Quantitative determination of nine water-soluble vitamins in the complex matrix of corn steep liquor for raw material quality assessment

2017 ◽  
Vol 92 (8) ◽  
pp. 2106-2113 ◽  
Author(s):  
Alexandra Hofer ◽  
Christoph Herwig
Keyword(s):  
Quantitative Determination ◽  
Quality Assessment ◽  
Corn Steep Liquor ◽  
Water Soluble ◽  
Raw Material ◽  
Complex Matrix ◽  
Material Quality ◽  
Steep Liquor

scholarly journals IMPROVING THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE RAW MATERIAL “ROSEHIP FRUIT”

2020 ◽  
pp. 131-138
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Vladimirovna Sharova ◽  
Polina Valeriyevna Afanaseva
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Water Soluble ◽  
Biologically Active ◽  
Raw Material ◽  
Test Quality ◽  
Total Carotenoids ◽  
Long Time

The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves. Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times. We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.

scholarly journals Quantitative determination of water-soluble polysaccharides in the aerial part of Potentilla anserina L.

2020 ◽  
Vol 20 (5-6) ◽  
pp. 191-196
Author(s):  
Ekaterina S. Tyutrina ◽  
Elena E. Saveleva ◽  
Vladimir I. Kurbatsky
Keyword(s):  
Quantitative Determination ◽  
Aerial Part ◽  
Federal District ◽  
Water Soluble ◽  
Raw Material ◽  
Siberian Federal District ◽  
Potentilla Anserina ◽  
Tomsk Region ◽  
Analysis Error

The article presents the gravimetric technique which was developed for the quantitative determination of water-soluble polysaccharides (WSPS) in the aerial part of Potentilla goose (Potentilla anserina). The purpose of the work was to develop the method of the quantitative determination of WSPS and the determination of their content in the aerial part of Potentilla anserina L., collected in various regions of Siberian federal district. Materials and methods. The object of the study was the aerial part of Potentilla anserina L, collected in June during flowering. To develop the method for the quantitative determination of the WSPS, herb mixtures collected in 2016 in vil. Verkhneussinskoe of Ermakovsky district, Krasnoyarsk Territory were studied. The amount of WSPS was determined in the above-ground part of Potentilla anserina L., collected during flowering in June 2017 in the Krasnoyarsk Territory (vil. Sliznevo), Tomsk Region (Tomsk Region), and the Republic of Buryatia (Zaigraevsky District, vil. Staraya Bryan). The above-ground parts were air dried in the shade. Determination of water-soluble polysaccharides was carried out gravimetrically in order to determine the optimal conditions for the maximum yield of water-soluble polysaccharides There was some variety in the degree of grinding of raw material, raw material / extractant index, extraction ratio, the ratio of extraction and ethyl alcohol 95%. Results. The greatest amount of water-soluble polysaccharides was extracted in the following conditions: the degree of grinding of raw material of 0.5-1.0 mm; the ratio of raw material and extractant was 1 : 10; the extraction rate was 5; the ratio of extraction and ethyl alcohol 95% was 1 : 4. The content of water-soluble polysaccharides was 7.55 0.36%, the analysis error did not exceed 5%. Conclusion. A gravimetric technique for the quantitative determination of water-soluble polysaccharides in the aerial part of P. anserina L has been developed. The content of water-soluble polysaccharides in the aerial part of P. anserina L. collected in various regions of the Siberian Federal District ranges from 5.95% (Krasnoyarsk Region) to 10.25% (Republic of Buryatia), the analysis error does not exceed 5%.

