In vitro differentiation of spermatogonial stem cells to functional sperm in zebrafish

2014 ◽  
Author(s):  
Noriyoshi Sakai
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation

scholarly journals Retinol Improves In Vitro Differentiation of Pre-Pubertal Mouse Spermatogonial Stem Cells into Sperm during the First Wave of Spermatogenesis

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0116660 ◽  
Author(s):  
Brahim Arkoun ◽  
Ludovic Dumont ◽  
Jean-Pierre Milazzo ◽  
Agathe Way ◽  
Amandine Bironneau ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation

IN VITRO DIFFERENTIATION OF HUMAN SPERMATOGONIAL STEM CELLS IN A THREE DIMENSIONAL (3D) TESTICULAR ORGANOID SYSTEM

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Kara E. McAbee ◽  
Nima Pourhabibi Zarandi ◽  
Anthony Atala ◽  
Hooman Sadri-Ardekani ◽  
Colin Bishop
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation

In vitro differentiation of chicken spermatogonial stem cells into adipocytes

2008 ◽  
Vol 5 (3) ◽  
pp. 263-268
Author(s):  
Yu Fei ◽  
Ge Jian-Hui ◽  
Ni Li-Gang ◽  
He Xian-Hong ◽  
Xu Qi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation ◽  
Differentiated Cells ◽  
Ppar Γ ◽  
Combined Use ◽  
Oil Red O Staining ◽  
Oil Red O ◽  
Better Than

AbstractSpermatogonial stem cells (SSCs), which were isolated from chicken (Gallus gallus) embryo testes 16 days after laying, were cultured, subcultured, and induced into adipocytes in vitro. The differentiated cells were identified by oil red-O staining. Dexamethasone, insulin and 3-isobutyl-1-methylxanthine (IBMX) were tested for their induction potential. About 7–21 days after induction, SSCs differentiated into adipocytes, and the resulting adipocytes strongly expressed peroxisome proliferation activation receptor-γ (PPAR-γ). The assay outcome showed that an optimal treatment consisted of dexamethasone, insulin and IBMX application for 3 days and insulin for 1 day (3 cycles), then insulin for 21 days. The differentiation ratio was 85%, better than the combined use of dexamethasone, insulin and IBMX (P<0.01). However, the combination of the three derivatives triggered a stronger induction than any of them used alone (P<0.01). This study has demonstrated the potential of chicken embryonic SSCs to differentiate in vitro into adipocytes.

scholarly journals Isolation and Culture of Pig Spermatogonial Stem Cells and Their in Vitro Differentiation into Neuron-Like Cells and Adipocytes

2015 ◽  
Vol 16 (11) ◽  
pp. 26333-26346 ◽  
Author(s):  
Xiaoyan Wang ◽  
Tingfeng Chen ◽  
Yani Zhang ◽  
Bichun Li ◽  
Qi Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation

scholarly journals In vitro differentiation of spermatogonial stem cells using testicular cells from Guangxi Bama mini-pig

2018 ◽  
Vol 19 (5) ◽  
pp. 592 ◽  
Author(s):  
Huimin Zhao ◽  
Junyu Nie ◽  
Xiangxing Zhu ◽  
Yangqing Lu ◽  
Xingwei Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
In Vitro Differentiation ◽  
Testicular Cells

Epithelial In vitro Differentiation of Mesenchymal Stem Cells

2018 ◽  
Vol 13 (6) ◽  
pp. 409-422 ◽  
Author(s):  
Alvaro Sierra-Sanchez ◽  
Alexandra Ordonez-Luque ◽  
Olga Espinosa Ibanez ◽  
Antonio Ruiz-Garcia ◽  
Salvador Arias Santiago
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Differentiation
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