Biomechanical stimulation effects on the metabolism of adipocyte

2020 ◽  
Vol 235 (11) ◽  
pp. 8702-8713
Author(s):  
Lisa Mor‐Yossef Moldovan ◽  
Nadav Kislev ◽  
Maayan Lustig ◽  
Leslie Pomeraniec ◽  
Dafna Benayahu
Keyword(s):  
Biomechanical Stimulation

Mechanical Properties and Related Histological Alterations of Engineered Tendons In Vivo

2005 ◽  
Vol 288-289 ◽  
pp. 11-14 ◽  
Author(s):  
Ting Wu Qin ◽  
Shujiang Zhang ◽  
Zhi Ming Yang ◽  
Xiang Tao Mo ◽  
Xiu Qun Li
Keyword(s):  
Mechanical Properties ◽  
Collagen Synthesis ◽  
Biomechanical Properties ◽  
Biomechanical Test ◽  
Histological Alterations ◽  
Normal Tendon ◽  
Tendon Cells ◽  
Biomechanical Stimulation

The purpose of this research is to find out the interaction between histological alterations and mechanical properties of engineered tendon implanted in situ. Defects of 0.5cm-1.0cm were made at deep flexor tendons by surgical procedure. Engineered tendons using degradable scaffolds polyglytic acid (PGA) mesh and tendon cells were implanted to repair the defects. Chickens were killed respectively at 2 weeks, 4 weeks, 6 weeks, and 8 weeks after surgery. The implants were taken out for histological examination, biomechanical test, and collagen synthesis assay. The results showed that after surgery the PGA scaffolds degraded fast and took precedence of collagen synthesis. There were not enough amount and maturation of the collagen fibers of the new tendon at 2-8 weeks after surgery. The biomechanical properties of new tendons were less than those of the normal tendon. Therefore, it is necessary to construct engineered tendons with better degradation rate of scaffolds and suitable biomechanical stimulation so that more collagen synthesis and better biomechanical properties of new tendons can be developed early after implantation.

Evaluation of the input of biomechanical stimulation in the toxicity of deoxynivalenol in A-431 cells

2016 ◽  
Vol 258 ◽  
pp. S144
Author(s):  
G. Del Favero ◽  
L. Wölflingseder ◽  
S. Seriani ◽  
P. Gallina ◽  
O. Sbaizero ◽  
...  
Keyword(s):  
Biomechanical Stimulation

scholarly journals Deoxynivalenol induces structural alterations in epidermoid carcinoma cells A431 and impairs the response to biomechanical stimulation

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Giorgia Del Favero ◽  
Lydia Woelflingseder ◽  
Lukas Janker ◽  
Benjamin Neuditschko ◽  
Stefano Seriani ◽  
...  
Keyword(s):  
Carcinoma Cells ◽  
Epidermoid Carcinoma ◽  
Structural Alterations ◽  
Biomechanical Stimulation

Computational Simulation of a MEMS-Based Microactuator for Tissue Engineering Applications

2008 ◽  
Author(s):  
Joseph Keyes ◽  
Michael Junkin ◽  
Pak Kin Wong ◽  
Jonathan P. Vande Geest
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Mechanical Stimulation ◽  
Biological Function ◽  
Computational Simulation ◽  
3D Microstructure ◽  
Tissue Replacement ◽  
Engineered Tissue ◽  
Biomechanical Stimulation ◽  
The Relationship

The relationship between the 3D microstructure of tissue-engineered constructs (TECs) and their resulting mechanical and biological function is critical in providing TECs with clinically meaningful mechanical properties in reasonable incubation times. We hypothesize that the next generation of TECs must incorporate a controllable and optimized microstructure (and resulting mechanical properties) if they are to mechanically and biologically mimic tissue function. While the development of a robustly engineered tissue replacement will undoubtedly require simultaneous biochemical and biomechanical stimulation, this paper will focus on the development of a device to impose localized micro-mechanical stimulation.

scholarly journals Enhancing Algal Growth by Stimulation with LED Lighting and Ultrasound

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Shao-Yi Hsia ◽  
Shiuh-Kuang Yang
Keyword(s):  
Growth Rate ◽  
Algal Growth ◽  
Ultrasound Treatment ◽  
Health Food ◽  
Led Lighting ◽  
Natural Factors ◽  
Potential Market ◽  
Different Temperatures ◽  
Biomechanical Stimulation ◽  
Led Lights

Algae are not only rich in natural nutrients, but are also a high-priced health food. An important constituent called “growth factor” is extracted from algae and used as an ingredient in medical drugs, foods, cosmetics, and other products. Its enormous potential market should not be taken lightly. Algae are mostly found near coastal areas and their habitats are limited by a number of natural factors, leading to large labor and financial expenditures to harvest. This report describes our study of indoor algae production using LED lights and ultrasound and manipulating other growth factors at different temperatures. Ultrasound treatment at the alga’s natural resonant frequency was varied to determine optimal algal growth using the Taguchi method to plan and to analyze the experiments. The results were very satisfying, showing an 8.23% increase in the growth rate by the fifth day due to ultrasound treatment and an amazing 27.01% growth rate due to biomechanical stimulation.

