scholarly journals Rapid and accurate diagnosis of Chlamydia trachomatis in the urogenital tract by a dual-gene multiplex qPCR method

2019 ◽  
Vol 68 (12) ◽  
pp. 1732-1739 ◽  
Author(s):  
Caifeng Ma ◽  
Jikun Du ◽  
Weina He ◽  
Rui Chen ◽  
Yuxia Li ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Accurate Diagnosis ◽  
Urogenital Tract ◽  
Multiplex Qpcr ◽  
Qpcr Method

THE ROLE OF UROGENITAL INFECTIONS IN THE PATHOGENESIS OF THE DEVELOPMENT OF MALE INFERTILITY

Vestnik ◽  
2021 ◽  
pp. 161-165
Author(s):  
Ж. Инкарбек ◽  
Ж. Турсынбеков ◽  
Е. Чакен ◽  
А.Х. Касымов ◽  
С.Б. Шалекенов
Keyword(s):  
Chlamydia Trachomatis ◽  
Male Infertility ◽  
Diagnostic Methods ◽  
Urogenital Tract ◽  
World Health ◽  
Urogenital Infection ◽  
Urogenital Infections ◽  
The World ◽  
Tract Infections

Вопрос бесплодия на сегодняшний день остается актуальным как в Казахстане, так и во всем мире. На основании данных Всемирной Организации Здравоохранения, ежегодно число бесплодных пар составляет 4-5% от всеобщей популяции. Причин бесплодия - множество. Но одним из острых и первоочередных причин данной патологии является мужское бесплодие, частота возникновения которого составляет 48,8% от общего количества случаев. [1] По данным мировых исследований, в процентном соотношении причины мужского бесплодия составляют: бесплодие неясного генеза - 31,2%, варикоцеле - 14,7%, эндокринные нарушения - 8,45%, инфекции урогенитального тракта - 8,3%, иммунологические факторы - 4,8%, опухоли яичек - 1,17%. [2] The issue of infertility today remains relevant both in Kazakhstan and around the world. The World Health Organization accounts for 4-5% of the general population annually. There are many reasons for infertility. One of the acute and primary causes of this pathology is male infertility, the incidence of which is 48.8% of the total number of cases. According to world studies, the percentage of the causes of male infertility are: infertility of unknown origin - 31.2%, varicocele - 14.7%, endocrine disorders - 8.45%, infections of the urogenital tract - 8.3%, immunological factors - 4 , 8%, testicular tumors - 1.17%. Goal. Establishing the role of individual infections and their association of the urogenital tract in the development of male infertility. Materials and methods. The study was carried out on the basis of the GKP on the REM "City polyclinic No. 26" in Almaty. A retrospective analysis of individual cards of 538 men from 21 to 45 years old who consulted urologists with complaints of urination, pain and discomfort in the anogenital region and above the bosom from January to December 2020 was carried out. The patients were divided into two groups: the first group - 258 men suffering from infertility, and who have other causes of infertility, the second - 280 men with normal fertility. Diagnostic methods were selected: bacterial culture of urine and scraping from the urethra, PCR of urogenital infections. Result: The total dissemination of the urethra in patients of the first group is 3 times higher than in men of the second group. However, we were interested not only in the general dissemination and types of pathogens, as according to the literature [1,2] the most aggressive chlamydia trachomatis and ureoplasm. In our frequency of occurrence of chlamydial infection in both groups was 153 (59.3%) and 23 (8%); mycoplasma - 148 (57.3%) and 45 (16%); ureaplasma - 137 (53.1%) and 63 (22.5%); Candida - 98 (37.9%) and 35 (12.5%); Trichomonas - 87 (33.7%) and 48 (17.1%); gardnerella - 94 (36.4%) and 56 (20%) people, respectively. Conclusions. Urogenital tract infections are the main reason people see a doctor. Infections are one of the factors in the development of infertility in men. For infection of the urogenital tract, PCR is the most sensitive and accurate compared to urine culture and urethral scrapings. The total dissemination of urogenital infection in the group of men with infertility is 46.3%, in comparison with the group of men with a normal norm in whom the percentage of STIs is 16%, indicates a direct effect of urogenital infection on the development of the male population. Along with the revealed, as the main factor of influence of the reproductive system, chlamydia trachomatis 59.3%, mycoplasma 57.3%, ureoplasm 53.1%, which moved to the second and third place in the development of infertility in the studied men. This factor and methods of treatment will be studied in studies.

