Fine structure of normal and regenerated shell of Helix

1971 ◽  
Vol 49 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A. S. M. Saleuddin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Organic Matrix ◽  
Nacreous Layer ◽  
Replica Technique ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Inorganic Components ◽  
Scanning Electron

Fine structure of the normal and the regenerated shell of Helix has been studied by thin sectioning, replica technique, and scanning electron microscopy. Normal shell consists of four calcareous layers: innermost nacreous, two cross lamellar, and outermost prismatic. Crystals of the shell are well defined and are surrounded by intercrystalline organic matrix. Intracrystalline organic matrix is recognized, particularly in decalcified sections. Interrelationships between the organic and inorganic components have been studied in decalcified thin sections. Regenerated shell appears similar to nacreous layer of the normal shell. Crystals are large and stacked like bricks. Intracrystalline organic matrix is very prominent. Electron diffraction of the crystals of the regenerated shell generally gives calcite pattern whereas the normal shell gives aragonite. Surface topography of the normal and regenerated shell has been compared by replica techniques.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

Ultrastructure of the soil fungus, Geomyces pannorus

1984 ◽  
Vol 42 ◽  
pp. 738-739
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Low Temperatures ◽  
Soil Fungus ◽  
Organic Soils ◽  
Anatomical Studies ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

scholarly journals Improved imaging and analysis of chlorite in reservoirs and modern day analogues: new insights for reservoir quality and provenance

2018 ◽  
Vol 484 (1) ◽  
pp. 189-204 ◽  
Author(s):  
R. H. Worden ◽  
James E. P. Utley ◽  
Alan R. Butcher ◽  
J. Griffiths ◽  
L. J. Wooldridge ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Analysis Data ◽  
Reservoir Quality ◽  
Phase Identification ◽  
Thin Sections ◽  
Analytical Technique ◽  
Quantitative Mineralogy ◽  
Digital Format ◽  
Scanning Electron

AbstractChlorite is a key mineral in the control of reservoir quality in many siliciclastic rocks. In deeply buried reservoirs, chlorite coats on sand grains prevent the growth of quartz cements and lead to anomalously good reservoir quality. By contrast, an excess of chlorite – for example, in clay-rich siltstone and sandstone – leads to blocked pore throats and very low permeability. Determining which compositional type is present, how it occurs spatially, and quantifying the many and varied habits of chlorite that are of commercial importance remains a challenge. With the advent of automated techniques based on scanning electron microscopy (SEM), it is possible to provide instant phase identification and mapping of entire thin sections of rock. The resulting quantitative mineralogy and rock fabric data can be compared with well logs and core analysis data. We present here a completely novel Quantitative Evaluation of Minerals by SCANning electron microscopy (QEMSCAN®) SEM–energy-dispersive spectrometry (EDS) methodology to differentiate, quantify and image 11 different compositional types of chlorite based on Fe : Mg ratios using thin sections of rocks and grain mounts of cuttings or loose sediment. No other analytical technique, or combination of techniques, is capable of easily quantifying and imaging different compositional types of chlorite. Here we present examples of chlorite from seven different geological settings analysed using QEMSCAN® SEM–EDS. By illustrating the reliability of identification under automated analysis, and the ability to capture realistic textures in a fully digital format, we can clearly visualize the various forms of chlorite. This new approach has led to the creation of a digital chlorite library, in which we have co-registered optical and SEM-based images, and validated the mineral identification with complimentary techniques such as X-ray diffraction. This new methodology will be of interest and use to all those concerned with the identification and formation of chlorite in sandstones and the effects that diagenetic chlorite growth may have had on reservoir quality. The same approach may be adopted for other minerals (e.g. carbonates) with major element compositional variability that may influence the porosity and permeability of sandstone reservoirs.

scholarly journals PROVENANCE OF PINDOS FORELAND FLYSCH DEPOSITS USING SCANNING ELECTRON MICROSCOPY AND MICROANALYSIS

2004 ◽  
Vol 36 (1) ◽  
pp. 607 ◽  
Author(s):  
I. Vakalas ◽  
G. Ananiadis ◽  
A. Zelilidis ◽  
N. Kontopoulos ◽  
B. Tsikouras
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cross Sections ◽  
Thin Sections ◽  
Source Areas ◽  
Probable Source ◽  
Pelagonian Zone ◽  
Source Materials ◽  
Scanning Electron

A number of polished thin sections from two cross sections within the Pindos foreland deposits were petrographically examined while microanalyses on certain minerals were carried out. Chemistry of these minerals is compared to analogous phases occurring in several formations in the neighbourhood of the studied areas which can stand as source areas. Our results reveal that the most probable source materials include the Pindos, Koziakas (and probably and Vourinos) ophiolite complexes, as well as metamorphic sequences of the Pelagonian Zone

Environmental Scanning Electron Microscopy Technique to Identify Asbestos Phases Inside Ferruginous Bodies

