Enamel demineralization model in primary teeth: Micro‐CT and SEM assessments of artificial incipient lesion

Author(s):  
Arlete González‐Sotelo ◽  
Rosalía Contreras‐Bulnes ◽  
Laura E. Rodríguez‐Vilchis ◽  
Maria de los Angeles Moyaho‐Bernal ◽  
Efraín Rubio‐Rosas ◽  
...  
Author(s):  
J. C. Barasuol ◽  
M. P. Alcalde ◽  
E. A. Bortoluzzi ◽  
M. A. H. Duarte ◽  
M. Cardoso ◽  
...  

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
R. A. Valério ◽  
C. T. Rocha ◽  
R. Galo ◽  
M. C. Borsatto ◽  
M. C. P. Saraiva ◽  
...  

This study evaluated the effect of CO2laser irradiation and topical fluoride therapy in the control of caries progression on primary teeth enamel. 30 fragments (3×3×2 mm) from primary canines were submitted to an initial cariogenic challenge that consisted of immersion on demineralizing solution for 3 hours and remineralizing solution for 21 hours for 5 days. Fragments were randomly assigned into three groups (n=10): L: CO2laser (λ=10.6 μm), APF: 1.23% acidulated phosphate fluoride, and C: no treatment (control). CO2laser was applied with 0.5 W power and 0.44 J/cm2energy density. Fluoride application was performed with 0.1 g for 1 minute. Cariogenic challenge was conducted for 5 days following protocol previously described. Subsurface Knoop microhardness was measured at 30 μm from the edge. Obtained data were subjected to analysis the variance (ANOVA) and Duncan test with significance of 5%. It was found that the L group showed greater control of deciduous enamel demineralization and were similar to those of APF group, while being statistically different from C group (P≤0.05) that showed the lowest microhardness values. It was concluded that CO2laser can be an additional resource in caries control progression on primary teeth enamel.


2016 ◽  
Vol 40 (6) ◽  
pp. 496-502 ◽  
Author(s):  
S Hayashi-Sakai ◽  
N Numa-Kinjoh ◽  
M Sakamoto ◽  
J Sakai ◽  
J Matsuyama ◽  
...  

Objective: Most cases of hypophosphatasia (HPP) exhibit early loss of primary teeth. Results of micro-computed tomography (micro-CT) analysis of teeth with HPP have not yet been reported. The purpose of the present study was to describe the size and mineral density distribution and mapping of exfoliated teeth with HPP using micro CT. Study design: Seven exfoliated teeth were obtained from a patient with HPP. Exfoliated teeth sizes were measured on micro CT images and mineral densities of the mandibular primary central incisors were determined. Results: Partial dentures were fabricated for the patient to replace the eight primary teeth which had exfoliated. Most primary teeth sizes were within the normal range. The mean values of enamel and dentin mineral densities in teeth with HPP were 1.35 and 0.88 g/cm3, respectively, in the mandibular primary central incisors. Conclusion: Mineral density distribution and mapping revealed that the values in teeth with HPP were lower than the homonymous teeth controls in all regions from the crown to apex. Furthermore, it was demonstrated that the differences between HPP and controls were larger on the crown side and the differences tended to converge on the apex side. These results suggested that the present patient showed mild hypomineralization in the primary dentition.


2017 ◽  
Vol 87 (6) ◽  
pp. 841-846 ◽  
Author(s):  
Aslihan Zeynep Oz ◽  
Abdullah Alper Oz ◽  
Sabahat Yazıcıoglu

ABSTRACT Objectives: The aim of this in vivo study was to investigate the preventive effect of two different adhesives on enamel demineralization and compare these adhesives with a conventional one. Materials and Methods: Fifteen patients requiring the extraction of their first four premolars for orthodontic treatment were included in the study. One premolar was randomly selected, and an antibacterial monomer-containing and fluoride-releasing adhesive (Clearfil Protect Bond, Kuraray Medical, Okayama, Japan) was used for orthodontic bracket bonding. Another premolar was randomly selected, and a fluoride-releasing and recharging orthodontic adhesive (Opal Seal, Ultradent Products, South Jordan, Utah) was used. One premolar was assigned as a control, and a conventional adhesive (Transbond XT, 3M Unitek, Monrovia, Calif) was used. The teeth were extracted after 8 weeks, and the demineralization areas of the 45 extracted teeth were analyzed using microcomputed tomography with software. Results: There was no significant difference between the white spot lesion (WSL) rates of the adhesives (P > .05). The volumes of the WSLs varied from 0 to 0.019349 mm3. Although Opal Seal showed the smallest lesion volumes, there was no significant difference in volumetric measurements of the lesions among the groups (P > .05). Conclusions: The findings indicated no significant differences between the preventive effects of the adhesives used in this in vivo study over 8 weeks.


2019 ◽  
Vol 18 ◽  
pp. e191649
Author(s):  
Guilherme Fantini Ferreira ◽  
Larissa Pereira Lagos de Melo ◽  
Mariana Rocha Nadaes ◽  
Fernanda Maria Mazoni Reis ◽  
Fernanda Miori Pascon ◽  
...  

