Morphological and biochemical characterization of mineralizing primary cultures of avian growth plate chondrocytes: Evidence for cellular processing of Ca2+ and Pi prior to matrix mineralization

1995 ◽  
Vol 57 (2) ◽  
pp. 218-237 ◽  
Author(s):  
Licia N. Y. Wu ◽  
Yoshinori Ishikawa ◽  
Glenn R. Sauer ◽  
Brian R. Genge ◽  
Fackson Mwale ◽  
...  
Keyword(s):  
Growth Plate ◽  
Biochemical Characterization ◽  
Primary Cultures ◽  
Growth Plate Chondrocytes ◽  
Matrix Mineralization ◽  
Cellular Processing

Pi transport regulation by chicken growth plate chondrocytes

1994 ◽  
Vol 267 (1) ◽  
pp. E24-E31
Author(s):  
C. Montessuit ◽  
J. P. Bonjour ◽  
J. Caverzasio
Keyword(s):  
Growth Plate ◽  
Primary Cultures ◽  
Growth Plate Chondrocytes ◽  
Phosphonoformic Acid ◽  
Pi Transport ◽  
Igf I ◽  
Sodium Dependent ◽  
Half Maximal Effective Concentration ◽  
Cartilage Cells ◽  
Extracellular Sodium

Inorganic phosphate (Pi) is a key element for the growth and mineralization of the epiphyseal cartilage. In this study, the characteristics of the transport of Pi in growth plate chondrocytes have been determined using primary cultures of chicken growth plate cartilage cells. The uptake of Pi was significantly increased in the presence of extracellular sodium. The kinetic parameters of the saturable sodium-dependent Pi transport (NaPiT) were determined. The Michaelis constant for Pi was 0.443 +/- 0.095 mM, and the concentration of sodium with which half-maximal Pi transport was observed was 48.0 +/- 8.7 mM. Stoichiometric analysis suggested that more than one sodium ion was cotransported with each Pi molecule. NaPiT was sensitive to inhibition by Pi analogues such as phosphonoformic acid and arsenate. These data strongly suggest that Pi uptake by chicken growth plate chondrocytes is a carrier-mediated process driven by the transmembrane electrochemical gradient of sodium. Two important regulators of biosynthetic activities of growth plate chondrocytes, insulin-like growth factor I (IGF-I) and parathyroid hormone (PTH), selectively regulated Pi transport. With IGF-I, maximal stimulation (117 +/- 7% above control) was observed at doses > 5 nM, with an half-maximal effective concentration of 0.46 +/- 0.18 nM. A significant effect was observed after 1 h of exposure and was maintained for up to 24 h. PTH increased Pi transport with a biphasic dose-response curve. The change in NaPiT was transient, being maximally observed after 8 h (58 +/- 8%) and unexpressed after 24 h.(ABSTRACT TRUNCATED AT 250 WORDS)

Characterization of Voltage-Sensitive Calcium Channels in Growth Plate Chondrocytes

1997 ◽  
Vol 234 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Michael J. Zuscik ◽  
Thomas E. Gunter ◽  
J.Edward Puzas ◽  
Randy N. Rosier
Keyword(s):  
Calcium Channels ◽  
Growth Plate ◽  
Growth Plate Chondrocytes

Characterization of the Mineralization Process in Cultures of Rabbit Growth Plate Chondrocytes

1993 ◽  
Vol 156 (2) ◽  
pp. 372-380 ◽  
Author(s):  
Akitoshi Jikko ◽  
Takaaki Aoba ◽  
Hiroshi Murakami ◽  
Yoshiro Takano ◽  
Masahiro Iwamoto ◽  
...  
Keyword(s):  
Growth Plate ◽  
Mineralization Process ◽  
Growth Plate Chondrocytes

scholarly journals The biochemical characterization of aggrecan from normal and tibial-dyschondroplastic chicken growth-plate cartilage

2000 ◽  
Vol 351 (2) ◽  
pp. 517-525 ◽  
Author(s):  
Chris TSELEPIS ◽  
Alvin P. L. KWAN ◽  
David THORNTON ◽  
John SHEEHAN
Keyword(s):  
Growth Plate ◽  
Endochondral Ossification ◽  
Biochemical Characterization ◽  
Marked Decrease ◽  
Bone Development ◽  
Cartilage Lesion ◽  
Keratan Sulphate ◽  
Biochemical Characteristics ◽  
Tibial Dyschondroplasia

Tibial dyschondroplasia (TD) is a disorder of endochondral ossification characterized by the presence of an avascular, non-mineralized cartilage lesion extending from the growth plate into the metaphysis. Cells within the TD growth plate fail to differentiate to full hypertrophy, and instead appear to maintain a ‘pre-hypertrophic’or ‘transitional’status. Studies of the expression and distribution of cartilage matrix macromolecules in the TD growth plate have shown a marked decrease in the levels of aggrecan in the TD matrix. In the present study we compared the biochemical characteristics of the aggrecan molecules extracted from normal epiphyseal and TD cartilage. We have shown three major differences between normal and TD cartilage aggrecan. These are: (1) increase in molecular mass; (2) increase in the number of keratan sulphate chains; and (3) difference in the pattern of sulphation in TD aggrecan. Such changes in biochemical characteristics of the aggrecan monomers in TD cartilage may be associated with the lack of mineralization of the diseased cartilage. The present study provides a basis for further investigations into the importance of proteoglycans in normal and pathological bone development.

scholarly journals Effects of Calcitonin and Parathyroid Hormone on Calcification of Primary Cultures of Chicken Growth Plate Chondrocytes

1997 ◽  
Vol 12 (3) ◽  
pp. 356-366 ◽  
Author(s):  
Yoshinori Ishikawa ◽  
Licia N. Y. Wu ◽  
Brian R. Genge ◽  
Fackson Mwale ◽  
Roy E. Wuthier
Keyword(s):  
Parathyroid Hormone ◽  
Growth Plate ◽  
Primary Cultures ◽  
Growth Plate Chondrocytes
Sign in / Sign up

Export Citation Format

Share Document