Polyvinyl alcohol modified polyvinylidene fluoride‐graphene oxide scaffold promotes osteogenic differentiation potential of human induced pluripotent stem cells

2020 ◽  
Vol 121 (5-6) ◽  
pp. 3185-3196 ◽  
Author(s):  
Esmaeel Azadian ◽  
Bahar Arjmand ◽  
Abdolreza Ardeshirylajimi ◽  
Simzar Hosseinzadeh ◽  
Meisam Omidi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Polyvinylidene Fluoride ◽  
Differentiation Potential ◽  
Induced Pluripotent

In vitro osteogenic differentiation potential of the human induced pluripotent stem cells augments when grown on Graphene oxide-modified nanofibers

Gene ◽  
2019 ◽  
Vol 696 ◽  
pp. 72-79 ◽  
Author(s):  
Ehsan Saburi ◽  
Maryam Islami ◽  
Simzar Hosseinzadeh ◽  
Abbas Shapouri Moghadam ◽  
Reyhaneh Nassiri Mansour ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Differentiation Potential ◽  
Induced Pluripotent

Comparison of osteogenic differentiation potential of induced pluripotent stem cells on 2D and 3D polyvinylidene fluoride scaffolds

2019 ◽  
Vol 234 (10) ◽  
pp. 17854-17862 ◽  
Author(s):  
Ali Mirzaei ◽  
Abbas Shapouri Moghadam ◽  
Mohamad Foad Abazari ◽  
Fatemeh Nejati ◽  
Sepehr Torabinejad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Polyvinylidene Fluoride ◽  
Differentiation Potential ◽  
Induced Pluripotent ◽  
2D And 3D

Concise review; the recent methods that enhance the osteogenic differentiation of human induced pluripotent stem cells

2021 ◽  
Vol 16 ◽  
Author(s):  
Yongchun Hou ◽  
Zi Yan ◽  
Zhongqi Wu
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Bone Defects ◽  
Rapid Expansion ◽  
Differentiation Potential ◽  
Skeletal Defects ◽  
Induced Pluripotent

Bone regeneration is a critical problem in modern clinical practice. Osteocytes are the most abundant cell population of mature adult bone that plays major roles in the regulation of bone formation. In humans, the segmental bone defects cannot be repaired by endogenous regenerative mechanisms. Bone tissue engineering (BTE) is a promising option for the treatment of difficult segmental and skeletal defects. BTE requires suitable cell sources with rapid expansion and adequate function, inducible factors, and scaffolds, to successfully regenerate or repair the bone injury. To overcome the disadvantages of using allogeneic and autologous tissue grafts, stem cell-based therapy has progressed an advanced topic in regenerative medicine. In the past few decades, numerous attempts have been made to generate osteocytes by using pluripotent stem cells (PSCs) for repair and regeneration of bone defects. Human induced pluripotent stem cells (hiPSCs) are PSCs that can self-renew and differentiate into a variety of cell types. Reprogramming of human somatic cells into hiPSCs provides a new opportunity for regenerative medicine, cell-based drug discovery, disease modeling, and toxicity assessment. The ability to differentiate hiPSCs towards mesenchymal stem cells (iPSC-MSCs) is essential for treating bone-related damages and injuries. Several in vitro studies revealed that the cell type of origin for iPSCs, a combination of transcription factors, the type of promoter in the vector, transduction methods, scaffolds, differentiating techniques, and culture medium may affect the osteogenic differentiation potential of hiPSCs. This review will focus on several factors that influence the osteogenic differentiation of human iPSCs.

scholarly journals Collagen coated electrospun polyethersulfon nanofibers improved insulin producing cells differentiation potential of human induced pluripotent stem cells

2018 ◽  
Vol 46 (sup3) ◽  
pp. S734-S739 ◽  
Author(s):  
Reyhaneh Nassiri Mansour ◽  
Fatemeh Soleimanifar ◽  
Mohamad Foad Abazari ◽  
Sepehr Torabinejad ◽  
Abdolreza Ardeshirylajimi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Differentiation Potential ◽  
Insulin Producing Cells ◽  
Induced Pluripotent

scholarly journals Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells

2015 ◽  
Vol 24 (21) ◽  
pp. 2561-2576 ◽  
Author(s):  
Ping-Hsing Tsai ◽  
Yueh Chien ◽  
Jen-Hua Chuang ◽  
Shih-Jie Chou ◽  
Chian-Hsu Chien ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Mitochondrial Functions ◽  
Induced Pluripotent

283 GENERATION AND CHARACTERIZATION OF BOVINE INDUCED PLURIPOTENT STEM CELLS

2013 ◽  
Vol 25 (1) ◽  
pp. 289
Author(s):  
O. J. Koo ◽  
H. S. Kwon ◽  
D. K. Kwon ◽  
K. S. Kang ◽  
B. C. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Transgenic Animals ◽  
Mouse Embryonic Fibroblast ◽  
Embryoid Bodies ◽  
Differentiation Potential ◽  
Serum Replacement ◽  
Induced Pluripotent ◽  
Large Animals

Stem cells in large animals are an excellent model for cell therapy research and fine resources for producing transgenic animals. However, there are only few reports of stem cells in large animals because of technical differences between species. In this report, we successfully generate bovine induced pluripotent stem cells (iPSC) using 4 human reprogramming factors (Oct4, Sox2, Klf4, and c-myc) under control of PiggyBac transposition vector. Fibroblasts derived from bovine fetuses were transfected using FugeneHD agent. After 21 days, colony-shaped structures on the culture plates were mechanically detached and then seeded on a mouse embryonic fibroblast (MEF) feeder layer pretreated with mitomycin C. The culture medium was DMEM/F12 supplemented with 20% serum replacement, 5 ng mL–1 basic fibroblast growth factor (bFGF), 0.1 mM β-mercaptoethanol, 1% NEAA, and 1% penicillin-streptomycin antibiotics. The iPSC colonies showed alkaline phosphatase activity and expressed several pluripotency markers (Oct4, Sox2, SSEA1, and SSEA4). To confirm differentiation potential, the iPSC were cultured as embryoid bodies and then plated again. βIII-tubulin (ectoderm) and GFAP or α-SMA (mesoderm) were well expressed on the attached cells. The results revealed that the bovine fibroblasts were well inducted to iPSC that had potential of multilineage differentiation. We hope this technology contributes to improving transgenic cattle production. This study was financially supported by IPET (grant # 109023-05-3-CG000, 111078-03-1-CG000) and the BK21 program for Veterinary Science.

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