The Development of a Turbidimetric Method for the Quantitative Determination of Antibiotics of the Aminoglycoside Group in Medications

2020 ◽  
Vol 65 (7-8) ◽  
pp. 37-41
Author(s):  
E. N. Semenova ◽  
S. I. Kuleshova ◽  
E. I. Sakanyan
Keyword(s):  
Quantitative Determination ◽  
Quality Assessment ◽  
Aminoglycoside Antibiotics ◽  
Metrological Characteristics ◽  
Streptomycin Sulfate ◽  
Turbidimetric Method ◽  
Validation Criteria ◽  
Validation Parameters ◽  
Validation Tests

A method for the quantitative determination of streptomycin sulfate in medicines by the turbidimetric method has been developedand validated. Based on the results of the experiments, it was found that the metrological characteristics of such validation parameters of the method as linearity, precision, and correctness do not exceed the validation criteria. Linearity was noted in the range of streptomycin concentrations from 3.75 to 8.43 μg/ml. The results of validation tests of the method for the quantitative determination of streptomycin indicate the prospects and feasibility of introducing the turbidimetric method into the domestic system for standardization and quality assessment of aminoglycoside antibiotics.

scholarly journals Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Genan Wang ◽  
Bingyi Shi ◽  
Pan Zhang ◽  
Tingbin Zhao ◽  
Haisong Yin ◽  
...  
Keyword(s):  
Malic Acid ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Enrichment Analysis ◽  
Tca Cycle ◽  
Water Soluble ◽  
Growth And Yield ◽  
The Tca Cycle ◽  
Steep Liquor ◽  
Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

Quantitative determination of water-soluble scavengers in neoplastic and non-neoplastic human breast tissue

1988 ◽  
Vol 41 (5) ◽  
pp. 690-694 ◽  
Author(s):  
C. G. Honegger ◽  
J. Torhorst ◽  
H. Langemann ◽  
A. Kabiersch ◽  
W. Krenger
Keyword(s):  
Quantitative Determination ◽  
Breast Tissue ◽  
Water Soluble ◽  
Human Breast ◽  
Human Breast Tissue

scholarly journals Synthesis of the Microbial Polysaccharide Gellan from Dairy and Plant-Based Processing Coproducts

2021 ◽  
Vol 2 (2) ◽  
pp. 234-244
Author(s):  
Thomas P. West
Keyword(s):  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Culture Conditions ◽  
Water Soluble ◽  
Production Costs ◽  
Microbial Polysaccharide ◽  
Steep Liquor ◽  
The Cost ◽  
Condensed Distillers Solubles

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.

DEVELOPMENT OF A METHOD FOR QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN TRIPLEUROSPERMUM INODORUM’S FLOWERS

2021 ◽  
pp. 157-166
Author(s):  
Blinova O.L. ◽  
Gileva A.A. ◽  
Hlebnikov A.V. ◽  
Belonogova V.D. ◽  
Turyshev A.Y.
Keyword(s):  
Quantitative Determination ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Uv Spectra ◽  
Raw Material ◽  
Spectral Studies ◽  
Scientific Medicine ◽  
Differential Spectrophotometry ◽  
The Stability

Chamomilla recutita is used in scientific medicine. Tripleurospermum inodorum (L.) Sch. Bip. is widely spread among possible impurities of Chamomilla recutita (L.) Rauschert. This plant is perspective for establishment into scientific medicine but it can’t change Chamomilla recutita (L.) Rauschert. Purpose of research: development of a method for the quantitative determination of the amount of flavonoids in terms of rutin in Tripleurospermum inodorum’s flowers using differential spectrophotometry. Materials and methods. The samples prepared in different parts of Russia were used as objects of research. (2017 – 2020). Spectral studies were carried out in the wavelength range of 350-430 nm with a step of 1 nm using a spectrophotometer SF-2000. Results. To determine the analytical wavelength, the UV spectra of alcohol extraction of Tripleurospermum inodorum’s flowers were studied. Maximum of absorption was noticed at wavelength 370 nm. The differential spectrum of the same extraction with an aluminum chloride solution of 2% in 96% alcohol has a maximum at a length of 410±2 nm, which coincides with the maximum of the Standard Sample (SS) of rutin. The largest number of flavonoids is extracted by 70% alcohol. The maximum optical density and the highest output of the number of flavonoids from the raw material is observed at a degree of grinding of 2 mm with a single extraction for 60 minutes. In the conditions of complexation, the optimal ratio of the volume of the test solution and aluminum chloride with a solution of 2% in 96% alcohol is the ratio of 1:1. The stability of the complex with an aluminum chloride solution of 2% in 96% alcohol is observed in 40 minutes after the start of the reaction and retains it for 30 minutes. Findings. The method is developed and the parameters of the quantitative determination of the amount of flavonoids in Tripleurospermum inodorum flores are determined in terms of rutin using differential spectrophotometry.

