scholarly journals A novel multiplex qPCR method for assessing the comparative lengths of telomeres

2021 ◽  
Author(s):  
Guozhu Sun ◽  
Hui Cao ◽  
Yang bai ◽  
Jueheng Wang ◽  
Yuxun Zhou ◽  
...  
Keyword(s):  
Multiplex Qpcr ◽  
Qpcr Method

scholarly journals Development of a New Monochrome Multiplex qPCR Method for Relative Telomere Length Measurement in Cancer

Neoplasia ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 425-431 ◽  
Author(s):  
Paige N. Dahlgren ◽  
Kanokwan Bishop ◽  
Shatovisha Dey ◽  
Brittney-Shea Herbert ◽  
Hiromi Tanaka
Keyword(s):  
Telomere Length ◽  
Length Measurement ◽  
Multiplex Qpcr ◽  
Relative Telomere Length ◽  
Qpcr Method

scholarly journals Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method

2019 ◽  
Vol 68 (12) ◽  
pp. 1732-1739 ◽  
Author(s):  
Caifeng Ma ◽  
Jikun Du ◽  
Weina He ◽  
Rui Chen ◽  
Yuxia Li ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Accurate Diagnosis ◽  
Urogenital Tract ◽  
Multiplex Qpcr ◽  
Qpcr Method

Optimizing, validating, and field testing a multiplex qPCR for the detection of amphibian pathogens

2018 ◽  
Vol 129 (1) ◽  
pp. 1-13 ◽  
Author(s):  
I Standish ◽  
E Leis ◽  
N Schmitz ◽  
J Credico ◽  
S Erickson ◽  
...  
Keyword(s):  
Field Testing ◽  
Multiplex Qpcr ◽  
Amphibian Pathogens

scholarly journals Evaluation of a fully automated high-throughput SARS-CoV-2 multiplex qPCR assay with built-in screening functionality for del-HV69/70- and N501Y variants such as B.1.1.7

2021 ◽  
pp. 104894
Author(s):  
Dominik Nörz ◽  
Moritz Grunwald ◽  
Flaminia Olearo ◽  
Nicole Fischer ◽  
Martin Aepfelbacher ◽  
...  
Keyword(s):  
High Throughput ◽  
Qpcr Assay ◽  
Multiplex Qpcr

Large-scale comparison of E. coli levels determined by culture and a qPCR method (EPA Draft Method C) in Michigan towards the implementation of rapid, multi-site beach testing

2021 ◽  
Vol 184 ◽  
pp. 106186
Author(s):  
Richard Haugland ◽  
Kevin Oshima ◽  
Mano Sivaganesan ◽  
Alfred Dufour ◽  
Manju Varma ◽  
...  
Keyword(s):  
Large Scale ◽  
E Coli ◽  
Qpcr Method

scholarly journals Expression of BARD1 β Isoform in Selected Pediatric Tumors

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 168
Author(s):  
Anna Jasiak ◽  
Natalia Krawczyńska ◽  
Mariola Iliszko ◽  
Katarzyna Czarnota ◽  
Kamil Buczkowski ◽  
...  
Keyword(s):  
Pediatric Cancer ◽  
Genome Stability ◽  
Cell Cycle Control ◽  
Reverse Transcriptase Gene ◽  
Neoplastic Tissue ◽  
Adult Type ◽  
Qpcr Method ◽  
New Treatments ◽  
First Time ◽  
Work Supports

Currently, many new possible biomarkers and mechanisms are being searched and tested to analyse pathobiology of pediatric tumours for the development of new treatments. One such candidate molecular factor is BARD1 (BRCA1 Associated RING Domain 1)—a tumour-suppressing gene involved in cell cycle control and genome stability, engaged in several types of adult-type tumours. The data on BARD1 significance in childhood cancer is limited. This study determines the expression level of BARD1 and its isoform beta (β) in three different histogenetic groups of pediatric cancer—neuroblastic tumours, and for the first time in chosen germ cell tumours (GCT), and rhabdomyosarcoma (RMS), using the qPCR method. We found higher expression of beta isoform in tumour compared to healthy tissue with no such changes concerning BARD1 full-length. Additionally, differences in expression of BARD1 β between histological types of neuroblastic tumours were observed, with higher levels in ganglioneuroblastoma and ganglioneuroma. Furthermore, a higher expression of BARD1 β characterized yolk sac tumours (GCT type) and RMS when comparing with non-neoplastic tissue. These tumours also showed a high expression of the TERT (Telomerase Reverse Transcriptase) gene. In two RMS cases we found deep decrease of BARD1 β in post-chemotherapy samples. This work supports the oncogenicity of the beta isoform in pediatric tumours, as well as demonstrates the differences in its expression depending on the histological type of neoplasm, and the level of maturation in neuroblastic tumours.