2013 ◽  
Vol 19 (2) ◽  
pp. 420-424 ◽  
Author(s):  
Alessandro Croce ◽  
Maya Musa ◽  
Mario Allegrina ◽  
Paolo Trivero ◽  
Caterina Rinaudo
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chemical Elements ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Thin Sections ◽  
The Core ◽  
Microscopy Technique ◽  
Ferruginous Bodies ◽  
Scanning Electron

AbstractFerruginous bodies observed in lungs of patients affected by mesothelioma, asbestosis, and pulmonary carcinoma are important to relate the illness to exposure, environmental or occupational, to asbestos. Identification of the inorganic phase constituting the core of the ferruginous bodies, formed around asbestos but also around phases different from asbestos, is essential for legal purposes. Environmental scanning electron microscopy/energy dispersive spectroscopy was used to identify the fibrous mineral phase in the core of ferruginous bodies observed directly in thin sections of tissue, without digestion of the biological matrix. Spectra were taken with sequential analyses along a line crossing the core of the ferruginous bodies. By comparing the spectra taken near to and far from the core, the chemical elements that make up the core could be identified.

Ultrastructure of the Tertiary Envelope of the Cyst of the Tadpole Shrimp Triops longicaudatus (Branchiopoda; Notostraca)

2000 ◽  
Vol 6 (S2) ◽  
pp. 872-873
Author(s):  
James R. Rosowski ◽  
Terry L. Bartels ◽  
James F. Colburn ◽  
Jannell L. Colton ◽  
Denton Belk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fairy Shrimp ◽  
Distilled Water ◽  
Thin Sections ◽  
Rainfall Events ◽  
Transmission Electron ◽  
Tadpole Shrimp ◽  
Species Specific ◽  
Scanning Electron

Tadpole shrimp inhabit temporary freshwater pools and ponds where their occurrence is largely regulated by rainfall events and water temperature. When dry basins are flooded, cysts of Triops imbibe water and hatch to produce rapidly growing, carapaced larvae. While previous studies show anostracan (fairy shrimp) cyst-surface morphology often species specific, few studies illustrate shell ultrastructure of Triops and none has considered T. longicaudatus. Here we examine the shell of T. longicaudatus (Notostraca) and compare its fine structure to other species of Triops and to that of Artemiafranciscana(Anostraca), which we previously studied.Cysts, produced in culture from Utah broodstock, were purchased from Triops, Inc., 1924 Creighton Rd., Pensacola, FL 32504. Thin sections of cysts were prepared for transmission electron microscopy (TEM) as previously described (Fig. 1). Cysts were also examined with scanning electron microscopy (SEM), dry, whole or fractured (Figs. 2,3), or after imbibition and/or hatching in oxygen saturated, double-distilled water, at 25 ° C.

Pollen morphology of Ginkgo biloba and Cycas revoluta

1986 ◽  
Vol 64 (12) ◽  
pp. 3075-3078 ◽  
Author(s):  
N. Sahashi ◽  
J. Ueno
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ginkgo Biloba ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Spherical Form ◽  
Thin Sections ◽  
Morphological Studies ◽  
Cycas Revoluta ◽  
Scanning Electron

Morphological studies on pollen grains of Ginkgo biloba L. and Cycas revoluta Thunb. were carried out by scanning electron microscopy. The pollen grains of both species are generally oblong with 1-sulcate apertures which are shrunken as a result of dryness. However, the swollen grains show an almost spherical form with a large and rounded germinal aperture. This aperture may not correspond to any aperture type so far known, although the term "anaporate" can be fitted to the swollen pollen grains. Auricular projections, which may be derived from protrusions of the ectosexine, can be seen sometimes on the surface of the pollen grains. These projections remind us of degraded versions of the bladders that may have been present on the pollen grains of the fossil ancestor. The inner side of the exine, which can be seen in thin sections obtained with the freezing microtome, is ornamented with reticulumlike sculptures. These endosculptures may be the first reported among gymnosperm pollen grains.

scholarly journals Improving the Resolution of Sputter-coated Films

2000 ◽  
Vol 8 (2) ◽  
pp. 16-17
Author(s):  
Mary Mager
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
High Resolution ◽  
Carbon Film ◽  
Metal Films ◽  
Pure Gold ◽  
High Magnification ◽  
Conducting Film ◽  
Scanning Electron

After an inquiry from the Microscopy Listserver, I went back to my 1980 copy of Scanning Electron Microscopy, volume I. Several authors had investigated the structure of thin metal films by depositing the films onto carbon-film-covered TEM grids and imaging the films at high magnification. There were several proposals for new devices that have since become standards for high-resolution coaters, but the Listserver inquiry was for a fine conducting film suitabie for high-resolution SEM from an existing sputter coater.There were several factors studied that influenced the fine structure of the films. The first was the materials sputtered: for a given set of conditions of voltage, current and time, platinum gave the finest film, 60% gold-40% palladium (Au/Pd) the next finest and pure gold the least fine.

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