The Interplay Between Biomechanical and Biochemical Factors Regulates Lumen Formation and Navigation of Endothelial Cell Sprouts

2010 ◽  
Author(s):  
Amir Shamloo ◽  
Sarah C. Heilshorn
Keyword(s):  
Growth Factor ◽  
Blood Vessels ◽  
3D Culture ◽  
Vascular Endothelial ◽  
Lumen Formation ◽  
Biomechanical Stimulation ◽  
Sprout Formation ◽  
Endothelial Growth Factor ◽  
2D To 3D ◽  
Factor Concentration

Angiogenesis is the process of forming new blood vessels that originate from pre-existing vessels. In early angiogenesis stages, endothelial cells (ECs) migrate from the lumen of developed blood vessels into the surrounding extracellular matrix (ECM). Through the coordinated actions of migration and proliferation, these ECs organize into tubular capillary-like structures called sprouts. In this study, 3D EC sprout formation was examined using a microfluidic device that enabled the separate and simultaneous tuning of biomechanical and biochemical stimuli (Fig. 1). While previous investigations have been performed on each of these factors individually1, 2, more recent studies have identified a critical interplay between the simultaneous effects of these two factors3. For example, we previously studied 2D EC chemotaxis in response to vascular endothelial growth factor (VEGF) gradients in the absence of biomechanical stimulation.4 In developing a model that enables precise specification of biochemical and biomechanical cues, we utilized a protocol that enables ECs to undergo a transition from the 2D to 3D culture environment mimicking angiogenic sprouting. Here we quantified the relative importance and combined consequences of discrete changes in matrix density, growth factor concentration, and growth factor gradient steepness during the stages of early sprout initiation, sprout elongation, sprout navigation, and lumen formation.

scholarly journals Elder Mice Exhibit More Severe Degeneration and Milder Regeneration in Temporomandibular Joints Subjected to Bilateral Anterior Crossbite

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuejiao Zhang ◽  
Xiaojie Xu ◽  
Peng Zhou ◽  
Qian Liu ◽  
Mian Zhang ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Caspase 3 ◽  
Degenerative Changes ◽  
Dental Occlusion ◽  
Micro Ct ◽  
Superficial Zone ◽  
Condylar Cartilage ◽  
Age Related ◽  
Temporomandibular Joints ◽  
Biomechanical Stimulation

Temporomandibular joints (TMJs) have a biomechanical relationship with dental occlusion. Aberrant occlusion initiates degenerative remodeling responses in TMJ condyles. Aging is a promoting factor of osteoarthritis (OA) development. The aim of this study was to assess the effect of aging on degenerative remodeling in TMJ condyles in response to occlusal biomechanical stimulation caused by the installation of aberrant prostheses and observe rehabilitation after their removal. The experiments involved 84 female C57BL/6J mice (42 at 6 weeks old and 42 at 28 weeks old). A bilateral anterior crossbite (BAC) model was developed, and the TMJs were sampled at 3, 7, and 11 weeks. BAC was removed at 7 weeks in a subset of mice, which accepted BAC treatment at 6 week of age, and maintained for another 4 weeks after BAC removal. TMJ changes were assessed with micro-CT, histomorphology, immunohistochemistry (IHC), and immunofluorescence staining assays. The results showed that BAC induced typical OA-like TMJ lesions that were more severe in the elder groups as evaluated by the acellular zones, clustered chondrocytes, fissures between cartilage and subchondral bone, reductions in matrix amount and the cartilage thickness as revealed by histomorphological measurements, and subchondral bone loss as detected on micro-CT images. IHC indicated significant increases in cleaved caspase-3-expressing cells and decreases in ki67-positive cells in the BAC groups. There were obvious age-dependent changes in the numbers of superficial zone cells and CD90-expressing cells. Supportively, cleaved caspase-3-expressing cells obviously increased, while ki67-expressing cells significantly decreased with aging. In the elder BAC groups, the superficial zone cells such as CD90-expressing cells were greatly reduced. At 11 weeks, the superficial zone cells were almost non-existent, and there were clear serrated injuries on the cartilage surface. BAC removal attenuated the degenerative changes in the condylar cartilage and subchondral bone. Notably, the rescue effect was more pronounced in the younger animals. Our findings demonstrate the impacts of aging on both TMJ degenerative changes in response to BAC and regenerative changes following BAC removal. The reduced number of chondro-progenitor cells in aged TMJ cartilage provides an explanation for this age-related decline in TMJ rehabilitative behaviors.

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