Targeted mutagenesis of EOD3 gene in Brassica napus L. regulates seed production

2020 ◽  
Vol 236 (3) ◽  
pp. 1996-2007
Author(s):  
Muhammad H. U. Khan ◽  
Limin Hu ◽  
Miaoshan Zhu ◽  
Yungu Zhai ◽  
Shahid U. Khan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Production ◽  
Targeted Mutagenesis ◽  
Brassica Napus L

scholarly journals Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

2020 ◽  
Vol 18 (5) ◽  
pp. 1153-1168 ◽  
Author(s):  
Yungu Zhai ◽  
Kaidi Yu ◽  
Shengli Cai ◽  
Limin Hu ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Oil Production ◽  
Targeted Mutagenesis ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Yellow Seed Coat

Alfalfa leafcutting bee (Hymenoptera: Megachilidae) pollination of oilseed rape (Brassica napus L.) under isolation tents for hybrid seed production

2001 ◽  
Vol 81 (1) ◽  
pp. 199-204 ◽  
Author(s):  
J. J. Soroka ◽  
D. W. Goerzen ◽  
K. C. Falk ◽  
K. E. Bett
Keyword(s):  
Brassica Napus ◽  
Seed Production ◽  
Oilseed Rape ◽  
Hybrid Seed ◽  
Hybrid Seed Production ◽  
Stocking Rate ◽  
Megachile Rotundata ◽  
Male Sterile ◽  
Brassica Napus L ◽  
Stocking Rates

In this 3-yr field study, the activities of alfalfa leafcutting bees (Megachile rotundata Fabricius) (LCB) at varying stocking levels were monitored in shade cloth isolation tents containing male-sterile and male-fertile rows of oilseed rape Brassica napus L. to determine optimum conditions for hybrid seed production. Bumble bees, Bombus impatiens Cresson, were similarly tested in 1 yr; their pollination activities were minimal, and weight of seed produced on female lines in tents containing bumble bees was similar to that in tents without pollinators. Leafcutting bee activity, as measured by the number of female bees at the entrance to tunnels in the hive, at first increased, then decreased with increasing stocking rate. Duration of time spent visiting flowers did not vary with stocking rate, but was longer on male-fertile than on male-sterile flowers. Production of hybrid seed within tents varied with leafcutting bee stocking rate, with the highest seed yield achieved at stocking rates equivalent to three charges at weekly intervals of 400 000 leafcutting bees per hectare. At optimum LCB stocking rates, the method described provided sufficient seed quantity for small-plot multi-location field evaluation of oilseed rape hybrids. Key words: Megachile rotundata, alfalfa leafcutting bee, Bombus impatiens, Brassica napus, oilseed rape, hybrid seed production, isolation tents

SEED PRODUCTION OF HYBRID SUMMER RAPE IN THE FIELD USING THE POL CYTOPLASMIC MALE STERILITY SYSTEM: A FIRST ATTEMPT

1990 ◽  
Vol 70 (3) ◽  
pp. 611-618 ◽  
Author(s):  
R. PINNISCH ◽  
P. B. E. McVETTY
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Seed Production ◽  
Seed Yield ◽  
Hybrid Seed ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L ◽  
Total Seed ◽  
Seed Yields

Hybrid summer rape (Brassica napus L.) seed production blocks were established at two locations in Manitoba in 1986 and 1987 to examine the effect of distance from the pollen source on seed yield, (both total and hybrid) and percent hybridity of seed produced on rows of a male sterile line of the open pollinated population B. napus cultivar, Marnoo, possessing the pol cytoplasmic male sterility inducing cytoplasm. A 10:1 ratio of male sterile line (A-line) to pollen parent was employed. Leaf cutter bees (Megachile rotundata F.) were used as the pollen vector between the two parents. In 1986, no significant differences in total seed yield were found among A-line rows, while in 1987, significant differences in total seed yields of A-line rows were found. Differences in hybrid seed yields among A-line rows were significant for all locations and years. Leaf cutter bees were found to be effective pollinators of the A-line plants. Less than half and less than a third of the seed produced on the A-line rows in 1986 and 1987, respectively, was hybrid seed. The high percentage of non-hybrid seed present in the seed lot may have been due to incomplete male sterility of the Marnoo A-line population. Total seed yields, hybrid seed yields and percent hybridity all declined linearly as distance from the pollen source increased. Improvement in the degree of male sterility of the Marnoo A-line population and/or a reduction in the 10:1 ratio of parents, and subsequent maximum A-line row to R-line row distance, will be necessary if hybrid summer rape seed production using this pol CMS A-line is to be commercially viable.Key words: Brassica napus L., CMS, hybrid, hybridity

scholarly journals Knockout of two Bna MAX 1 homologs by CRISPR /Cas9‐targeted mutagenesis improves plant architecture and increases yield in rapeseed ( Brassica napus L.)

2019 ◽  
Vol 18 (3) ◽  
pp. 644-654 ◽  
Author(s):  
Ming Zheng ◽  
Liang Zhang ◽  
Min Tang ◽  
Jinglin Liu ◽  
Hongfang Liu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Architecture ◽  
Targeted Mutagenesis ◽  
Brassica Napus L
Sign in / Sign up

Export Citation Format

Share Document