scholarly journals Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

2020 ◽  
Vol 18 (5) ◽  
pp. 1153-1168 ◽  
Author(s):  
Yungu Zhai ◽  
Kaidi Yu ◽  
Shengli Cai ◽  
Limin Hu ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Oil Production ◽  
Targeted Mutagenesis ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Yellow Seed Coat

Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L

Euphytica ◽  
2006 ◽  
Vol 149 (3) ◽  
pp. 381-385 ◽  
Author(s):  
Zhiwen Liu ◽  
Tingdong Fu ◽  
Ying Wang ◽  
Jinxing Tu ◽  
Baoyuan Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Brassica Napus L ◽  
Yellow Seed ◽  
Caps Markers ◽  
Yellow Seed Coat

Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daryl J. Somers ◽  
Gerhard Rakow ◽  
Vinod K. Prabhu ◽  
Ken R.D. Friesen
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Rapd Markers ◽  
Major Gene ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Yellow Seed Coat

scholarly journals Embryonal Control of Yellow Seed Coat Locus ECY1 Is Related to Alanine and Phenylalanine Metabolism in the Seed Embryo of Brassica napus

2016 ◽  
Vol 6 (4) ◽  
pp. 1073-1081 ◽  
Author(s):  
Fulin Wang ◽  
Jiewang He ◽  
Jianghua Shi ◽  
Tao Zheng ◽  
Fei Xu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Yellow Seed ◽  
Seed Embryo ◽  
Phenylalanine Metabolism ◽  
Yellow Seed Coat

Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1077-1082 ◽  
Author(s):  
Daryl J Somers ◽  
Gerhard Rakow ◽  
Vinod K Prabhu ◽  
Ken RD Friesen
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Rapd Markers ◽  
Major Gene ◽  
Coat Colour ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Dh Population ◽  
Seed Trait ◽  
Yellow Seed Coat

The development of yellow-seeded Brassica napus for improving the canola-meal quality characteristics of lower fibre content and higher protein content has been restricted because no yellow-seeded forms of B. napus exist, and their conventional development requires interspecific introgression of yellow seed coat colour genes from related species. A doubled-haploid (DH) population derived from the F1 generation of the cross 'Apollo' (black-seeded) × YN90-1016 (yellow-seeded) B. napus was analysed via bulked segregant analysis to identify molecular markers associated with the yellow-seed trait in B. napus for future implementation in marker-assisted breeding. A single major gene (pigment 1) flanked by eight RAPD markers was identified co-segregating with the yellow seed coat colour trait in the population. This gene explained over 72% of the phenotypic variation in seed coat colour. Further analysis of the yellow-seeded portion of this DH population revealed two additional genes favouring 'Apollo' alleles, explaining 11 and 8.5%, respectively, of the yellow seed coat colour variation. The data suggested that there is a dominant, epistatic interaction between the pigment 1 locus and the two additional genes. The potential of the markers to be implemented in plant breeding for the yellow-seed trait in B. napus is discussed.Key words: Brassica napus, yellow seed, RAPD.

scholarly journals Activation of rape (Brassica napus L.) embryo during seed germination. V. The first zones of ultrastructural changes and their expansion

2014 ◽  
Vol 56 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Mieczysław Karaś
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Seed Coat ◽  
Basal Part ◽  
Ultrastructural Changes ◽  
Embryo Axis ◽  
Brassica Napus L ◽  
Root Cells ◽  
Basal Zone

In the germinating rape embryo the columella and basal part of hypocotyl undergo earliest activation. Its first ultrastructural symptom is the appearance of numerous ER vesicles after 3-6 h of seed swelling. Their number is the highest in the external layers of the columella and decreases in basipetal direction. Dermatogen cells in the basal zone of the hypocotyl contain the greatest amount of ER structures, whereas decreasing amounts are found in both directions along the embryo axis and centripetally. Further changes in the ER spread in a similar order. The vesicles merge and form a tubular and plate-like ER. Then, they disappear and are replaced by tubular and vesicular forms. The changes in the ER are gradually followed by ultrastructural symptoms of activation of mitochondria, plastids and dictyosomes. The highest number of ER structures and other organelles accumulate in root cells shortly before piercing of the seed coat. After germination their amount decreases and remains almost stable.

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

Electrospun Microbial-Encapsulated Composite-Based Plasticized Seed Coat for Rhizosphere Stabilization and Sustainable Production of Canola (Brassica napus L.)

2019 ◽  
Vol 67 (18) ◽  
pp. 5085-5095 ◽  
Author(s):  
Zahid Hussain ◽  
Muhammad Ali Khan ◽  
Farasat Iqbal ◽  
Muhammad Raffi ◽  
Fauzia Yusuf Hafeez
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Sustainable Production ◽  
Brassica Napus L

scholarly journals A Large Insertion in bHLH Transcription Factor BrTT8 Resulting in Yellow Seed Coat in Brassica rapa

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e44145 ◽  
Author(s):  
Xia Li ◽  
Li Chen ◽  
Meiyan Hong ◽  
Yan Zhang ◽  
Feng Zu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Rapa ◽  
Seed Coat ◽  
Bhlh Transcription Factor ◽  
Yellow Seed ◽  
Yellow Seed Coat ◽  
Large Insertion

scholarly journals Proanthocyanidin biosynthesis in the seed coat of yellow-seeded, canola quality Brassica napus YN01-429 is constrained at the committed step catalyzed by dihydroflavonol 4-reductaseThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

Botany ◽  
2009 ◽  
Vol 87 (6) ◽  
pp. 616-625 ◽  
Author(s):  
Leonid Akhov ◽  
Paula Ashe ◽  
Yifang Tan ◽  
Raju Datla ◽  
Gopalan Selvaraj
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
National Research Council ◽  
Plant Biotechnology ◽  
Phenylpropanoid Metabolism ◽  
Brassica Napus L ◽  
Dihydroflavonol Reductase ◽  
Regulatory Circuits ◽  
Seed Coats ◽  
Canola Quality

The yellow seed characteristic in Brassica napus  L. is desirable because of its association with higher oil content and better quality of oil-extracted meal. YN01-429 is a yellow-seeded canola-quality germplasm developed in Canada arising from several years of research. Seed-coat pigmentation is due to oxidized proanthocyanidins (PA; condensed tannins) derived from phenylpropanoids and malonyl CoA. We found PA accumulation to be most robust in young seed coats (20 d post anthesis; dpa) of a related black-seeded line N89-53 and only very little PA in YN01-429, which also contained much less extractable phenolics. The flavonol content, however, did not show as great a difference between these two lines. Furthermore, sinapine, a product of the general phenylpropanoid metabolism, was present at comparable levels in the embryos of both lines. Dihydroflavonol reductase (DFR) activity that commits phenolics to PA synthesis was lower in YN01-429 seed coats. The results of Southern blot and in silico analyses were indicative of two copies of the DFR gene in B. napus. Both copies were functional in YN01-429, ruling out homeoallelic repression or silencing, but together they showed very low expression levels (17-fold fewer transcripts) relative to DFR activity in N89-53 seed coats. These results collectively suggest that YN01-429 differs in regulatory circuits that impact the PA synthesis branch much more than the flavonol synthesis branch in the seed coats and such circuits do not impinge upon general phenylpropanoid metabolism in the embryos.

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