Simultaneous Separation of Four Types of Steroid Hormones by Micellar Electrokinetic Chromatography with Cetyltrimethylammonium Bromide

2008 ◽  
Vol 55 (3) ◽  
pp. 594-605 ◽  
Author(s):  
Lin-Chau Chang ◽  
Tai-Chia Chiu ◽  
Shoei-Sheng Lee ◽  
Ching-Hua Kuo ◽  
Tien-Chun Chang
Keyword(s):  
Steroid Hormones ◽  
Cetyltrimethylammonium Bromide ◽  
Micellar Electrokinetic Chromatography ◽  
Electrokinetic Chromatography ◽  
Simultaneous Separation

scholarly journals Biomedical Evaluation of Cortisol, Cortisone, and Corticosterone along with Testosterone and Epitestosterone Applying Micellar Electrokinetic Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Tomasz Bączek ◽  
Ilona Olędzka ◽  
Lucyna Konieczna ◽  
Piotr Kowalski ◽  
Alina Plenis
Keyword(s):  
Steroid Hormones ◽  
Human Urine ◽  
Micellar Electrokinetic Chromatography ◽  
Solid Phase ◽  
Urine Samples ◽  
Routine Practice ◽  
Electrokinetic Chromatography ◽  
Human Urine Samples ◽  
Low Concentrations ◽  
Speed Accuracy

The validated micellar electrokinetic chromatography (MEKC) was proposed for the determination of five steroid hormones in human urine samples. That technique allowed for the separation and quantification of cortisol, cortisone, corticosterone, testosterone, and epitestosterone and was sensitive enough to detect low concentrations of these searched steroids in urine samples at the range of 2–300 ng/mL. The proposed MEKC technique with solid-phase extraction (SPE) procedure was simple, rapid, and has been successfully applied as a routine procedure to analyze steroids in human urine samples. The MEKC method offered a potential in clinical routine practice because of the short analysis time (8 min), low costs, and simultaneous analysis of five endogenous hormones. Due to its simplicity, speed, accuracy, and high recovery, the proposed method could offer a tool to determine steroid hormones as potential biomarkers in biomedical investigations, what was additionally revealed with healthy volunteers.

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