Anti-inflammatory effect of heme oxygenase 1: Glycosylation and nitric oxide inhibition in macrophages

2004 ◽  
Vol 202 (2) ◽  
pp. 579-590 ◽  
Author(s):  
Hui-Yi Lin ◽  
Shing-Chuan Shen ◽  
Yen-Chou Chen
Keyword(s):  
Nitric Oxide ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Nitric Oxide Inhibition ◽  
Anti Inflammatory ◽  
Oxide Inhibition ◽  
Inflammatory Effect

scholarly journals Induction of Heme Oxygenase-1 by Sodium 9-Hydroxyltanshinone IIA Sulfonate Derivative Contributes to Inhibit LPS-Mediated Inflammatory Response in Macrophages

2015 ◽  
Vol 36 (4) ◽  
pp. 1316-1330 ◽  
Author(s):  
Xin-Hua Liu ◽  
Xi-Ling Wang ◽  
Hong Xin ◽  
Dan Wu ◽  
Xiao-Ming Xin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Response ◽  
Heme Oxygenase ◽  
Inos Expression ◽  
Heme Oxygenase 1 ◽  
Zinc Protoporphyrin ◽  
Nuclear Factor ◽  
Anti Inflammatory ◽  
Underlying Mechanisms ◽  
Inflammatory Effect

Background/Aim: Sodium 9-acetoxyltanshinone IIA sulfonate (ZY-1A4), a novel compound derived from sodium 9-hydroxyltanshinone IIA sulfonate, was synthesized with potential biological activities. This study aimed to explore the effects of ZY-1A4 on lipopolysaccharide (LPS)-triggered inflammatory response and the underlying mechanisms. Methods: Activation of RAW264.7 macrophages was induced by LPS. The effects of ZY-1A4 on inducible nitric oxide synthase (iNOS) expression, nitric oxide (NO) generation, nuclear factor-κB (NF-κB) activation, heme oxygenase-1 (HO-1) expression, and nuclear factor-erythroid 2 related factor 2 (Nrf2) pathway were evaluated to elucidate its underlying mechanisms on inflammatory responses. Results: ZY-1A4 concentration-dependently reduced iNOS expression and NO production, and inhibited c-Jun-N-terminal kinase 1/2 (JNK1/2) phosphorylation and NF-κB activation in LPS-stimulated macrophages. In addition, ZY-1A4 concentration- and time-dependently induced HO-1 expression associated with degradation of Kelch-like ECH-associated protein 1 (Keap1) and nuclear translocation of Nrf2, while the effect of ZY-1A4 was abolished by a phosphoinositide 3-kinase (PI3K) inhibitor LY294002. Intriguingly, pharmacological inactivation of HO-1 with zinc protoporphyrin IX reversed anti-inflammatory effect of ZY-1A4, but the anti-inflammatory effect of ZY-1A4 was largely mimicked by HO-1 by-products carbon monoxide and bilirubin. Furthermore, the inhibitory effect of ZY-1A4 on LPS-induced iNOS expression and NO release was abolished by HO-1 siRNA or LY294002. Conclusion: Our results demonstrated that ZY-1A4 suppressed LPS-induced iNOS expression and NO generation via modulation of NF-κB activation and HO-1 expression. This new finding might shed light to the prevention and therapy of cardiovascular diseases.

Heme oxygenase-1 mediates the anti-inflammatory effect of mushroom Phellinus linteus in LPS-stimulated RAW264.7 macrophages

2006 ◽  
Vol 106 (3) ◽  
pp. 364-371 ◽  
Author(s):  
Byung-Chul Kim ◽  
Joung-Woo Choi ◽  
Hye-Young Hong ◽  
Sin-Ae Lee ◽  
Suntaek Hong ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Phellinus Linteus ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model

2011 ◽  
Vol 411 (2) ◽  
pp. 354-359 ◽  
Author(s):  
Soon Won Kwon ◽  
Eun Jeong Sohn ◽  
Dae Won Kim ◽  
Hoon Jae Jeong ◽  
Mi Jin Kim ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Raw 264.7 Cells ◽  
Heme Oxygenase 1 ◽  
Raw 264.7 ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect
Sign in / Sign up

Export Citation Format

Share Document