scholarly journals Long-Term Retinal Differentiation of Human Induced Pluripotent Stem Cells in a Continuously Perfused Microfluidic Culture Device

2018 ◽  
Vol 14 (3) ◽  
pp. 1800323 ◽  
Author(s):  
Nima Abdolvand ◽  
Rui Tostoes ◽  
William Raimes ◽  
Vijay Kumar ◽  
Nicolas Szita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Retinal Differentiation ◽  
Induced Pluripotent

scholarly journals Expansion and long-term maintenance of induced pluripotent stem cells in stirred suspension bioreactors

2011 ◽  
Vol 6 (6) ◽  
pp. 462-472 ◽  
Author(s):  
Mehdi Shafa ◽  
Kirsten Sjonnesen ◽  
Akihiro Yamashita ◽  
Shiying Liu ◽  
Marek Michalak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Term Maintenance

Long-term expansion of human induced pluripotent stem cells in a microcarrier-based dynamic system

2016 ◽  
Vol 92 (3) ◽  
pp. 492-503 ◽  
Author(s):  
Sara M Badenes ◽  
Tiago G Fernandes ◽  
Cláudia C Miranda ◽  
Annette Pusch-Klein ◽  
Simone Haupt ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dynamic System ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals CtIP-Specific Roles during Cell Reprogramming Have Long-Term Consequences in the Survival and Fitness of Induced Pluripotent Stem Cells

2017 ◽  
Vol 8 (2) ◽  
pp. 432-445 ◽  
Author(s):  
Daniel Gómez-Cabello ◽  
Cintia Checa-Rodríguez ◽  
María Abad ◽  
Manuel Serrano ◽  
Pablo Huertas
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Reprogramming ◽  
Long Term Consequences ◽  
Induced Pluripotent

scholarly journals Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Guilan Li ◽  
Bingbing Xie ◽  
Liwen He ◽  
Tiancheng Zhou ◽  
Guanjie Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Disease Modeling ◽  
Cell Types ◽  
Retinal Cell ◽  
Retinal Differentiation ◽  
Induced Pluripotent ◽  
Urine Cells

Urine cells, a body trash, have been successfully reprogrammed into human induced pluripotent stem cells (U-hiPSCs) which hold a huge promise in regenerative medicine. However, it is unknown whether or to what extent U-hiPSCs can generate retinal cells so far. With a modified retinal differentiation protocol without addition of retinoic acid (RA), our study revealed that U-hiPSCs were able to differentiate towards retinal fates and form 3D retinal organoids containing laminated neural retina with all retinal cell types located in proper layer as in vivo. More importantly, U-hiPSCs generated highly mature photoreceptors with all subtypes, even red/green cone-rich photoreceptors. Our data indicated that a supplement of RA to culture medium was not necessary for maturation and specification of U-hiPSC-derived photoreceptors at least in the niche of retinal organoids. The success of retinal differentiation with U-hiPSCs provides many opportunities in cell therapy, disease modeling, and drug screening, especially in personalized medicine of retinal diseases since urine cells can be noninvasively collected from patients and their relatives.

scholarly journals Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells

2012 ◽  
Vol 9 (4) ◽  
pp. 375-388 ◽  
Author(s):  
Jing Jing Yang ◽  
Jian Fang Liu ◽  
Takayuki Kurokawa ◽  
Kazuhiro Kitada ◽  
Jian Ping Gong
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Self Renewal ◽  
Induced Pluripotent
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