scholarly journals Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP‐based multiplex‐PCR assay

2019 ◽  
Vol 34 (4) ◽  
Author(s):  
Huanhuan Huang ◽  
Suqing Chen ◽  
Xiaoyan Zhang ◽  
Linliang Hong ◽  
Yongbin Zeng ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Multiplex Pcr ◽  
Clinical Characteristics ◽  
Respiratory Tract Infections ◽  
Respiratory Viruses ◽  
Hospitalized Children ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Characteristics Analysis ◽  
Tract Infections

scholarly journals Detection and Analysis of Respiratory Pathogens in Hospitalized Children with Acute Respiratory Tract Infections by a Multiplex-PCR Assay Based on the Genetic Analyzer Platform

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Pengbo Guo ◽  
Yanhong Wang ◽  
Mingjin Zhou ◽  
Shiyue Mei ◽  
Yibing Cheng
Keyword(s):  
Respiratory Tract ◽  
Multiplex Pcr ◽  
Respiratory Tract Infections ◽  
Hospitalized Children ◽  
Respiratory Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Positive Rate ◽  
Tract Infections ◽  
Genetic Analyzer

Background: Despite the considerable incidence of acute respiratory tract infections (ARTI) among children in Henan province, detailed epidemiological information is limited Objectives: . Following a retrospective design, the current study aimed to analyze the epidemiological trends of respiratory pathogens in children hospitalized at Henan Children’s Hospital. Methods: A total of 11306 children (age range, 4 days to 15 years) diagnosed with ARTI admitted to Henan Children’s Hospital from March 1, 2019, to February 29, 2020, were enrolled. Nasopharyngeal swabs, alveolar lavage fluid, or sputum samples were analyzed for the presence of 12 pathogens via a multiplex-PCR assay based on the Genetic Analyzer platform. Data of 11306 samples were eligible for analysis. Results: The total positive rate was 78.1% (8831/11306). Of 8831 positive samples, 7017 (79.5%, 7017/8831) had a single pathogen and 1814 (20.5%, 1814/11306) had multiple pathogens. Human rhinovirus was the most common pathogen (25.4%, 2874/11306), followed by mycoplasma (18.1%, 2050/11306) and human respiratory syncytial virus (15.8%, 1783/11306). There was no significant difference concerning the positive rate of respiratory pathogens between boys and girls (χ2 = 0.286, P = 0.593). Children were more likely to be infected in autumn and winter than in spring and summer (9722 vs. 1584, respectively). Conclusions: Human rhinovirus and mycoplasma were the most commonly detected pathogens. The positive rate of chlamydia was independent of the season, while positive rates of other pathogens were season-related. The positive rate of influenza A (H1N1) was independent of age, while for other pathogens, it was age-dependent. This study demonstrated species-level information on the pathogens, which can improve the prevention and treatment of hospitalized children with ARTI.

scholarly journals Impact of COVID-19 pandemic on the prevalence of respiratory viruses in children with lower respiratory tract infections in China

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Pengcheng Liu ◽  
Menghua Xu ◽  
Lingfeng Cao ◽  
Liyun Su ◽  
Lijuan Lu ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Respiratory Viruses ◽  
Hospitalized Children ◽  
Lower Respiratory Tract Infections ◽  
Syncytial Virus ◽  
Tract Infections ◽  
Respiratory Specimens

Abstract Background The multifaceted non-pharmaceutical interventions (NPIs) taken during the COVID-19 pandemic not only decrease the spreading of the SARS-CoV-2, but have impact on the prevalence of other viruses. This study aimed to explore the prevalence of common respiratory viruses among hospitalized children with lower respiratory tract infections (LRTI) in China during the COVID-19 pandemic. Methods Respiratory specimens were obtained from children with LRTI at Children’s Hospital of Fudan University for detection of respiratory syncytial virus (RSV), adenovirus (ADV), parainfluenza virus (PIV) 1 to 3, influenza virus A (FluA), influenza virus B (FluB), human metapneumovirus (MPV) and rhinovirus (RV). The data were analyzed and compared between the year of 2020 (COVID-19 pandemic) and 2019 (before COVID-19 pandemic). Results A total of 7107 patients were enrolled, including 4600 patients in 2019 and 2507 patients in 2020. Compared with 2019, we observed an unprecedented reduction of RSV, ADV, FluA, FluB, and MPV infections in 2020, despite of reopening of schools in June, 2020. However, the RV infection was significantly increased in 2020 and a sharp increase was observed especially after reopening of schools. Besides, the PIV infection showed resurgent characteristic after September of 2020. The mixed infections were significantly less frequent in 2020 compared with the year of 2019. Conclusions The NPIs during the COVID-19 pandemic have great impact on the prevalence of common respiratory viruses in China. Meanwhile, we do need to be cautious of a possible resurgence of some respiratory viruses as the COVID-19 restrictions are relaxed.

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