Preparation and characterization of leukocyte‐ and platelet‐rich fibrin membrane derived from cats' blood

2021 ◽  
Author(s):  
Maíra Sales Castilho ◽  
Sheila Canevese Rahal ◽  
Ramiro das Neves Dias Neto ◽  
Ana Cristina Pereira ◽  
Camila Contin Diniz de Almeida Francia ◽  
...  
Keyword(s):  
Platelet Rich Fibrin ◽  
Fibrin Membrane

390 Characterisation of a Platelet Rich Fibrin Membrane and Formation of an Autologous Fibrin Mesh

2021 ◽  
Vol 108 (Supplement_6) ◽  
Author(s):  
Joshua Burke ◽  
Jack Helliwell ◽  
Mikolaj Kowal ◽  
David Jayne
Keyword(s):  
Three Dimensional ◽  
Clinical Results ◽  
Vascular Endothelial ◽  
Blood Draw ◽  
Platelet Rich Fibrin ◽  
Fibrin Scaffold ◽  
Surgical Sutures ◽  
Endothelial Growth Factor ◽  
Range Of Values ◽  
Fibrin Membrane

Abstract Aim Platelet-rich fibrin (PRF) is a three-dimensional fibrin scaffold with associated platelets and leukocytes which releases high quantities of growth factors over a sustained period of time. PRF has shown promising clinical results in promoting wound healing and tissue regeneration. The aims of this feasibility study were to establish optimal spinning methods for production of PRF, to quantify the production of vascular endothelial growth factor (VEGF) by PRF and to explore new vehicles of clinical PRF delivery. Method Assessment of optimal production involved comparisons between Protocol 1 (EDTA bottle) and Protocol 2 (no additive) at three different centrifugation forces: 400g, 1000g and 1700g. VEGF production was analysed using ELISA with varied incubation periods and PRF plug segments. Novel methods for PRF delivery were explored using surgical sutures and a Zimmer® Skin Graft Mesher. Results Protocol 2 demonstrated shorter average time to blood draw (9.8s compared to 13.6s) and to centrifuge (25.5s compared to 33.1s) with a decreased range of values. All PRF segments exhibited a positive correlation between incubation time and amount of VEGF produced with the bottom segments producing on average more VEGF. A segment of the fibrin plug was successfully secured on a suture and meshed in a 1:1.5 ratio. Conclusions PRF production can be optimised using blood bottles with no additive and high centrifugation forces. VEGF production by PRF peaks at 120 hours with the bottom PRF segment exhibiting the highest rate of production. The first description of a PRF mesh enables new clinical applications.

The effect of platelet-rich fibrin membrane on the repair of perforated tympanic membrane: an experimental study

2017 ◽  
Vol 137 (7) ◽  
pp. 695-699 ◽  
Author(s):  
Nuray Ensari ◽  
Özer Erdem Gür ◽  
Mehmet Türker Öztürk ◽  
Dinç Süren ◽  
Ömer Tarık Selçuk ◽  
...  
Keyword(s):  
Experimental Study ◽  
Tympanic Membrane ◽  
Platelet Rich Fibrin ◽  
Perforated Tympanic Membrane ◽  
Fibrin Membrane

Biological characterization of an injectable platelet-rich fibrin mixture consisting of autologous albumin gel and liquid platelet-rich fibrin (Alb-PRF)

Platelets ◽  
2020 ◽  
pp. 1-8 ◽  
Author(s):  
Masako Fujioka-Kobayashi ◽  
Benoit Schaller ◽  
Carlos Fernando De Almeida Barros Mourão ◽  
Yufeng Zhang ◽  
Anton Sculean ◽  
...  
Keyword(s):  
Biological Characterization ◽  
Platelet Rich Fibrin

Urgent Therapeutic Grafting of Platelet-Rich Fibrin Membrane in Descemetocele

Cornea ◽  
2016 ◽  
Vol 35 (9) ◽  
pp. 1245-1249 ◽  
Author(s):  
Mehmet Erol Can ◽  
Gamze Dereli Can ◽  
Nurullah Cagil ◽  
Hasan Basri Cakmak ◽  
Nuran Sungu
Keyword(s):  
Platelet Rich Fibrin ◽  
Fibrin Membrane

Simultaneous Implant Placement and Guided Bone Regeneration Using a Platelet-Rich Fibrin Membrane: Case Report

2017 ◽  
Vol 7 (1) ◽  
pp. 19-24
Author(s):  
Yung-Ting Hsu ◽  
Yu-Hsiang Chou ◽  
Tzu-Hsuan Yang ◽  
Yi-Min Wu ◽  
Chiung-Lin Huang ◽  
...  
Keyword(s):  
Case Report ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Platelet Rich Fibrin ◽  
Implant Placement ◽  
Fibrin Membrane
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