miRNA expression profiling uncovers a role of miR‐302b‐3p in regulating skin fibroblasts senescence

2019 ◽  
Vol 121 (1) ◽  
pp. 70-80 ◽  
Author(s):  
Jingyong Tan ◽  
Longyuan Hu ◽  
Xin Yang ◽  
Xin Zhang ◽  
Canshen Wei ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Expression Profiling ◽  
Skin Fibroblasts ◽  
Mirna Expression Profiling

scholarly journals Identification of Differentially Expressed MicroRNAs in Osteosarcoma

Sarcoma ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Rishi R. Lulla ◽  
Fabricio F. Costa ◽  
Jared M. Bischof ◽  
Pauline M. Chou ◽  
Maria de F. Bonaldo ◽  
...  
Keyword(s):  
High Throughput ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Osteosarcoma Cell ◽  
Human Osteoblasts ◽  
Normal Human ◽  
Mirna Expression Profiling

A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.

Comparative miRNA Expression Profiling of Circulating Exosomes From MGUS and Smoldering Multiple Myeloma Patients

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 3975-3975
Author(s):  
Salomon Manier ◽  
Erica N Boswell ◽  
Antonio Sacco ◽  
Patricia Maiso ◽  
Ranjit Banwait ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Advisory Board ◽  
Immunogold Labeling ◽  
Differentially Expressed ◽  
Mirna Expression Profiling ◽  
Lower Expression

Abstract Abstract 3975 Introduction. Exosomes are small vesicles (50–100 nm) of endocytic origin, which are released in the extra-cellular milieu by several cell types. It is known that cell-to-cell communication is partially mediated by exosomes. The role of exosomes has been shown in tumor progression, due to their ability to carry and transfer microRNAs (miRNAs) to the recipient cells. In this study, we sought to examine the role of circulating exosomes in modulating transition from a monoclonal gammopathy of undermined significance (MGUS) stage to a smoldering myeloma (SMM) stage. Method. Exosomes were collected from peripheral blood obtained from healthy individuals (n=4), MGUS patients (n=4) and SMM patients (n=4), using ultracentrifugation; and further studied by using electron microscopy and immunogold labeling for the detection of CD63 and CD81. miRNA profiling has been performed using nCounter miRNA expression assay (Nanostring® Technologies, Seattle WA). Bioinformatic software tools (TargetScan, MIRDB) were used to predict the target genes of identified miRNA and define their function. Results. Circulating exosomes were studied at ultrastructural level showing positivity for CD81 and CD63, as demonstrated by immunogold labeling and electron microscopy. We identified 16 miRNAs differentially expressed in circulating exosome obtained from MGUS patients compared to healthy subjects (p < 0.05): specifically, higher expression of miR-450a, -30e, -125a, -300 and lower expression of miR-185, -150, -98 were observed in MGUS- compared to heatly individual-derived circulating exosomes. Interestingly, miR-30e and -150 are important for modulation of NK cell activity by targeting perforin and c-Myb, respectively. We furthermore compared the miRNA expression profiling between MGUS and SMM circulating exosomes; and found 11 miRNAs differentially expressed (p < 0.05). Specifically, higher expression of miR-107 and lower expression of miR-28, -32, -548a, -939, -99a, -345, -125a, -587, -323b and -92a were observed in SMM- compared to MGUS-derived circulating exosomes. Among the de-regulated miRNAs, miR-99a, -345, -92a and -28 are known to act as tumor suppressors in prior publications. Moreover, predicted targets for miR-107 include genes involved in molding the bone marrow microenvironment. Indeed, miR-107 is known to decrease hypoxia-inducible factor-1 β (HIF-1β), miR-125a correlated with the expression level of matrix metalloproteinase 11 (MMP11), and vascular endothelial growth factor A (VEGF-A) and miR-548a regulates the expression of MMP2. Conclusion. These findings indicate that circulating exosomes differ between normal, MGUS and SMM patients, and could potentially be involved in modulating the host microenvironment for specific homing of clonal plasma cells to the bone marrow; thus providing a better understanding of the epigenetic changes responsible for the transition from an MGUS stage to a SMM stage. Disclosures: Ghobrial: Millennium: Advisory Board Other; Novartis: Advisory Board, Advisory Board Other.

scholarly journals Identification of novel MiRNAs and MiRNA expression profiling during grain development in indica rice

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 264 ◽  
Author(s):  
Ying Lan ◽  
Ning Su ◽  
Yi Shen ◽  
Rongzhi Zhang ◽  
Fuqing Wu ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Expression Profiling ◽  
Indica Rice ◽  
Grain Development ◽  
Mirna Expression Profiling

scholarly journals Left-Sided Colorectal Cancer Distinct in Indigenous African Patients Compared to Other Ethnic Groups in South Africa.

2021 ◽  
Author(s):  
M. McCabe ◽  
C. Penny ◽  
P. Magangane ◽  
S. Mirza ◽  
Y. Perner
Keyword(s):  
Colorectal Cancer ◽  
South Africa ◽  
Ethnic Groups ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Expression Patterns ◽  
Molecular Characteristics ◽  
Anatomical Site ◽  
Molecular Features ◽  
Mirna Expression Profiling

Abstract Introduction: A large proportion of indigenous African (IA) colorectal cancer (CRC) patients in South Africa are young (<50years), with no unique histopathological or molecular characteristics. Anatomical site as well as microsatellite instability (MSI) status have shown to be associated with different clinicopathological and molecular features. This study aimed to ascertain key histopathological and miRNA expression patterns in microsatellite stable (MSS) and low-frequency MSI (MSI-L) patients, to provide insight into the mechanism of the disease. Methods: A retrospective cohort (2011-2015) of MSS/MSI-L CRC patient samples diagnosed at Charlotte Maxeke Johannesburg Academic Hospital was analyzed. Samples were categorized by site [Right colon cancer (RCC) versus left (LCC)], ethnicity [IA versus other ethnic groups (OEG)] and MSI status (MSI-L vs MSS). T-test, Fischer’s exact and Chi-square tests were conducted. Additional miRNA expression profiling was performed on IA patient samples. Results: IA patients with LCC demonstrated an increased prevalence in males, sigmoid colon, signet-ring-cell morphology, MSI-L with BAT25/26 marker instability and advanced disease association. MiRNA expression profiling revealed unique clustering, with dysregulation of let-7 and miRNA-125. Conclusion: This study revealed distinct histopathological features for LCC, and suggests BAT25/26, miRNAs let-7a-5p and miRNA-125a/b-5p as negative prognostic markers in African CRC patients. Larger confirmatory studies are recommended.

Hepatic miRNA expression profiling in genetically modified mouse models of different atherosclerosis susceptibility

2016 ◽  
Vol 252 ◽  
pp. e206-e207
Author(s):  
S. Manzini ◽  
M. Busnelli ◽  
M. Chiara ◽  
C. Parolini ◽  
F. Dellera ◽  
...  
Keyword(s):  
Mouse Models ◽  
Mirna Expression ◽  
Expression Profiling ◽  
Genetically Modified ◽  
Mirna Expression Profiling
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