Recent Advances in CRISPR-Cas9 Genome Editing Technology for Biological and Biomedical Investigations

2017 ◽  
Vol 119 (1) ◽  
pp. 81-94 ◽  
Author(s):  
Vijai Singh ◽  
Nisarg Gohil ◽  
Robert Ramírez García ◽  
Darren Braddick ◽  
Christian Kuete Fofié
Keyword(s):  
Genome Editing ◽  
Recent Advances

Recent advances of genome editing and related technologies in China

Gene Therapy ◽  
2020 ◽  
Vol 27 (7-8) ◽  
pp. 312-320
Author(s):  
Wen Sun ◽  
Haoyi Wang
Keyword(s):  
Genome Editing ◽  
Recent Advances

Recent Advances in Therapeutic Genome Editing in China

2018 ◽  
Vol 29 (2) ◽  
pp. 136-145 ◽  
Author(s):  
Yang Yang ◽  
Qingnan Wang ◽  
Qian Li ◽  
Ke Men ◽  
Zhiyao He ◽  
...  
Keyword(s):  
Genome Editing ◽  
Recent Advances

scholarly journals Genome editing in large animals: current status and future prospects

2019 ◽  
Vol 6 (3) ◽  
pp. 402-420 ◽  
Author(s):  
Jianguo Zhao ◽  
Liangxue Lai ◽  
Weizhi Ji ◽  
Qi Zhou
Keyword(s):  
Regenerative Medicine ◽  
Genome Editing ◽  
Biomedical Research ◽  
Human Disease ◽  
Gene Editing ◽  
Disease Modeling ◽  
Current Status ◽  
Future Prospects ◽  
Recent Advances ◽  
Large Animals

AbstractLarge animals (non-human primates, livestock and dogs) are playing important roles in biomedical research, and large livestock animals serve as important sources of meat and milk. The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research. In this review, we briefly introduce the recent advances in nuclease-meditated gene editing tools, and we outline these editing tools’ applications in human disease modeling, regenerative medicine and agriculture. Additionally, we provide perspectives regarding the challenges and prospects of the new genome editing technology.

scholarly journals Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

2018 ◽  
Vol 6 (3) ◽  
pp. 438-451 ◽  
Author(s):  
Yuwei Zhu ◽  
Zhiwei Huang
Keyword(s):  
Nucleic Acids ◽  
Genome Editing ◽  
Adaptive Immunity ◽  
Structural Biology ◽  
Structural Studies ◽  
Guide Rnas ◽  
Recent Advances ◽  
Cas Proteins

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.

scholarly journals A practical guide to CRISPR/Cas9 genome editing in Lepidoptera

2017 ◽  
Author(s):  
Linlin Zhang ◽  
Robert D. Reed
Keyword(s):  
Experimental Design ◽  
Genome Editing ◽  
Model Systems ◽  
Strong Impact ◽  
Practical Advice ◽  
Entire Process ◽  
Practical Guide ◽  
Recent Advances ◽  
Detailed Protocol

AbstractCRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.

scholarly journals 231 Structure and mechanism of CRISPR/Cas9

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 56-57
Author(s):  
David W Taylor
Keyword(s):  
Human Health ◽  
Genome Editing ◽  
Genome Engineering ◽  
Structure And Function ◽  
Recent Advances ◽  
And Function

Abstract Recent advances in genome editing using CRISPR-Cas9 and related technologies have revolutionized the ability to manipulate genes in a rapid, precise, and flexible manner. These advances have spurred an explosion of interest in the possible ways in which genome editing can improve human health. I will provide an overview of CRISPR-Cas systems, the structure and function of CRISPR-Cas9, and the repurposing of CRISPR-Cas9 for genome engineering.

scholarly journals Recent advances in genome editing of stem cells for drug discovery and therapeutic application

2020 ◽  
Vol 209 ◽  
pp. 107501 ◽  
Author(s):  
Jaesuk Lee ◽  
Delger Bayarsaikhan ◽  
Govigerel Bayarsaikhan ◽  
Jin-Soo Kim ◽  
Elizabeth Schwarzbach ◽  
...  
Keyword(s):  
Stem Cells ◽  
Drug Discovery ◽  
Genome Editing ◽  
Therapeutic Application ◽  
Recent Advances

scholarly journals Recent advances of Cas12a applications in bacteria

2021 ◽  
Author(s):  
Meliawati Meliawati ◽  
Christoph Schilling ◽  
Jochen Schmid
Keyword(s):  
Genome Editing ◽  
Dna Sequences ◽  
Genome Engineering ◽  
Bacterial Genome ◽  
Adaptive Immune System ◽  
Eukaryotic Cell ◽  
Promising Alternative ◽  
Guide Rna ◽  
Recent Advances ◽  
Versatile Tool

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genome engineering and related technologies have revolutionized biotechnology over the last decade by enhancing the efficiency of sophisticated biological systems. Cas12a (Cpf1) is an RNA-guided endonuclease associated to the CRISPR adaptive immune system found in many prokaryotes. Contrary to its more prominent counterpart Cas9, Cas12a recognizes A/T rich DNA sequences and is able to process its corresponding guide RNA directly, rendering it a versatile tool for multiplex genome editing efforts and other applications in biotechnology. While Cas12a has been extensively used in eukaryotic cell systems, microbial applications are still limited. In this review, we highlight the mechanistic and functional differences between Cas12a and Cas9 and focus on recent advances of applications using Cas12a in bacterial hosts. Furthermore, we discuss advantages as well as current challenges and give a future outlook for this promising alternative CRISPR-Cas system for bacterial genome editing and beyond. Key points • Cas12a is a powerful tool for genome engineering and transcriptional perturbation • Cas12a causes less toxic side effects in bacteria than Cas9 • Self-processing of crRNA arrays facilitates multiplexing approaches

scholarly journals CRISPR Pioneers Win 2020 Nobel Prize for Chemistry

2020 ◽  
Author(s):  
Dariush D. FARHUD ◽  
Marjan ZARIF-YEGANEH
Keyword(s):  
Clinical Practice ◽  
Human Genome ◽  
Genome Editing ◽  
Nobel Prize ◽  
Gene Editing ◽  
High Accuracy ◽  
Recent Advances ◽  
Programmable Nucleases

Over the last few years, the development of genome editing has revolutionized research on the human genome. Recent advances in developing programmable nucleases, such as meganucleases, ZFNs, TALENs and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas, has greatly expedited the progress of gene editing from concept to clinical practice. The CRISPR has advantages over other nuclease-based genome editing tools due to its high accuracy, efficiency, and strong specificity. Eight years after CRISPR application for human genome edition by Emmanuelle Charpentier and Jennifer A. Doudna, the 2020 Nobel Prize in Chemistry has been jointly given to them for development of CRISPR-Cas9 gene editing, allows scientists to precisely cut and edit of DNA.

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