scholarly journals Selection and identification of an ssDNA aptamer to NB4 cell

2021 ◽  
Vol 35 (4) ◽  
Author(s):  
Xian‐hui Zhang ◽  
Wei Wang ◽  
Xin Chen
Keyword(s):  
Ssdna Aptamer

Rapid and Accurate Quantification of Paramylon Produced from Euglena gracilis Using an ssDNA Aptamer

2019 ◽  
Vol 68 (1) ◽  
pp. 402-408 ◽  
Author(s):  
Jee Young Kim ◽  
Jeong-Joo Oh ◽  
Da Hee Kim ◽  
Jaewon Park ◽  
Hyun Soo Kim ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Ssdna Aptamer ◽  
Accurate Quantification

Manufacturing of a novel double-function ssDNA aptamer for sensitive diagnosis and efficient neutralization of SEA

2018 ◽  
Vol 548 ◽  
pp. 69-77 ◽  
Author(s):  
Hamid Sedighian ◽  
Raheleh Halabian ◽  
Jafar Amani ◽  
Mohammad Heiat ◽  
Ramezan Ali Taheri ◽  
...  
Keyword(s):  
Double Function ◽  
Ssdna Aptamer

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

An ssDNA aptamer specific for detection and purification of hexahistidine-tagged proteins

2020 ◽  
Vol 607 ◽  
pp. 113893
Author(s):  
Shijia Wu ◽  
Yanting Liu ◽  
Hongguang Sun ◽  
Meizuo Zhong ◽  
Bichun Dai ◽  
...  
Keyword(s):  
Ssdna Aptamer

A novel borax-specific ssDNA aptamer screened by high-throughput SELEX and its colorimetric assay with aggregation of AuNPs

2021 ◽  
Vol 101 ◽  
pp. 103947
Author(s):  
Le Jing ◽  
Mingwei Qin ◽  
Xiaomeng Zhang ◽  
Yuzhu Song ◽  
Jinyang Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
Colorimetric Assay ◽  
Ssdna Aptamer

Imidazole-free purification of His 3 -tagged recombinant proteins using ssDNA aptamer-based affinity chromatography

2015 ◽  
Vol 1418 ◽  
pp. 130-139 ◽  
Author(s):  
Filip Bartnicki ◽  
Ewa Kowalska ◽  
Katarzyna Pels ◽  
Wojciech Strzalka
Keyword(s):  
Affinity Chromatography ◽  
Recombinant Proteins ◽  
Ssdna Aptamer

Development of a ssDNA aptamer for detection of residual benzylpenicillin

2017 ◽  
Vol 531 ◽  
pp. 1-7 ◽  
Author(s):  
A-Young Lee ◽  
Na-Reum Ha ◽  
In-Pil Jung ◽  
Sang-Heon Kim ◽  
A-Ru Kim ◽  
...  
Keyword(s):  
Ssdna Aptamer
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