Aim: To evaluate the performance of three digital radiographic systems in the analysis of root canal filling quality using different intracanal materials for primary teeth. Methods: Twenty-five bovine teeth were divided into 5 groups: Calen® combined with iodoform; Calen® combined with zinc oxide; zinc oxide and eugenol; UltraCal®XS, and 2% chlorhexidine combined with Ca(OH)2 + zinc oxide. Periapical radiographs were obtained with the VistaScan, Express, and SnapShot systems. The quality of the images was evaluated objectively (radiopacity) and subjectively (apical sealing and filling homogeneity). As the reference standard, the teeth were scanned with a micro-CT device. Results: Radiopacity differed among the radiographic systems and materials tested. In general, the greatest difference was observed between the Express and VistaScan systems; Calen® combined with iodoform resulted in the highest radiopacity. The radiographic systems did not differ in terms of homogeneity. However, Calen® combined with iodoform differed from the other materials and exhibited the best results. Regarding apical sealing, the SnapShot system and Calen® combined with zinc oxide provided the best results. Conclusion: Direct digital systems show better performance in evaluating the quality of endodontic treatment in primary teeth and should be preferred for this purpose.


2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
A. A. Al Tuwirqi ◽  
E. A. El Ashiry ◽  
A. Y. Alzahrani ◽  
N. Bamashmous ◽  
T. A. Bakhsh

Objectives. To evaluate the internal adaptation of recent pulp capping materials (TheraCal and Biodentine) relative to MTA when used as indirect pulp capping for primary teeth. Materials and Methods. Thirty primary molars were randomly allocated into three groups, group (A) was TheraCal, group (B) was Biodentine, and MTA was the control group (C). A standardized round class-V cavity (1.5 mm diameter and 2 mm depth) was prepared using a milling machine on the buccal surface of each tooth with the pulpal floor located on the dentin. Then, pulp-capping materials were applied. Finally, all teeth were restored by composite restoration. The internal adaptation of the pulp-capping materials to the dentinal surface was investigated by microcomputed tomography (Micro-CT) to determine the internal gap volume, and by optical coherence tomography (OCT) to determine the high-intensity reflection of light from the floor. Results. Based on Micro-CT findings, TheraCal showed significantly higher internal gap volume than both MTA and Biodentine ( p < 0.001 ), while MTA and Biodentine did not show a significant difference in the gap volume. Based on the OCT findings, TheraCal showed a significantly higher intensity of light reflection than both MTA and Biodentine ( p < 0.001 ); however, there was no significant difference between MTA and Biodentine. Pearson’s correlation test showed that there was a strong positive correlation between Micro-CT and OCT ( r = 0.686 , N = 30 , p < 0.001 ). Conclusions. Biodentine and MTA showed a comparable result in terms of their internal adaptation on the dentinal surface of the primary teeth, and both were better than TheraCal. There is a moderate to a strong positive correlation between Micro-CT and OCT in the measurement of internal adaptation of the tested pulp capping materials. OCT can be helpful and beneficial for the clinical setting and allow dentists to screen and evaluate restorations during follow-up.


2020 ◽  
Vol 23 (3) ◽  
pp. 191-199
Author(s):  
Cansu KOÇYİĞİT ◽  
Burcu Nihan YÜKSEL ◽  
Nurhan ÖZALP
Keyword(s):  
Micro Ct ◽  

2021 ◽  
Vol 48 (3) ◽  
pp. 352-358
Author(s):  
Myunghwan Kim ◽  
Howon Park ◽  
Juhyun Lee ◽  
Hyunwoo Seo

Molar-incisor malformation (MIM) is characterized by malformation in the root with a normal crown. While MIM mostly occurs in the permanent first molar, it has also been reported in the maxillary central incisor and the primary second molar (PSM), but anatomical analysis of the primary teeth with MIM has not been studied to date. In this case report, a patient with MIM was reported, and an extracted PSM with MIM was analyzed with micro computed tomography (CT). A cervical constriction morphology of the cementoenamel junction (CEJ) can be observed in extracted PSM. In micro CT analysis, characteristics such a mineralized plate (cervical mineralized diaphragm) in the CEJ area, complex root canal morphologies, a calcified mass inside the pulp chamber, and constricted pulp chamber of crown portion were observed.


2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Sachiko Hayashi-Sakai ◽  
Takafumi Hayashi ◽  
Makoto Sakamoto ◽  
Jun Sakai ◽  
Junko Shimomura-Kuroki ◽  
...  

Most cases of hypophosphatasia (HPP) exhibit early loss of primary teeth. Results of microcomputed tomography (micro-CT) analysis of teeth with HPP have rarely been reported. The purpose of the present study was to describe the mineral density distribution and mapping of exfoliated teeth from an HPP patient using micro-CT. Four exfoliated teeth were obtained from a patient with HPP. Enamel and dentin mineral densities of exfoliated teeth were measured on micro-CT. The mean values of enamel and dentin mineral densities in mandibular primary central incisors with HPP were 1.61 and 0.98 g/cm3, respectively. The corresponding values in the mandibular primary lateral incisors were 1.60 and 0.98 g/cm3, respectively. Enamel hypoplasia was seen in the remaining teeth, both maxillary and mandibular primary canines and first and second molars. Micro-CT enables nondestructive, noninvasive evaluation and is useful for studying human hard tissues obtained from patients.


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