Comparison of ionic liquid, acid and alkali pretreatments for sugarcane bagasse enzymatic saccharification

2011 ◽  
Vol 86 (10) ◽  
pp. 1342-1348 ◽  
Author(s):  
Li Wan Yoon ◽  
Gek Cheng Ngoh ◽  
Adeline Seak May Chua ◽  
Mohd. Ali Hashim
2016 ◽  
Vol 86 ◽  
pp. 113-119 ◽  
Author(s):  
Ai Asakawa ◽  
Tomohiro Oka ◽  
Chizuru Sasaki ◽  
Chikako Asada ◽  
Yoshitoshi Nakamura

2013 ◽  
Vol 772 ◽  
pp. 246-251
Author(s):  
Wei Liao ◽  
Zhao Mei Wang ◽  
Bing Jie Li

onic liquid, 1-n-butyl-3-methylimidazolium chloride ([C4mi Cl) was used to pretreat sugarcane bagasse (SCB), followed by regeneration of SCB by addition of deionized water. The regenerated SCB exhibited enhancement in enzymatic saccharification with an average 3.1-fold enhancement in yield of reducing sugar produced compared to untreated SCB in the kinetic study. The maximum yield of glucose and reducing sugar produced were 2.6% and 4.3% respectively for untreated SCB, whereas the maximum yield of glucose and reducing sugar produced were more significant with value of 7.2% and 16.6% respectively for IL-pretreated SCB. This paper suggested that the IL-pretreatment of SCB could be a useful pretreatment method to increase the yield of sugars produced in enzymatic saccharification.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.


2013 ◽  
Vol 36 (9) ◽  
pp. 1559-1567 ◽  
Author(s):  
H. Yanfei ◽  
H. Xiaoxiang ◽  
C. Qing ◽  
Z. Lingxiao

2013 ◽  
Vol 48 (12) ◽  
pp. 1942-1946 ◽  
Author(s):  
Li-Qun Jiang ◽  
Zhen Fang ◽  
Xing-Kang Li ◽  
Jia Luo ◽  
Suet-Pin Fan

2017 ◽  
Vol 225 ◽  
pp. 191-198 ◽  
Author(s):  
Jose A. Pérez-Pimienta ◽  
Alejandra Vargas-Tah ◽  
Karla M. López-Ortega ◽  
Yessenia N. Medina-López ◽  
Jorge A. Mendoza-Pérez ◽  
...  

2013 ◽  
Vol 275-277 ◽  
pp. 1662-1665 ◽  
Author(s):  
Qiang Li ◽  
Juan Juan Fei ◽  
Xu Ding Gu ◽  
Geng Sheng Ji ◽  
Yang Liu ◽  
...  

This study aims to establish a natural cellulosic biomass pretreatment process using ionic liquid (IL) for efficient enzymatic hydrolysis and second generation bioethanol. The IL 1-Butyl-3-methylimidazolium Chloride/FeCl3 ([Bmim]Cl/FeCl3) was selected in view of its low temperature pretreatment ability and the potential of accelerating enzymatic hydrolysis, and it could be recyclable. The yield of reducing sugars from sugarcane residue pretreated with this IL at 80 oC for 1 h reached 46.8% after being enzymatically hydrolyzed for 24 h. Sugarcane residue regenerated were hydrolyzed more easily than that treated with water. The fermentability of the hydrolyzates, obtained after enzymatic saccharification of the regenerated sugarcane residue, was transformed into bioethanol using Candida shehatae. This microbe could absorb glucose and xylose efficiently, and the ethanol production was 0.38 g/g glucose within 30 h fermentation. In conclusion, the metal ionic liquid pretreatment in low temperature shows promise as pretreatment solvent for natural biomass.


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