Production of Vitamin B2 (Riboflavin) by Bacillus subtilis

2021 ◽  
Author(s):  
Ronghao Chu ◽  
Rui Li ◽  
Chen Wang ◽  
Rui Ban
Keyword(s):  
Bacillus Subtilis ◽  
Vitamin B2

scholarly journals Safety of vitamin B2 (80%) as riboflavin produced by Bacillus subtilis KCCM‐10445 for all animal species

EFSA Journal ◽  
2018 ◽  
Vol 16 (3) ◽  
Author(s):  
◽  
Guido Rychen ◽  
Gabriele Aquilina ◽  
Giovanna Azimonti ◽  
Vasileios Bampidis ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Animal Species ◽  
Vitamin B2

scholarly journals Studying the effect of potato products in extending the period of potato storage

2021 ◽  
Vol 258 ◽  
pp. 04021
Author(s):  
Nasiba Ibragimova ◽  
Sayyora Murodova ◽  
Denis Otanazarov ◽  
Mutabar Khojanazarova
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Stutzeri ◽  
Saline Soils ◽  
Vitamin B ◽  
Potato Storage ◽  
Vitamin B2 ◽  
Climate Conditions ◽  
And Storage ◽  
Potato Products ◽  
Different Levels

In this research, the cultivation and storage of potatoes grown in saline soils of Khorezm region at different levels with biopreparations, such as Bist (Pseudomonas putida) and Zamin-M (Bacillus subtilis, Bacillus megaterium and Pseudomonas stutzeri), were studied. In the experimental variants, there was 1.381 mg/kg of vitamin C, followed by 0.112 mg/kg of vitamin B6, 0.089 mg/kg of vitamin PP, 0.481 mg/kg of vitamin B12 and 0.092 mg/kg of vitamin B2 when Zamin-M was applied. Potatoes, which were treated with Zamin-M, contained 0.45% Fructose, 0.87% Glucose, 0.92% Sucrose, 0.04% Maltose, and it was higher than other biopreparations. During storage, the number of diseased nodules was 21.8% when treated with Earth ointment, it was 20% when treated with Bist, and it was 19.8% when treated with Zamin-M. The results showed that Zamin-M was suitable in soil and climate conditions of Khorezm region, especially for storage of potato, and it was found that Zamin-M contained microorganism, which activate the synthesis of phytohormones operating under stress. In comparison, the tested Zamin-M extended the shelf life of potatoes by 25% than the control variants.

scholarly journals Genome Sequence of EU-Unauthorized Genetically Modified Bacillus subtilis Strain 2014-3557 Overproducing Riboflavin, Isolated from a Vitamin B2 80% Feed Additive

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Elodie Barbau-Piednoir ◽  
Sigrid C. J. De Keersmaecker ◽  
Véronique Wuyts ◽  
Céline Gau ◽  
Walter Pirovano ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Genome Sequence ◽  
Genetically Modified ◽  
Feed Additive ◽  
Subtilis Strain ◽  
Vitamin B2

INFLUENCE OF BIOGENIC SUBSTANCES ADDING INTO MOLASSES SOLUTIONS ON THE EFFICIENCY OF BIOLOGICALLY ACTIVE SUBSTANCES SYNTHESIS

2021 ◽  
Author(s):  
С.О. СЕМЕНИХИН ◽  
О.В. ВОРОБЬЕВА ◽  
М.В. БАБАКИНА ◽  
В.О. ГОРОДЕЦКИЙ
Keyword(s):  
Bacillus Subtilis ◽  
Rhodotorula Glutinis ◽  
Biologically Active ◽  
Specific Production ◽  
Vitamin B2 ◽  
Cryptococcus Curvatus ◽  
Vital Activity ◽  
Beet Molasses ◽  
Biogenic Substances ◽  
Active Substances

Побочный продукт сахарного производства – свекловичная меласса используется в микробиологической промышленности в качестве компонента высокопродуктивного субстрата для культивирования микроорганизмов, синтезирующих биологически активные вещества. На наш взгляд, перспективна предварительная подготовка мелассы путем ее насыщения биогенными веществами, повышающими эффективность жизнедеятельности микроорганизмов. Для подтверждения этого исследовано влияние внесения биогенных веществ в растворы свекловичной мелассы при их обработке на жизнедеятельность микроорганизмов, способствующих повышению синтеза витамина В2 и жиров. Объектами исследования были раствор свекловичной мелассы с массовой долей сухих веществ 78% и 3 штамма микроорганизмов – Bacillus subtilis B-501, Cryptococcus curvatus Y-2236 и Rhodotorula glutinis Y-332. Анализ исходного содержания макро- и микроэлементов в растворе свекловичной мелассы позволил установить, что содержащихся в мелассе биогенных веществ достаточно для жизнедеятельности Bacillus subtilis B-501 с удельной выработкой витамина В2 в количестве 0,019 г/г сахаров при степени утилизации сахаров 42,55%. Для повышения эффективности жизнедеятельности Rhodotorula glutinis Y-332 требуется внесение сульфатов железа FeSO4 и марганца MnSO4, которые способствуют увеличению удельной выработки жиров с 0,019 до 0,030 г/г сахара в каждом случае. Для повышения эффективности жизнедеятельности Cryptococcus curvatus Y-2236 требуется внесение сульфатов железа FeSO4 и марганца MnSO4, а также источников фосфора KH2PO4 и азота (NH4)2SO4. Внесение указанных биогенных веществ способствует повышению удельной выработки жиров с 0,011 до 0,015–0,020 г/г сахаров. A byproduct of sugar production – beet molasses is used in the microbiological industry as a component of a highly productive substrate for the cultivation of microorganisms that synthesize biologically active substances. In our opinion, the preliminary preparation of molasses by its saturation with biogenic substances to increase the efficiency of the vital activity of microorganisms is promising. To confirm this, the effect of introducing biogenic substances into the solution of beet molasses during their processing on the vital activity of microorganisms that contribute to the increase in the synthesis of vitamin B2 and fats has been studied. The objects of the study were a solution of beet molasses with a mass fraction of dry substances of 78% and 3 strains of microorganisms – Bacillus subtilis B-501, Cryptococcus curvatus Y-2236 and Rhodotorula glutinis Y-332. Analysis of the initial content of macro-and microelements in the solution of beet molasses allowed us to establish that the nutrients contained in the molasses are sufficient for the vital activity of Bacillus subtilis B-501 with a specific production of vitamin B2 in the amount of 0,019 g/g of sugars with a degree of sugar utilization of 42,55%. To improve the vital efficiency of the Rhodotorula glutinis Y-332, the addition of ferrous FeSO4 and manganese MnSO4 sulfates is required, which increase the fats specific production from 0,019 to 0,030 g/g of sugars in each case. To improve the vital efficiency of the Cryptococcus curvatus Y-2236 the addition of ferrous FeSO4 and manganese MnSO4 sulfates, as well as sources of phosphorus KH2PO4 and nitrogenium (NH4)2SO4is required. Adding these nutrients contributes the increase of fats specific production from 0,011 to 0,015–0,020 g/g of sugars.

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Vitamin B2 may help treat sepsis

Nature ◽  
2004 ◽  
Author(s):  
HelenR. Pilcher
Keyword(s):  
Vitamin B2
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