Pulsed electromagnetic fields promote bone formation by activating the sAC–cAMP–PKA–CREB signaling pathway

2018 ◽  
Vol 234 (3) ◽  
pp. 2807-2821 ◽  
Author(s):  
Yuan‐Yuan Wang ◽  
Xiu‐Ying Pu ◽  
Wen‐Gui Shi ◽  
Qing‐Qing Fang ◽  
Xin‐Ru Chen ◽  
...  
Keyword(s):  
Bone Formation ◽  
Signaling Pathway ◽  
Electromagnetic Fields ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

Amelioration of bone fragility by pulsed electromagnetic fields in type 2 diabetic KK-Ay mice involving Wnt/β-catenin signaling

2021 ◽  
Author(s):  
Xi Shao ◽  
Yongqing Yang ◽  
Zhifen Tan ◽  
Yuanjun Ding ◽  
Erping Luo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Formation ◽  
Electromagnetic Fields ◽  
Bone Microarchitecture ◽  
Bone Microstructure ◽  
Bone Fragility ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic ◽  
Ay Mice

Type 2 diabetes mellitus (T2DM) results in compromised bone microstructure and quality, and subsequently increased risks of fractures. However, it still lacks safe and effective approaches resisting T2DM bone fragility. Pulsed electromagnetic fields (PEMF) exposure has proven to be effective in accelerating fracture healing and attenuating osteopenia/osteoporosis induced by estrogen deficiency. Nevertheless, whether and how PEMF resist T2DM-associated bone deterioration remain not fully identified. The KK-Ay mouse was used as the T2DM model. We found that PEMF stimulation with 2 h/day for 8 weeks remarkably improved trabecular bone microarchitecture, decreased cortical bone porosity, and promoted trabecular and cortical bone material properties in KK-Ay mice. PEMF stimulated bone formation in KK-Ay mice, as evidenced by increased serum levels of bone formation (osteocalcin and P1NP), enhanced bone formation rate and increased osteoblast number. PEMF significantly suppressed osteocytic apoptosis and sclerostin expression in KK-Ay mice. PEMF exerted beneficial effects on osteoblast- and osteocyte-related gene expression in the skeleton of KK-Ay mice. Nevertheless, PEMF exerted no effect on serum biomarkers of bone resorption (TRAcP5b and CTX-1), osteoclast number or osteoclast-specific gene expression (TRAP and cathepsin K). PEMF upregulated gene expression of canonical Wnt ligands (including Wnt1, Wnt3a and Wnt10b), but not non-canonical Wnt5a. PEMF also upregulated skeletal protein expression of downstream p-GSK-3β and β-catenin in KK-Ay mice. Moreover, PEMF-induced improvement in bone microstructure, mechanical strength and bone formation in KK-Ay mice was abolished after intragastric administration with the Wnt antagonist ETC-159. Together, our results suggest that PEMF can improve bone microarchitecture and quality by enhancing the biological activities of osteoblasts and osteocytes, which are associated with the activation of the Wnt/β-catenin signaling pathway. PEMF might become an effective countermeasure against T2DM-induced bone deterioration.

Pulsed electromagnetic fields promote bone formation around dental implants inserted into the femur of rabbits

2000 ◽  
Vol 11 (4) ◽  
pp. 354-360 ◽  
Author(s):  
Hiroyuki Matsumoto ◽  
Morio Ochi ◽  
Yoshihiro Abiko ◽  
Yukito Hirose ◽  
Tohru Kaku ◽  
...  
Keyword(s):  
Bone Formation ◽  
Dental Implants ◽  
Electromagnetic Fields ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

Exposure of murine cells to pulsed electromagnetic fields rapidly activates the mTOR signaling pathway

2006 ◽  
Vol 27 (7) ◽  
pp. 535-544 ◽  
Author(s):  
Thomas E. Patterson ◽  
Yoshitada Sakai ◽  
Mark D. Grabiner ◽  
Michael Ibiwoye ◽  
Ronald J. Midura ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Electromagnetic Fields ◽  
Mtor Signaling ◽  
Pulsed Electromagnetic Fields ◽  
Mtor Signaling Pathway ◽  
Pulsed Electromagnetic
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