Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system

2016 ◽  
Vol 92 (3) ◽  
pp. 492-503 ◽  
Author(s):  
Sara M Badenes ◽  
Tiago G Fernandes ◽  
Cláudia C Miranda ◽  
Annette Pusch-Klein ◽  
Simone Haupt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dynamic System ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Expansion and long-term maintenance of induced pluripotent stem cells in stirred suspension bioreactors

2011 ◽  
Vol 6 (6) ◽  
pp. 462-472 ◽  
Author(s):  
Mehdi Shafa ◽  
Kirsten Sjonnesen ◽  
Akihiro Yamashita ◽  
Shiying Liu ◽  
Marek Michalak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Term Maintenance

scholarly journals Long-Term Retinal Differentiation of Human Induced Pluripotent Stem Cells in a Continuously Perfused Microfluidic Culture Device

2018 ◽  
Vol 14 (3) ◽  
pp. 1800323 ◽  
Author(s):  
Nima Abdolvand ◽  
Rui Tostoes ◽  
William Raimes ◽  
Vijay Kumar ◽  
Nicolas Szita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Retinal Differentiation ◽  
Induced Pluripotent

scholarly journals CtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cells

2017 ◽  
Vol 8 (2) ◽  
pp. 432-445 ◽  
Author(s):  
Daniel Gómez-Cabello ◽  
Cintia Checa-Rodríguez ◽  
María Abad ◽  
Manuel Serrano ◽  
Pablo Huertas
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Reprogramming ◽  
Long Term Consequences ◽  
Induced Pluripotent

scholarly journals Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells

2012 ◽  
Vol 9 (4) ◽  
pp. 375-388 ◽  
Author(s):  
Jing Jing Yang ◽  
Jian Fang Liu ◽  
Takayuki Kurokawa ◽  
Kazuhiro Kitada ◽  
Jian Ping Gong
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Self Renewal ◽  
Induced Pluripotent

scholarly journals Potential Threats of Nanoplastic Accumulation in Human Induced Pluripotent Stem Cells

2021 ◽  
Author(s):  
Hyejoong Jeong ◽  
Wijin Kim ◽  
Daheui Choi ◽  
Jiwoong Heo ◽  
Uiyoung Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cellular Functions ◽  
Differentiation Potential ◽  
Developmental Toxicology ◽  
Neural Lineage ◽  
Induced Pluripotent ◽  
The Impact

Abstract Background: Micro- and nanoplastics (NPs) produced from the bulk fragmentation are rampant in the world by enormous plastic trash everyday life. NPs can be exposed to humans through a variety of routes, including inhalation and intake. The threat to humans from NPs is increasing invisibly. Nowadays, many people are concerned about human safety and health, but few are reported about the effects of NP on humans. To overcome the limitations in human studies, human induced pluripotent stem cells (hiPSCs) were used as an optimal in vitro platform to investigate developmental toxicology and subtle changes on cellular functions in terms of differentiation potential throughout a long-term culture. Results: Negatively charged polystyrene nanoplastics (PS NPs) were used to exclude acute toxic issues of surface charge and investigate the impact of the NP's size and nature during bioaccumulation. Intracellular observations revealed that NPs up to 1000 nm were over-internalized into single cells within 48 h, and smaller NPs demonstrated greater potency at decreasing number of viable cells by a strong correlation with the number of NPs on an equal mass basis. Also, PS NPs caused a significant reduction in self-renewal capacity of hiPSCs for 48 h. After the cells were exposed to PS NPs for 48 to 96 h at the beginning of the differentiation process, NPs accumulated in hiPSC did not render cellular functions vulnerable or adversely affect EB formation, EB-mediated differentiation, and neural lineage differentiation for up to 14 days.Conclusion: This study confirmed that hiPSC exposure to polystyrene nanoplastics results in acute toxicity and non-significant long-term effects on cellular functions. This report is important for understanding the developmental toxicology of nanoplastics and the origin of disease.

186 Improving Success Rate of Establishment and Maintenance of Porcine Induced Pluripotent Stem Cells by Investigation of Colony Morphology

2018 ◽  
Vol 30 (1) ◽  
pp. 233
Author(s):  
P. Setthawong ◽  
P. Phakdeedindan ◽  
M. Techakumphu ◽  
T. Tharasanit
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Induced Pluripotent Stem Cells ◽  
Success Rate ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Ips Cells ◽  
Fibroblast Cells ◽  
Induced Pluripotent

Induced pluripotent stem cells (iPS cells) are generated by reprogramming of somatic cells using ectopic introduction of 4 transcription factors, including OCT4, SOX2, KLF4, and c-MYC (OSKM). Fibroblast cells are the most commonly used as a primary cell source for iPS cells because they are easy to harvest and culture. However, reprogramming efficiency of porcine fibroblasts is poor (~0.1%). During reprogramming process, mixed populations of primary colonies become the major obstacle in iPS establishment. In this study, we characterised 2 different colony morphologies at colony pick-up (compact and loose iPS morphology). We hypothesised that compact colonies will proceed to long-term culture and pluripotency. The fibroblast cells were isolated from tails of piglets and transfected with retroviral vectors expressing OSKM. The primary colonies were counted on Day 7 after gene transduction. The iPS-like colonies were divided into compact (n = 10) and loose (n = 10) morphology at colony pick-up. The characteristics of iPS-like cell lines were analysed by morphology, alkaline phosphatase staining, G-banding karyotype, immunofluorescence staining (OCT4), pluripotent gene expression (RT-PCR), and embryoid body formation. A total of 1,697 iPS-like colonies (2.34%) were observed. The compact colonies contained with tightly packed cells with distinct border between iPS colony and feeder cells, while colonies with irregular shape and border were classified as loose colonies. These 2 types of iPS-like colonies expressed alkaline phosphatase and OCT4. A total 5 of 10 (50%) compact morphology cell lines could be maintained in the undifferentiated state for more than 50 passages. But only 3 of 10 (30%) loose morphology cell lines demonstrated pluripotent characteristics. G-Banding karyotype analysis revealed normal chromosome number (n = 38). All of the cell lines also expressed endogenous pluripotent genes, including OSKM and NANOG and formed three-dimensional aggregating masses. In this study, we found that the cell lines from compact morphology could be maintained for longer than those of the loose morphology. A high rate of differentiation of loose iPS colony may also indicate that this type of colony has different pluripotency signals or incomplete reprogramming compared with compact colony. In conclusion, selection of compact colony morphology at colony pick-up is simple and reflects long-term propagation of porcine iPS cell lines. This information is important for improving the success rate of establishment and maintenance of porcine iPS cells.

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