In vitro differentiation of unrestricted somatic stem cells into functional hepatic-like cells displaying a hepatocyte-like glucose metabolism

2010 ◽  
Vol 225 (2) ◽  
pp. 545-554 ◽  
Author(s):  
Simon Waclawczyk ◽  
Anja Buchheiser ◽  
Ulrich Flögel ◽  
Teja F. Radke ◽  
Gesine Kögler
Keyword(s):  
Stem Cells ◽  
Glucose Metabolism ◽  
In Vitro Differentiation ◽  
Somatic Stem Cells ◽  
Unrestricted Somatic Stem Cells

In vitro Differentiation of Human Cord Blood-Derived Unrestricted Somatic Stem Cells into Hepatocyte-Like Cells on Poly(ε-Caprolactone) Nanofiber Scaffolds

2009 ◽  
Vol 190 (3) ◽  
pp. 135-149 ◽  
Author(s):  
Seyed Mahmoud Hashemi ◽  
Masoud Soleimani ◽  
Seyed Shahrooz Zargarian ◽  
Vahid Haddadi-Asl ◽  
Naser Ahmadbeigi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
In Vitro Differentiation ◽  
Somatic Stem Cells ◽  
Human Cord Blood ◽  
Nanofiber Scaffolds ◽  
Unrestricted Somatic Stem Cells

In vitro differentiation of human cord blood-derived unrestricted somatic stem cells towards an endodermal pathway

Cytotherapy ◽  
2007 ◽  
Vol 9 (4) ◽  
pp. 362-378 ◽  
Author(s):  
S. Sensken ◽  
S. Waclawczyk ◽  
A.S. Knaupp ◽  
T. Trapp ◽  
J. Enczmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
In Vitro Differentiation ◽  
Somatic Stem Cells ◽  
Human Cord Blood ◽  
Unrestricted Somatic Stem Cells

Unrestricted somatic stem cells: interaction with CD34+ cells in vitro and in vivo, expression of homing genes and exclusion of tumorigenic potential

Cytotherapy ◽  
2011 ◽  
Vol 13 (3) ◽  
pp. 357-365 ◽  
Author(s):  
Kathrin Sonja Jeltsch ◽  
Teja Falk Radke ◽  
Stephanie Laufs ◽  
Frank Anton Giordano ◽  
Heike Allgayer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Somatic Stem Cells ◽  
Tumorigenic Potential ◽  
Cd34 Cells ◽  
In Vivo Expression ◽  
Unrestricted Somatic Stem Cells

In vitro Evaluation of Ferutinin on Proliferation and Osteogenesis differentiation in Human Unrestricted Somatic Stem Cells

Steroids ◽  
2021 ◽  
pp. 108862
Author(s):  
Zahra Mahmoudi ◽  
Abbas Saidi ◽  
Mehrdad Iranshahi ◽  
Neda Dadgar ◽  
Arezou Azizsoltani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Somatic Stem Cells ◽  
In Vitro Evaluation ◽  
Unrestricted Somatic Stem Cells

Epithelial In vitro Differentiation of Mesenchymal Stem Cells

2018 ◽  
Vol 13 (6) ◽  
pp. 409-422 ◽  
Author(s):  
Alvaro Sierra-Sanchez ◽  
Alexandra Ordonez-Luque ◽  
Olga Espinosa Ibanez ◽  
Antonio Ruiz-Garcia ◽  
Salvador Arias Santiago
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Differentiation

scholarly journals 22(R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Manisha Singh ◽  
Manish Jain ◽  
Samrat Bose ◽  
Ashutosh Halder ◽  
Tapas Chandra Nag ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Dental Pulp ◽  
Neuronal Cells ◽  
Human Mesenchymal Stem Cells ◽  
Wistar Rat ◽  
Human Mscs ◽  
In Vitro Differentiation

AbstractOxysterols play vital roles in the human body, ranging from cell cycle regulation and progression to dopaminergic neurogenesis. While naïve human mesenchymal stem cells (hMSCs) have been explored to have neurogenic effect, there is still a grey area to explore their regenerative potential after in vitro differentiation. Hence, in the current study, we have investigated the neurogenic effect of 22(R)-hydroxycholesterol (22-HC) on hMSCs obtained from bone marrow, adipose tissue and dental pulp. Morphological and morphometric analysis revealed physical differentiation of stem cells into neuronal cells. Detailed characterization of differentiated cells affirmed generation of neuronal cells in culture. The percentage of generation of non-DA cells in the culture confirmed selective neurogenic potential of 22-HC. We substantiated the efficacy of these cells in neuro-regeneration by transplanting them into Parkinson’s disease Wistar rat model. MSCs from dental pulp had maximal regenerative effect (with 80.20 ± 1.5% in vitro differentiation efficiency) upon transplantation, as shown by various behavioural examinations and immunohistochemical tests. Subsequential analysis revealed that 22-HC yields a higher percentage of functional DA neurons and has differential effect on various tissue-specific primary human MSCs. 22-HC may be used for treating Parkinson’s disease in future with stem cells.

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