QM/MM study of the taxadiene synthase mechanism

2019 ◽  
Vol 40 (21) ◽  
pp. 1902-1910 ◽  
Author(s):  
Jeaphianne P. M. van Rijn ◽  
Andrés M. Escorcia ◽  
Walter Thiel
Keyword(s):  
Taxadiene Synthase

scholarly journals Cloning of taxadiene synthase gene into Arabidopsis thaliana (ecotype Columbia-0)

2010 ◽  
Vol 9 (12) ◽  
pp. 1734-1740 ◽  
Author(s):  
Khani Sajjad ◽  
Mehdi Sohani Mohammad ◽  
Mahna Nasser ◽  
Barar Jaleh ◽  
Saeed Hejazi Mohammad ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Taxadiene Synthase

scholarly journals Enhanced production of taxadiene in Saccharomyces cerevisiae

2020 ◽  
Author(s):  
Behnaz Nowrouzi ◽  
Rachel Li ◽  
Laura E. Walls ◽  
Leopold d’Espaux ◽  
Koray Malci ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Shake Flask ◽  
Scale Up ◽  
Cost Effective ◽  
Cancer Drug ◽  
List Type ◽  
Taxadiene Synthase ◽  
Enhanced Production ◽  
Anti Cancer ◽  
Positive Effect

AbstractCost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubility in S. cerevisiae was revealed, and the strains were strategically engineered to relieve this bottleneck. Multi-copy chromosomal integration of TASY harbouring a selection of fusion solubility tags improved taxadiene titres 22-fold, up to 57 ± 3 mg/L at 30 °C at shake flask scale. The scalability of the process was highlighted through achieving similar titres during scale up to 25 mL and 250 mL in shake flask and bioreactor cultivations, respectively. Maximum taxadiene titres of 129 ± 15 mg/L and 119 mg/L were achieved through shake flask and bioreactor cultivation, respectively, of the optimal strain at a reduced temperature of 20 °C. The results highlight the positive effect of coupling molecular biology tools with bioprocess variable optimisation on synthetic pathway development.HighlightsMaximum taxadiene titre of 129 ± 15 mg/L in Saccharomyces cerevisiae at 20 °CIntegrating fusion protein tagged-taxadiene synthase improved taxadiene titre.Consistent taxadiene titres were achieved at the micro-and mini-bioreactor scales.

Taxadiene Synthase-Catalyzed Cyclization of 6-Fluorogeranylgeranyl Diphosphate to 7-Fluoroverticillenes

2005 ◽  
Vol 127 (21) ◽  
pp. 7834-7842 ◽  
Author(s):  
Yinghua Jin ◽  
David C. Williams ◽  
Rodney Croteau ◽  
Robert M. Coates
Keyword(s):  
Taxadiene Synthase

scholarly journals Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

BMB Reports ◽  
2012 ◽  
Vol 45 (10) ◽  
pp. 589-594 ◽  
Author(s):  
Mi-Jeong Cha ◽  
Sang-Hee Shim ◽  
Sung-Hong Kim ◽  
Ok-Tae Kim ◽  
Se-Weon Lee ◽  
...  
Keyword(s):  
Taxadiene Synthase

scholarly journals Characterization and Expression Profile Analysis of a New cDNA Encoding Taxadiene Synthase from Taxus media

BMB Reports ◽  
2005 ◽  
Vol 38 (6) ◽  
pp. 668-675 ◽  
Author(s):  
Guoyin Kai ◽  
Lingxia Zhao ◽  
Lei Zhang ◽  
Zhugang Li ◽  
Binhui Guo ◽  
...  
Keyword(s):  
Expression Profile ◽  
Profile Analysis ◽  
Taxadiene Synthase ◽  
Taxus Media ◽  
Expression Profile Analysis

Taxol Production and Taxadiene Synthase Activity inTaxus canadensisCell Suspension Cultures

1997 ◽  
Vol 337 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Mehri Hezari ◽  
Raymond E.B. Ketchum ◽  
Donna M. Gibson ◽  
Rodney Croteau
Keyword(s):  
Suspension Cultures ◽  
Taxadiene Synthase ◽  
Taxol Production
Sign in / Sign up

Export Citation Format

Share Document