165 ◽

Cited By ~ 15

Author(s):

Andrew H. Thornhill ◽

Geoff S. Hope ◽

Lyn A. Craven ◽

Michael D. Crisp

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Palynological Study ◽

Scanning Electron

A family-wide palynological study of Myrtaceae was conducted using scanning electron microscopy (SEM) and light microscopy (LM). In this part of the study, the pollen morphology of 18 genera and 150 species from the Myrtaceae tribes of subfamily Myrtoideae, Eucalypteae, Lophostemoneae, Syncarpieae, Xanthostemoneae and subfamily Psiloxyloideae are presented. It was found that the most commonly observed pollen in these groups was parasyncolpate with a rugulate exine, whereas some species possessed an apocolpial island. The large, and sometimes syndemicolpate, pollen of Eucalypteae genera Angophora and Corymbia differed from all other genera. Most Eucalyptus pollen had endopores with a thickened exine.

Download Full-text

Pollen morphology of Lagotis (Scrophulariaceae)

Canadian Journal of Botany ◽

10.1139/b95-075 ◽

1995 ◽

Vol 73 (5) ◽

pp. 701-709 ◽

Cited By ~ 3

Author(s):

Charles L. Argue

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Key Words ◽

Pollen Morphology ◽

Pollen Grains ◽

As Species ◽

Close Relationship ◽

Scanning Electron

The pollen grains of 18 species of Lagotis have been examined using light microscopy and scanning electron microscopy of whole and fractured pollen. The grains are characterized by tricolporate apertures with smooth to sparsely granular ectocolpus membranes; lolongate endoapertures with unthickened margins included within the ectocolpi; relatively large exinous processes over the endoaperture; and columellate, semitectate, reticulate to microreticulate exines with or without supramurial processes. Lumina are reduced in size toward the colpus, but the transition is gradual, and a sharply bounded, differentially sculptured colpus border is absent. Lagotis pollen is distinct from that of the Selagineae and Globularia and indistinct from that of some Veroniceae; however, the characters shared with the latter are of wide occurrence in the Scrophulariaceae. Pollen morphology supports the distinction between L. brachystachya and L. stolonifera. It is consistent with the postulated close relationship between L. micrantha and L. integra, and provides no evidence to support the reestablishment of L. cashmeriana, L. minor, L. pallasii, or L. kunawurensis as species distinct from L. glauca. Key words: pollen morphology, Lagotis, Globularia, Selagineae, Veroniceae.

Download Full-text

Pollen Morphological Investigations of Salvia L. In Southeastern of Turkey and Its Taxonomic Implication

Bangladesh Journal of Plant Taxonomy ◽

10.3329/bjpt.v28i2.57135 ◽

2021 ◽

Vol 28 (2) ◽

pp. 395-403

Author(s):

Fatma Mungan Kiliç

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Pollen Grains ◽

Surface Sculpture ◽

Plant Taxon ◽

Pollen Shape ◽

Micromorphological Characters ◽

Scanning Electron

In this study, the pollen morphology and exine structure of nine species of the genus Salvia L. (Lamiaceae) were investigated using light microscopy and scanning electron microscopy (SEM). Six micromorphological characters (pollen shape, polar length, equatorial width, exine and intine thickness, colpus length and colpus width) of pollen grains of Salvia have been identified. The palynological observations revealed that pollen grains of most studied taxa of Salvia were suboblate shape and possess hekzacolpate aperture. Tectal surface sculpture was a good criterion to identify particular taxa from Salvia. The pollen of which is characterized by reticulate, the pollen ornamentation was similar in all studied taxa. Bangladesh J. Plant Taxon. 28(2): 395-403, 2021 (December)

Download Full-text

24A study on pollen morphology of the family Oleaceae Hoffmanns. & Link in Vietnam

Ministry of Science and Technology, Vietnam ◽

10.31276/vjst.63(8).19-24 ◽

2021 ◽

Vol 63 (9) ◽

pp. 19-24

Author(s):

Hong Quang Bui ◽

◽

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Pollen Grains ◽

Morphological Features ◽

Prolate Spheroidal ◽

The Family ◽

Scanning Electron ◽

Sem Analyses

This paper uses scanning electron microscopy (SEM) analyses and light microscopy (LM) to study pollen grains of species of the family Oleaceae Hoffmanns. & Link distributed in Vietnam, including 23 species belonging to 6 genera of the family (Oleaceae). Pollen grains of all species (Oleaceae) in Vietnam are isopolar, radial, 3- prolate pollen grains small size, with two types: subprolate type (Jasminum and Olea) and prolate spheroidal type (Jasminum, Fraxinus, Ligustrum, Osmanthus, and Chengiodendron). The surface of the pollen grains in most species is reticulate. These are important morphological features to classify the Nhai family through pollen morphology

Download Full-text

Pollen morphology of the Myrtaceae. Part 4: tribes Kanieae, Myrteae and Tristanieae

Australian Journal of Botany ◽

10.1071/bt11177 ◽

2012 ◽

Vol 60 (3) ◽

pp. 260 ◽

Cited By ~ 16

Author(s):

Andrew H. Thornhill ◽

Geoff S. Hope ◽

Lyn A. Craven ◽

Michael D. Crisp

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

The Other ◽

Pollen Types ◽

Scanning Electron

Pollen morphology of 44 genera and 101 species from the Myrtaceae tribes Kanieae, Myrteae and Tristanieae was surveyed using scanning electron microscopy (SEM) and light microscopy (LM). Most Myrteae pollen were brevicolpate and granulate, which is unique within Myrtaceae, and these are most likely ancestral characters for this tribe. Two main pollen types were observed in tribe Kanieae, one form being with syncolpate colpi and a distinctive granulate exine, and the other with parasyncolpate colpi and a less ornamented exine. Genera Tristania and Thaleropia of tribe Tristanieae produce the smallest pollen in Myrtaceae, whereas Octamyrtus of tribe Myrteae produces the largest pollen observed in Myrtaceae.

Download Full-text

Morphology of pollen grains of Weigela floribunda (Caprifoliaceae): application in taxonomy

Journal of Native and Alien Plant Studies ◽

10.37555/2707-3114.1.2021.247735 ◽

2021 ◽

pp. 299-304

Author(s):

Z. M. Tsymbalyuk ◽

L. M. Nitsenko ◽

S. L. Mosyakin

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Pollen Morphology ◽

Pollen Grains ◽

Polar View ◽

Equatorial View ◽

First Time ◽

Qualitative Characteristics ◽

Scanning Electron

The aim of this research was to provide detailed quantitative and qualitative characteristics on pollen grains of Weigela floribunda (Siebold & Zucc.) K. Koch (Caprifoliaceae) as applied for taxonomy. Pollen morphology of W. floribunda was studied using both light microscopy and scanning electron microscopy. Pollen grains are 3-porate, rarely 4-porate, suboblate to spheroidal (P/E=0.80–1.05); in equatorial view elliptic or circular, in polar view circular or subcircular; medium- or large-sized (P=42.56–61.18 µm, E=42.56–66.50 µm). Pores are circular with distinct or indistinct margins and distinct annuli. Exine sculpture is echinate-microechinate-nanoechinate. Pollen grains of W. flori- bunda were analyzed for the first time in the present study.

Download Full-text