Betulinic acid induces apoptosis and inhibits metastasis of human renal carcinoma cells in vitro and in vivo

2018 ◽  
Vol 119 (10) ◽  
pp. 8611-8622 ◽  
Author(s):  
Chunming Yang ◽  
Yan Li ◽  
Liye Fu ◽  
Tao Jiang ◽  
Fandong Meng
Keyword(s):  
Betulinic Acid ◽  
Renal Carcinoma ◽  
Carcinoma Cells ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Anti-Ki-67 peptide nucleic acid affects the proliferation and apoptosis of human renal carcinoma cells in vitro

Life Sciences ◽  
2005 ◽  
Vol 76 (16) ◽  
pp. 1873-1881 ◽  
Author(s):  
Jun-Nian Zheng ◽  
Ya-Feng Sun ◽  
Dong-Sheng Pei ◽  
Jun-Jie Liu ◽  
Xiao-Qing Sun ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Renal Carcinoma ◽  
Carcinoma Cells ◽  
Ki 67 ◽  
Proliferation And Apoptosis ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Effects of low calcium levels on erythropoietin production by human renal carcinoma cells in culture

1987 ◽  
Vol 253 (6) ◽  
pp. C797-C801 ◽  
Author(s):  
K. Nagakura ◽  
M. Ueno ◽  
J. Brookins ◽  
B. S. Beckman ◽  
J. W. Fisher
Keyword(s):  
Lactate Dehydrogenase ◽  
Culture Medium ◽  
Renal Carcinoma ◽  
Extracellular Calcium ◽  
Carcinoma Cells ◽  
Cells In Culture ◽  
Low Calcium ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Recent investigations have shown that calcium entry blockers enhance the effects of hypoxia on erythropoietin (Ep) production in vivo. To determine whether deprivation of calcium increases Ep production and/or release, studies were carried out to determine the effects of low levels of extracellular calcium on Ep (radioimmunoassay) secretion in human renal carcinoma cells in culture. Low extracellular calcium levels (0.3 mM) in culture medium significantly (P less than 0.01) enhanced Ep secretion (64-145% increase per day) by renal carcinoma cells in culture when compared with a concentration of 1.9 mM calcium in the control culture medium (23-68% increase per day) incubated for 24 h or more. A 53% increase per day in Ep secretion was also produced by the calmodulin inhibitor trifluoperazine. To determine whether the effects of low calcium levels on Ep production could be due to nonspecific leakage of large intracellular molecules caused by a permeabilization of the cell membrane, the effect of low calcium levels in the cultures of the renal carcinoma cells on lactate dehydrogenase release into the culture medium was studied. Low calcium concentrations failed to significantly enhance lactate dehydrogenase secretion by the renal carcinoma cells. In conclusion, our results indicate a possible involvement of the calcium ion and calmodulin in the biosynthetic pathway for Ep and that calcium may exert a suppressive effect on Ep production.

scholarly journals Interleukin 1 modulates growth of human renal carcinoma cells in vitro

1995 ◽  
Vol 71 (4) ◽  
pp. 794-800 ◽  
Author(s):  
I Koch ◽  
H Depenbrock ◽  
S Danhauser-Riedl ◽  
JW Rastetter ◽  
A-R Hanauske
Keyword(s):  
Renal Carcinoma ◽  
Interleukin 1 ◽  
Carcinoma Cells ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

scholarly journals In Vitro Antitumor Effects of AHR Ligands Aminoflavone (AFP 464) and Benzothiazole (5F 203) in Human Renal Carcinoma Cells

2017 ◽  
Vol 118 (12) ◽  
pp. 4526-4535 ◽  
Author(s):  
Gabriela A. Luzzani ◽  
Mariana A. Callero ◽  
Anchala I. Kuruppu ◽  
Valentina Trapani ◽  
Carolina Flumian ◽  
...  
Keyword(s):  
Renal Carcinoma ◽  
Carcinoma Cells ◽  
Antitumor Effects ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

In-vitro antioxidant and selective cytotoxicity of Garcinia cambogia and Garcinia indica leaf extracts on human kidney cancer cell line

2021 ◽  
Vol 12 (3) ◽  
pp. 1718-1728
Author(s):  
Varsha Jayakar ◽  
Vinayak Lokapur ◽  
Manjula Shantaram
Keyword(s):  
Kidney Cancer ◽  
Renal Carcinoma ◽  
Cancer Cell Line ◽  
Carcinoma Cells ◽  
Garcinia Cambogia ◽  
Selective Cytotoxicity ◽  
Garcinia Indica ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Kidney cancer is one among the top 10 cancers. Renal cell carcinoma (RCC) is commonly known kidney cancer. Almost all clinically targeted drugs used in treating RCC have many aftereffects. To overcome this problem with herbal products, the present study is aimed to investigate the preliminary phytochemicals, antioxidants and selective cytotoxicity of aqueous leaves extract of Garcinia cambogia (GC)and Garcinia indica  (GI)on(HEK-293) human embryonic kidney cells and (A498)human renal carcinoma cells. The phytochemical analyses were done using standard protocols. In-vitro antioxidant activity was carried out using DPPH, FRAP, and Phosphomolybdenum assay. Anticancer activity on A498 kidney cancer cell line was evaluated by MTT assay and the selectivity index was calculated. Preliminary phytochemical analysis of GC and GI leaf extract divulged the presence of various phyto-constituents. GI extract revealed higher phenolic content while flavonoid content was more in GC extract compared to alkaloids and saponins. Both plant extracts exhibited higher antioxidant capacities based on the test performed.GC and GIleaf extract wasselectively cytotoxic in-vitro to (A498) human renal carcinoma cells and can be safely used against kidney cancer at 500 µM (GC extract)and 300 µM (GI extract). Selective index for GC and GI extract was15.9 and 2.4respectively. The results indicate that GC and GI extracts are a favourable antioxidant and anti-cancer agents for A498 human renal carcinoma cells. However, further studies to isolate the bioactive compounds responsible for these activities are underway and to explore their molecular mechanism.

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