Stepwise up-regulation of MicroRNA expression levels from replicating to reversible and irreversible growth arrest states in WI-38 human fibroblasts

2009 ◽  
Vol 221 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Olivier C. Maes ◽  
Harshini Sarojini ◽  
Eugenia Wang
Keyword(s):  
Human Fibroblasts ◽  
Growth Arrest ◽  
Microrna Expression ◽  
Expression Levels

scholarly journals Identification of cis- and trans-regulatory variation modulating microRNA expression levels in human fibroblasts

2010 ◽  
Vol 21 (1) ◽  
pp. 68-73 ◽  
Author(s):  
C. Borel ◽  
S. Deutsch ◽  
A. Letourneau ◽  
E. Migliavacca ◽  
S. B. Montgomery ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
Microrna Expression ◽  
Expression Levels ◽  
Regulatory Variation ◽  
Cis And Trans

Measuring MicroRNA Expression Levels in Oncology: from Samples to Data Analysis

2013 ◽  
Vol 18 (4) ◽  
pp. 273-287 ◽  
Author(s):  
Loris De Cecco ◽  
Matteo Dugo ◽  
Silvana Canevari ◽  
Maria Grazia Daidone ◽  
Maurizio Callari
Keyword(s):  
Data Analysis ◽  
Microrna Expression ◽  
Expression Levels

scholarly journals Long Non-coding RNA GAS5 Worsens Coronary Atherosclerosis Through MicroRNA-194-3p/TXNIP Axis

2021 ◽  
Author(s):  
Yanbing Li ◽  
Yu Geng ◽  
Boda Zhou ◽  
Xuejiao Wu ◽  
Ou Zhang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Coronary Atherosclerosis ◽  
Growth Arrest ◽  
Interacting Protein ◽  
Expression Levels ◽  
Thioredoxin Interacting Protein ◽  
Non Coding Rna ◽  
Proliferation And Apoptosis ◽  
Regulatory Effects ◽  
Long Non Coding Rna

AbstractIt is formerly conducted that long non-coding RNA growth arrest-specific 5 (GAS5) is involved in the process of coronary atherosclerosis (AS). The regulatory effects of GAS5 on the microRNA (miR)-194-3p/thioredoxin-interacting protein (TXNIP) axis in AS have been insufficiently explored yet. Thereafter, this work is started from GAS5/miR-194-3p/TXNIP axis in AS. AS rats were modeled to obtain their coronary vascular tissues and endothelial cells (ECs), in which GAS5, miR-194-3p, and TXNIP expression were tested. ECs were identified by immunohistochemistry. The mechanism among GAS5, miR-194-3p, and TXNIP was determined. ECs were transfected with inhibited GAS5 or overexpressed miR-194-3p to decipher their functions in proliferation and apoptosis of ECs in AS. Raised GAS5 and TXNIP and degraded miR-194-3p expression levels exhibited in AS. GAS5 bound to miR-194-3p while miR-194-3p targeted TXNIP. Depleting GAS5 or restoring miR-194-3p enhanced proliferation and depressed apoptosis of ECs in AS. This work clearly manifests that inhibited GAS5 facilitates the growth of ECs through miR-194-3p-targeted TXNIP in AS, consolidating the basal reference to the curing for AS.

scholarly journals Prognostic value of microRNA expression levels in pancreatic adenocarcinoma: a review of the literature

Oncotarget ◽  
2017 ◽  
Vol 8 (42) ◽  
pp. 73345-73361 ◽  
Author(s):  
Patrick Wald ◽  
X. Shawn Liu ◽  
Cory Pettit ◽  
Mary Dillhoff ◽  
Andrei Manilchuk ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Prognostic Value ◽  
Microrna Expression ◽  
Review Of The Literature ◽  
Expression Levels

Comparison of microRNA expression levels between initial and recurrent glioblastoma specimens

