scholarly journals Lack of association of interleukin-6 and interleukin-8 gene polymorphisms in Chinese patients with systemic lupus erythematosus

2006 ◽  
Vol 20 (6) ◽  
pp. 255-259 ◽  
Author(s):  
Chung-Ming Huang ◽  
An-Ping Huo ◽  
Chang-Hai Tsai ◽  
Chi-Lan Chen ◽  
Fuu-Jen Tsai
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Interleukin 6 ◽  
Gene Polymorphisms ◽  
Interleukin 8 ◽  
Chinese Patients ◽  
Systemic Lupus

Interleukin 4 and STAT6 gene polymorphisms are associated with systemic lupus erythematosus in Chinese patients

Lupus ◽  
2010 ◽  
Vol 19 (10) ◽  
pp. 1219-1228 ◽  
Author(s):  
H-H. Yu ◽  
P-H. Liu ◽  
Y-C. Lin ◽  
WJ Chen ◽  
J-H. Lee ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Gene Polymorphisms ◽  
Chinese Patients ◽  
Interleukin 4 ◽  
Systemic Lupus

scholarly journals Interleukin 6 Gene Polymorphisms Are Associated with Systemic Lupus Erythematosus in Koreans

2010 ◽  
Vol 37 (11) ◽  
pp. 2251-2258 ◽  
Author(s):  
JA-YOUNG JEON ◽  
HYOUN-AH KIM ◽  
SEUNG-HYUN KIM ◽  
HAE-SIM PARK ◽  
CHANG-HEE SUH
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Hela Cells ◽  
Lupus Erythematosus ◽  
Interleukin 6 ◽  
Gene Polymorphisms ◽  
Promoter Activity ◽  
Luciferase Reporter ◽  
Systemic Lupus ◽  
Exon 2 ◽  
Hep3b Cells

Objective.Interleukin 6 (IL-6) gene polymorphisms are known to play a role in chronic inflammatory disorders. We searched for polymorphisms in theIL-6gene and described their pathogenic role in Korean patients with systemic lupus erythematosus (SLE).Methods.Genomic DNA was extracted from 151 patients with SLE and 151 controls, and about 1.4 kb-sizedIL-6genes located between promoter region and exon 2 region were amplified by polymerase chain reaction. The promoter activity was analyzed by luciferase reporter assay in Hep3B cells and HeLa cells.Results.We identified 4 single-nucleotide polymorphisms (SNP; −572 C > G, −278 A > C in the promoter, and 330 T > G, and 334 A > T in exon 2) and a −373 AnTntract polymorphism in theIL-6gene. The genotype frequency, −373 A10T11, −278 C, and 334 T allele were significantly associated with SLE (p < 0.001, p = 0.03 and p = 0.005, respectively). Patients with SLE carrying the −572 G allele had anti-dsDNA more frequently (p = 0.007). In addition, thrombocytopenia was significantly more common in patients carrying the −278 C allele (p = 0.006). In the haplotype analysis, patients with SLE had more frequently haplotype HT3 (CA10T11ATA, dominant model, p = 0.012) that was associated with arthritis, leukopenia, anti-dsDNA, and hypocomplementemia. Promoter reporter structures carrying the −278 C allele displayed significantly higher promoter activity than the −278 A allele in Hep3B cells (p < 0.001) and HeLa cells (p < 0.001).Conclusion.These data suggest thatIL-6gene polymorphisms are associated with disease susceptibility and phenotype of SLE. In addition, promoter polymorphisms may be involved in regulation ofIL-6expression.

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