Submicron magnetite-enhanced tribromophenol removal and methanogenesis under microaerobic condition

2018 ◽  
Vol 94 (3) ◽  
pp. 730-738 ◽  
Author(s):  
Fan Yang ◽  
Jing Wang ◽  
Chen Gu ◽  
Ying Han ◽  
Guang-fei Liu ◽  
...  
2016 ◽  
Vol 75 (5) ◽  
pp. 1118-1127 ◽  
Author(s):  
B. Umamaheswari ◽  
K. Priya ◽  
Rama Rajaram

Synthetic fatliquors are useful as a fatliquoring agent, flotation agent and emulsifying agent in a wide range of industrial applications such as leather, pharmacy and farm chemicals. These fatliquors remain recalcitrant to natural biota in existing treatment plants. In the present study, the isolated microaerophilic Serratia sp. HA1 strain CSMB3 is capable of utilizing structurally different fatliquors as the sole substrate for their growth under microaerobic conditions. Degradation of vegetable fatliquors was observed from 95 to 97% in terms of lipids, with the production of lipase at 72 h. Degradation of synthetic fatliquors was observed in terms of chemical oxygen demand from 85% to a minimum of 25%. It is in the order of sulfited/sulfated fatliquors > sulfochlorinated fatliquors > chlorinated fatliquors. A thin layer chromatography chromatogram confirmed the degradation of non polar fatliquor to polar compounds. Production of the red pigment prodigiosin in synthetic fatliquors enhanced the growth of the isolate. Fourier transform infrared spectroscopy (FTIR) confirmed the bioremediation of sulfochlorinated fatliquor into lipids and fatty acids and gas chromatography–mass spectrometry (GC-MS) results confirmed that alcohols and esters are the final end products. Thus the isolated strain CSMB3 may be used in the treatment of wastewaters containing vegetable and synthetic fatliquors.


2004 ◽  
Vol 54 (6) ◽  
pp. 2297-2300 ◽  
Author(s):  
Yumiko Kodama ◽  
Kazuya Watanabe

A facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium, strain YK-1T, was isolated from an underground crude-oil storage cavity at Kuji in Iwate, Japan. The cells were motile, curved rods and had a single polar flagellum. Optimum growth occurred in a low-strength salt medium at pH 7·0 and 25 °C. It utilized sulfide, elemental sulfur, thiosulfate and hydrogen as the electron donors and nitrate as the electron acceptor under anaerobic conditions, but it did not use nitrite. Oxygen also served as the electron acceptor under the microaerobic condition (O2 in the head space 1 %). It did not grow on sugars, organic acids or hydrocarbons as carbon and energy sources. The DNA G+C content of strain YK-1T was 45 mol%. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that its closest relative was Thiomicrospira denitrificans in the ‘Epsilonproteobacteria’, albeit with low homology (90 %). On the basis of physiological and phylogenetic data, strain YK-1T should be classified into a novel genus and species, for which the name Sulfuricurvum kujiense gen. nov., sp. nov. is proposed. The type strain is YK-1T (=JCM 11577T=MBIC 06352T=ATCC BAA-921T).


1986 ◽  
Vol 8 (7) ◽  
pp. 491-496 ◽  
Author(s):  
N. Noparatnaraporn ◽  
K. Sasaki ◽  
Y. Nishizawa ◽  
S. Nagai

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Prasant Kumar ◽  
Ayush V. Ranawade ◽  
Naresh G. Kumar

The present study describes the beneficial effects of potential probioticE. coli16 (pUC8:16gfp) expressingVitreoscillahemoglobin (vgb) gene, associated with bacterial respiration under microaerobic condition, on gastrointestinal (GI) colonization and its antioxidant activity on carbon tetrachloride (CCl4) induced toxicity in Charles Foster rats.In vitro, catalase activity inE. coli16 (pUC8:16gfp) was 1.8 times higher compared toE. coli16 (pUC-gfp) control.In vivo,E. coli16 (pUC8:16gfp) not only was recovered in the fecal matter after 70 days of oral administration but also retained antibacterial activities, whereasE. coli16 (pUC-gfp) was not detected. Oral administration of 200 and 500 μL/kg body weight of CCl4to rats at weekly interval resulted in elevated serum glutamyl pyruvate transaminase (SGPT) and serum glutamyl oxalacetate transaminase (SGOT) levels compared to controls. Rats prefed withE. coli16 (pUC8:16gfp) demonstrated near to normal levels for SGPT and SGOT, whereas the liver homogenate catalase activity was significantly increased compared to CCl4treated rats. Thus, pUC8:16gfpplasmid encodingvgbimproved the growth and GI tract colonization ofE. coli16. In addition, it also enhanced catalase activity in rats harboringE. coli16 (pUC8:16gfp), thereby preventing the absorption of CCl4to GI tract.


2021 ◽  
Author(s):  
Yumin Zhang ◽  
Hector Alvarez-Manzo ◽  
Ying Zhang

AbstractWe studied the effect of oxygen concentrations on the in vitro growth and drug susceptibility of Babesia duncani. We found that the growth of B. duncani required high level oxygen and the culture condition at ambient aerobic condition (21% O2) was optimal. Compared with ambient air, our results further showed that low oxygen (6-16%) could arrest B. duncani schizonts and lead to high susceptibilities to antiparasitic drugs atovaquone, pyrimethamine, quinine, and chloroquine at certain concentrations in vitro. Drug susceptibilities of other Babesia spp impacted by O2 levels need to be studied in the future, and this study indicates that culturing conditions of Babesia spp should be considered and reestablished for generating more comparable and reliable results in drug research in the future.Graphical abstractHighlightsThe in vitro growth of B. duncani requires high level oxygen and the culture condition of 5% CO2 ambient air is optimalLow level oxygen results B. duncani in low growth rate and high schizont ratio in vitroCompared with 5% CO2 ambient air, in vitro drug susceptibilities of B. duncani can be significantly increased when cultured in microaerobic condition


2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Xiao-Xing Wei ◽  
Wei-Tao Zheng ◽  
Xue Hou ◽  
Jian Liang ◽  
Zheng-Jun Li

The alcohol dehydrogenase promoterPadhEand mixed acid fermentation pathway deficient mutants ofEscherichia coliwere employed to produce poly(3-hydroxybutyrate) (P3HB) under microaerobic condition. TheE. colimutant withackA-pta, poxB, ldhA, andadhEdeletions accumulated 0.67 g/L P3HB, up to 78.84% of cell dry weight in tube cultivation. The deletion of pyruvate formate-lyase genepflBdrastically decreased P3HB production and P3HB content to 0.09 g/L and 24.44%, respectively. OverexpressingpflBvia the plasmid in its knocked out mutant restored cell growth and P3HB accumulation, indicating the importance of the pyruvate formate-lyase in microaerobic carbon metabolism. The engineeredE. coliBWapld (pWYC09) produced 5.00 g/L P3HB from 16.50 g/L glucose in 24 h batch fermentation, and P3HB production yield from glucose was 0.30 g/g, which reached up to 63% of maximal theoretical yield.


Sign in / Sign up

Export Citation Format

Share Document