2013 ◽  
Vol 112 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Aysegül Ilhan-Mutlu ◽  
Adelheid Wöhrer ◽  
Anna Sophie Berghoff ◽  
Georg Widhalm ◽  
Christine Marosi ◽  
...  
Keyword(s):  
Recurrent Glioblastoma ◽  
Microrna Expression ◽  
Expression Levels

scholarly journals Human Papillomavirus E6 Proteins Mediate Resistance to Interferon-Induced Growth Arrest through Inhibition of p53 Acetylation

2007 ◽  
Vol 81 (23) ◽  
pp. 12740-12747 ◽  
Author(s):  
Christy Hebner ◽  
Melanie Beglin ◽  
Laimonis A. Laimins
Keyword(s):  
Human Papillomavirus ◽  
Human Fibroblasts ◽  
Antiviral Agents ◽  
Growth Arrest ◽  
Physiological Role ◽  
Human Keratinocytes ◽  
Hpv E6 ◽  
E6 And E7 ◽  
E7 Proteins ◽  
Mutant Forms

ABSTRACT The high-risk human papillomavirus (HPV) E6 and E7 proteins act cooperatively to mediate multiple activities in viral pathogenesis. For instance, E7 acts to increase p53 levels while E6 accelerates its rate of turnover through the binding of the cellular ubiquitin ligase E6AP. Interferons are important antiviral agents that modulate both the initial and persistent phases of viral infection. The expression of HPV type 16 E7 was found to sensitize keratinocytes to the growth-inhibitory effects of interferon, while coexpression of E6 abrogates this inhibition. Treatment of E7-expressing cells with interferon ultimately resulted in cellular senescence through a process that is dependent upon acetylation of p53 by p300/CBP at lysine 382. Cells expressing mutant forms of E6 that are unable to bind p300/CBP or bind p53 failed to block acetylation of p53 at lysine 382 and were sensitive to growth arrest by interferon. In contrast, mutant forms of E6 that are unable to bind E6AP remain resistant to the effects of interferon, demonstrating that the absolute levels of p53 are not the major determinants of this activity. Finally, p53 acetylation at lysine 382 was found not to be an essential determinant of other types of senescence such as that induced by overexpression of Ras in human fibroblasts. This study identifies an important physiological role for E6 binding to p300/CBP in blocking growth arrest of human keratinocytes in the presence of interferon and so contributes to the persistence of HPV-infected cells.

scholarly journals Role of p14ARF in Replicative and Induced Senescence of Human Fibroblasts

2001 ◽  
Vol 21 (20) ◽  
pp. 6748-6757 ◽  
Author(s):  
Wenyi Wei ◽  
Ruth M. Hemmer ◽  
John M. Sedivy
Keyword(s):  
Replicative Senescence ◽  
Human Fibroblasts ◽  
Growth Arrest ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Major Pathway ◽  
Telomere Shortening ◽  
Normal Human ◽  
Cell Strains

ABSTRACT Following a proliferative phase of variable duration, most normal somatic cells enter a growth arrest state known as replicative senescence. In addition to telomere shortening, a variety of environmental insults and signaling imbalances can elicit phenotypes closely resembling senescence. We used p53−/− and p21−/− human fibroblast cell strains constructed by gene targeting to investigate the involvement of the Arf-Mdm2-p53-p21 pathway in natural as well as premature senescence states. We propose that in cell types that upregulate p21 during replicative exhaustion, such as normal human fibroblasts, p53, p21, and Rb act sequentially and constitute the major pathway for establishing growth arrest and that the telomere-initiated signal enters this pathway at the level of p53. Our results also revealed a number of significant differences between human and rodent fibroblasts in the regulation of senescence pathways.

Can balneotherapy modify microRNA expression levels in osteoarthritis? A comparative study in patients with knee osteoarthritis

2017 ◽  
Vol 61 (12) ◽  
pp. 2153-2158 ◽  
Author(s):  
C. Giannitti ◽  
A. De Palma ◽  
N. A. Pascarelli ◽  
S. Cheleschi ◽  
N. Giordano ◽  
...  
Keyword(s):  
Comparative Study ◽  
Knee Osteoarthritis ◽  
Microrna Expression ◽  
Expression Levels
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