scholarly journals Rapid screening for Chlamydia trachomatis infection by detecting α-mannosidase activity in urogenital tract specimens

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Ze-yu Wang ◽  
Guang-yu Fu ◽  
Shan-mei Wang ◽  
Dong-chun Qin ◽  
Zhong-quan Wang ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Urogenital Tract ◽  
Rapid Screening ◽  
Chlamydia Trachomatis Infection

scholarly journals Development of a New Monochrome Multiplex qPCR Method for Relative Telomere Length Measurement in Cancer

Neoplasia ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. 425-431 ◽  
Author(s):  
Paige N. Dahlgren ◽  
Kanokwan Bishop ◽  
Shatovisha Dey ◽  
Brittney-Shea Herbert ◽  
Hiromi Tanaka
Keyword(s):  
Telomere Length ◽  
Length Measurement ◽  
Multiplex Qpcr ◽  
Relative Telomere Length ◽  
Qpcr Method

scholarly journals Draft Genome Sequence of Chlamydia trachomatis Strain 54, Isolated from the Urogenital Tract of a Male in Japan

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Tomohiro Yamazaki ◽  
Junji Matsuo ◽  
Momoka Kikuchi ◽  
Kentaro Miyamoto ◽  
Kentaro Oka ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Proteins ◽  
Adaptive Evolution ◽  
Genome Sequence ◽  
Sexual Differentiation ◽  
Draft Genome ◽  
Genomic Analysis ◽  
Urogenital Tract ◽  
Draft Genome Sequence ◽  
Selective Pressures

We report the draft genome sequence of Chlamydia trachomatis strain 54, isolated from the urogenital tract of a male in Japan, with unique polymorphic membrane proteins. Detailed genomic analysis will aid our understanding of the selective pressures that lead to sexual differentiation in chlamydial adaptive evolution.

scholarly journals Impact of a Low-Oxygen Environment on the Efficacy of Antimicrobials against Intracellular Chlamydia trachomatis

2011 ◽  
Vol 55 (5) ◽  
pp. 2319-2324 ◽  
Author(s):  
Kensuke Shima ◽  
Márta Szaszák ◽  
Werner Solbach ◽  
Jens Gieffers ◽  
Jan Rupp
Keyword(s):  
Multidrug Resistance ◽  
Chlamydia Trachomatis ◽  
Inflammatory Diseases ◽  
Antimicrobial Activities ◽  
Careful Consideration ◽  
Oxygen Availability ◽  
Urogenital Tract ◽  
Low Oxygen ◽  
Tubal Infertility ◽  
Content Type

ABSTRACTEmergence of chronic inflammation in the urogenital tract induced byChlamydia trachomatisinfection in females is a long-standing concern. To avoid the severe sequelae ofC. trachomatisinfection, such as pelvic inflammatory diseases (PID), ectopic pregnancies, and tubal infertility, antibiotic strategies aim to eradicate the pathogen even in asymptomatic and uncomplicated infections. Although first-line antimicrobials have proven successful for the treatment ofC. trachomatisinfection, treatment failures have been observed in a notable number of cases. Due to the obligate intracellular growth ofC. trachomatis, reliable antimicrobial susceptibility assays have to consider environmental conditions and host cell-specific factors. Oxygen concentrations in the female urogenital tract are physiologically low and decrease further during an inflammatory process. We compared MIC testing and time-kill curves (TKC) for doxycycline, azithromycin, rifampin, and moxifloxacin under hypoxia (2% O2) and normoxia (20% O2). While low oxygen availability only moderately decreased the antichlamydial activity of azithromycin in conventional MIC testing (0.08 μg/ml versus 0.04 μg/ml;P< 0.05), TKC analyses revealed profound divergences for antibiotic efficacies between the two conditions. Thus,C. trachomatiswas significantly less rapidly killed by doxycycline and azithromycin under hypoxia, whereas the efficacies of moxifloxacin and rifampin remained unaffected using concentrations at therapeutic serum levels. Chemical inhibition of multidrug resistance protein 1 (MDR-1), but not multidrug resistance-associated protein 1 (MRP-1), restored doxycycline activity against intracellularC. trachomatisunder hypoxia. We suggest careful consideration of tissue-specific characteristics, including oxygen availability, when testing antimicrobial activities of antibiotics against intracellular bacteria.

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