MICROBIAL PENTOSANASES: II. SOME FACTORS AFFECTING THE PRODUCTION OF PENTOSANASES BY BACILLUS PUMILUS AND BACILLUS SUBTILIS

1956 ◽  
Vol 2 (1) ◽  
pp. 28-38 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Yeast Extract ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Corn Steep Liquor ◽  
Ammonium Phosphate ◽  
Water Soluble ◽  
High Rate ◽  
Mineral Salts ◽  
Ammonium Lactate ◽  
Steep Liquor

A number of carbohydrates and nitrogenous adjuncts were tested for their effect on the constitutive and adaptive pentosanases produced by Bacillus stibtilis and B. pumilus respectively in a medium containing biotin, ammonium phosphate, and other mineral salts. B. subtilis produced more enzyme with sulphite liquor than with any of the other carbohydrate sources tested. Next, in decreasing order of merit, were wheat bran, maltose, ribose, beet molasses, oat hulls, and pectin. Of the nitrogenous adjuncts tested, corn steep liquor, soybean meal, gelatin, gelysate, and ammonium lactate doubled the yield of enzyme whereas yeast extract, peptone, urea, and others were less effective. For B. pumilus the better carbohydrate sources, in decreasing order of merit, were wheat bran, water soluble pentosan of wheat flour, xylan, straw holo-cellulose, wheat straw, and sulphite liquor. Of the nitrogen sources, corn steep liquor was outstanding while casein, casitone, phytone, yeast extract, distillers' dried solubles, and soybean meal followed in decreasing order. A medium containing 6% wheat bran (20 mesh), 1% corn steep liquor neutralized with ammonia, 0.05% sodium chloride, and 0.05% calcium carbonate was devised for the production of pentosanase by B. pumilus. With this medium in shaken Erlenmeyer flasks, the enzyme was produced at a high rate between 12 and 40 hr.; thereafter the rate of production decreased. Maximum yields were obtained in 96 hr. A temperature of 26 °C. was more favorable for pentosanase production than higher temperatures.

Quantitative Determination of Cadmium in Water-Soluble Color Additives by Atomic Absorption Spectroscopy

1972 ◽  
Vol 55 (5) ◽  
pp. 1145-1149
Author(s):  
Lueangier Moten
Keyword(s):  
Aqueous Solutions ◽  
Quantitative Determination ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Quantitative Method ◽  
Water Soluble ◽  
Low Levels ◽  
Absorption Measurements

Abstract A quantitative method is presented for the determination of cadmium at low levels (5–20 ppm) in water-soluble color additives by atomic absorption spectroscopy. Absorption measurements were made on aqueous solutions of typical color additives to which known amounts of cadmium had been added. The method requires no pretreatment of sample and should be applicable to all water-soluble color additives.

Determination of vitamins B2, B3, B6 and B7 in corn steep liquor by NIR and PLSR

2012 ◽  
Vol 18 (5) ◽  
pp. 372-377 ◽  
Author(s):  
Xue Xiao ◽  
Yuanyuan Hou ◽  
Jun Du ◽  
Dan Sun ◽  
Gang Bai ◽  
...  
Keyword(s):  
Corn Steep Liquor ◽  
Steep Liquor
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