scholarly journals Molecular genotyping of G6PD mutations for neonates in Ningbo area

2021 ◽  
Author(s):  
Jiewen Pan ◽  
Danyan Zhuang ◽  
Qi Yu ◽  
Xiaoli Pan ◽  
Youwei Bao ◽  
...  
Keyword(s):  
Molecular Genotyping

Molecular genotyping of the mouse scid allele

2002 ◽  
Vol 260 (1-2) ◽  
pp. 303-304 ◽  
Author(s):  
Amy L Sealey ◽  
Nicole K Hobbs ◽  
Edward E Schmidt
Keyword(s):  
Molecular Genotyping

scholarly journals Molecular Analysis of Nondisjunction in Mice Heterozygous for a Robertsonian Translocation

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1219-1224
Author(s):  
Lara A Underkoffler ◽  
Laura E Mitchell ◽  
A Russell Localio ◽  
Shannon M Marchegiani ◽  
Justin Morabito ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Robertsonian Translocation ◽  
Metacentric Chromosome ◽  
Paternal Age ◽  
Chromosome 7 ◽  
Parental Origin ◽  
Molecular Genotyping ◽  
Factors Influencing ◽  
Meiotic Nondisjunction ◽  
Acrocentric Chromosomes

Abstract A Robertsonian translocation results in a metacentric chromosome produced by the fusion of two acrocentric chromosomes. Rb heterozygous mice frequently generate aneuploid gametes and embryos, providing a good model for studying meiotic nondisjunction. We intercrossed mice heterozygous for a (7.18) Robertsonian translocation and performed molecular genotyping of 1812 embryos from 364 litters with known parental origin, strain, and age. Nondisjunction events were scored and factors influencing the frequency of nondisjunction involving chromosomes 7 and 18 were examined. We concluded the following: The frequency of nondisjunction among 1784 embryos (3568 meioses) was 15.9%.Nondisjunction events were distributed nonrandomly among progeny. This was inferred from the distribution of the frequency of trisomics and uniparental disomics (UPDs) among all litters.There was no evidence to show an effect of maternal or paternal age on the frequency of nondisjunction.Strain background did not play an appreciable role in nondisjunction frequency.The frequency of nondisjunction for chromosome 18 was significantly higher than that for chromosome 7 in males.The frequency of nondisjunction for chromosome 7 was significantly higher in females than in males. These results show that molecular genotyping provides a valuable tool for understanding factors influencing meiotic nondisjunction in mammals.

scholarly journals Recombination within the HLA-D region. Correlation of molecular genotyping with functional data.

1984 ◽  
Vol 160 (1) ◽  
pp. 222-238 ◽  
Author(s):  
M A Robinson ◽  
E O Long ◽  
A H Johnson ◽  
R J Hartzman ◽  
B Mach ◽  
...  
Keyword(s):  
Functional Data ◽  
Southern Blot Analysis ◽  
Hla Typing ◽  
Subtle Difference ◽  
Molecular Genotyping ◽  
Recombinant Haplotype ◽  
Allelic Differences ◽  
D Region ◽  
The Individual ◽  
Hla Haplotypes

Molecular genotyping of the HLA-D/DR region in a family correlated with serologic and cellular typing data. It was further possible to predict a subtle difference in SB region-related functions from such molecular studies. A family that included an individual who inherited an HLA haplotype with a paternal recombination between HLA-B and the HLA-D/DR region was identified by classic HLA typing techniques. Segregation of HLA-D/DR region genes in this family was studied by Southern blot analysis using cDNA probes for DR alpha, DR beta, DC alpha, DC beta, and SB beta. Restriction enzyme fragment polymorphisms observed for every gene tested were in concordance with assigned HLA haplotypes (including the individual known to have inherited a paternal recombinant haplotype) with one exception: two HLA identical siblings were observed to have different SB beta restriction fragment patterns. Further testing revealed that one individual inherited a maternal HLA haplotype recombinant between the HLA-D/DR region and SB beta. Although both maternal SB alleles typed as SB4, allelic differences could be detected cellularly by primed lymphocytes and by the differential expression of a class II cell surface antigen using monoclonal antibody. Therefore, predicted and nonpredicted recombinant haplotypes were detected in a family by molecular genotyping.

scholarly journals Risk factors and Molecular genotyping of Brucella melitensis strains recovered from humans and their owned cattle in Upper Egypt

One Health ◽  
2021 ◽  
pp. 100281
Author(s):  
Nour H. Abdel-Hamid ◽  
Hazem M. Ghobashy ◽  
Eman I. Beleta ◽  
Essam M. Elbauomy ◽  
Rania I. Ismail ◽  
...  
Keyword(s):  
Risk Factors ◽  
Brucella Melitensis ◽  
Upper Egypt ◽  
Molecular Genotyping

Molecular genotyping of “Rizor” and “Holly” rhizomania resistances in sugar beet

Euphytica ◽  
2015 ◽  
Vol 206 (2) ◽  
pp. 427-431 ◽  
Author(s):  
Piergiorgio Stevanato ◽  
Marco De Biaggi ◽  
Chiara Broccanello ◽  
Enrico Biancardi ◽  
Massimo Saccomani
Keyword(s):  
Sugar Beet ◽  
Molecular Genotyping

Molecular Genotyping of HIV-1 Strains from Newly Infected Men Who Have Sex with Men in Harbin, China

2016 ◽  
Vol 32 (6) ◽  
pp. 595-600 ◽  
Author(s):  
Qing-Hai Li ◽  
Fu-Xiang Wang ◽  
Chao Yue ◽  
Jia-Ye Wang ◽  
Gang Jin ◽  
...  
Keyword(s):  
Molecular Genotyping ◽  
Sex With Men ◽  
Hiv 1
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