Interlaboratory Validation of a Real-Time PCR 24-Hour Rapid Method for Detection of Salmonella in Foods

2009 ◽  
Vol 72 (5) ◽  
pp. 945-951 ◽  
Author(s):  
CHORNG-MING CHENG ◽  
KHANH T. VAN ◽  
WEN LIN ◽  
RICHARD M. RUBY
Keyword(s):  
Real Time ◽  
Screening Method ◽  
Rapid Screening ◽  
Selective Enrichment ◽  
Manual Method ◽  
Qpcr Method ◽  
Peptone Water ◽  
Interlaboratory Validation ◽  
Chili Powder ◽  
The One

The efficacy of a 24-h Salmonella real-time, or quantitative, PCR (qPCR) detection method was assessed through a collaborative effort involving eight Federal and state laboratories. Eleven foods including mashed potatoes, soft cheese, chili powder, chocolate, eggs, sprouts, apple juice, fish, shrimp, ground beef, and ground chicken were tested. For each food, seven blind samples were distributed to each participant for testing. These included six samples equivalently inoculated with 1 to 5 CFU/25 g of various serotypes of Salmonella (Gaminara, Weltevreden, Heidelberg, Senftenberg, Enteritidis, Newport, Typhimurium, and Kentucky for each food) and 10 to 50 CFU/25 g of the competitor Enterobacter cloacae. The seventh sample was inoculated with 10 to 50 CFU/25 g of the competitor, E. cloacae, only. These samples were tested for Salmonella by using four methods in parallel: (i) 24-h qPCR method detecting Salmonella from modified buffered peptone water enrichment medium; (ii) 48-h qPCR method detecting Salmonella from a secondary selective enrichment broth; (iii) modified Bacteriological Analytical Manual method; and (iv) VIDAS, an immunoassay system. The results of the statistical analysis showed there was no significant (P ≥ 0.05) difference between either of the qPCR methods and the modified Bacteriological Analytical Manual method for 10 of 11 foods. For the one exception, sprouts, detection by qPCR required 48 h. Both qPCR methods showed a detection limit of 0.08 to 0.2 CFU/g. These results provide a solid basis for using this 24-h qPCR rapid screening method to detect Salmonella in foods.

Mycobacterium avium subsp. avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

2014 ◽  
Vol 77 (1) ◽  
pp. 141-144 ◽  
Author(s):  
PETR KRIZ ◽  
MARIJA KAEVSKA ◽  
IVA SLANA ◽  
IVA BARTEJSOVA ◽  
IVO PAVLIK
Keyword(s):  
Lymph Nodes ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mesenteric Lymph Nodes ◽  
Tissue Samples ◽  
The Czech Republic ◽  
Mesenteric Lymph ◽  
Qpcr Method ◽  
Pig Carcasses ◽  
Slaughtered Pigs

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)–like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (102 to 103 cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.

scholarly journals Dynamics of λ-cyhalothrin disappearance and expression of selected P450 genes in bees depending on the ambient temperature

2021 ◽  
Vol 19 (1) ◽  
pp. 1251-1258
Author(s):  
Bartosz Piechowicz ◽  
Marika Kobielska ◽  
Anna Koziorowska ◽  
Magdalena Podbielska ◽  
Ewa Szpyrka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ambient Temperature ◽  
Honey Bees ◽  
Half Life ◽  
Degradation Rate ◽  
Plant Protection ◽  
Plant Protection Product ◽  
Cytochrome P450 Genes ◽  
Qpcr Method ◽  
Different Temperatures

Abstract Temperature has a significant influence on the action of pyrethroids, and their effect increases with decreasing ambient temperature. Using gas chromatography, we assessed the degradation rate of λ-cyhalothrin, active ingredients (AI) of Karate Zeon 050 CS from pyrethroid group, in bees incubated for 48 h under different temperature conditions. With RT-qPCR method, we studied expression levels of selected cytochrome P450 genes after exposure to the plant protection product (PPP). The half-life of λ-cyhalothrin decreased from 43.32 to 17.33 h in the temperature range of 21–31°C. In animals incubated at 16°C, the AI half-life was even shorter and amounted to 10.19 h. The increase in temperature increased the expression of Cyp9Q1, Cyp9Q2, and Cyp9Q3 in the group of control bees. We showed a two-fold statistically significant increase in gene expression after treatment with PPP bees. The obtained results indicate that honey bees are characterized by susceptibility to pyrethroids that vary depending on the ambient temperature. This may be due to the different expressions of genes responsible for the detoxification of these PPPs at different temperatures.

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