Cell cycle dependent distribution of proliferating cell nuclear antigen/cyclin and cdc2-kinase in mouse T-lymphoma cells

1993 ◽  
Vol 52 (3) ◽  
pp. 362-372 ◽  
Author(s):  
David A. Brott ◽  
James D. Alvey ◽  
Michael R. Bleavins ◽  
Felix A. de la Iglesia ◽  
Narendra D. Lalwani
Keyword(s):  
Cell Cycle ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Cdc2 Kinase ◽  
Lymphoma Cells ◽  
T Lymphoma Cells ◽  
T Lymphoma ◽  
Cycle Dependent ◽  
Proliferating Cell

scholarly journals The p27–Skp2 axis mediates glucocorticoid-induced cell cycle arrest in T-lymphoma cells

Cell Cycle ◽  
2013 ◽  
Vol 12 (16) ◽  
pp. 2625-2635 ◽  
Author(s):  
Michael K Kullmann ◽  
Claudia Grubbauer ◽  
Katrin Goetsch ◽  
Heidelinde Jäkel ◽  
Silvio R Podmirseg ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Lymphoma Cells ◽  
Cycle Arrest ◽  
T Lymphoma Cells ◽  
T Lymphoma

Demonstration of cell cycle kinetics in thyroid primary culture by immunostaining of proliferating cell nuclear antigen: differences in cyclic AMP-dependent and -independent mitogenic stimulations

1993 ◽  
Vol 105 (1) ◽  
pp. 69-80 ◽  
Author(s):  
M. Baptist ◽  
J.E. Dumont ◽  
P.P. Roger
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Primary Culture ◽  
Proliferating Cell Nuclear Antigen ◽  
Cell Cycle Progression ◽  
Nuclear Antigen ◽  
Major Influence ◽  
Cell Cycle Kinetics ◽  
Experimental Conditions ◽  
Proliferating Cell

In this study, experimental conditions are described that allowed us to follow the fate of the DNA polymerase delta-associated proliferating cell nuclear antigen (PCNA), by immunolabeling during the overall cell cycle. Differences in subcellular localization or the presence of PCNA allowed us to identify each phase of the cell cycle. Using these cell cycle markers in dog thyroid epithelial cells in primary culture, we found unexpected differences in cell cycle kinetics, in response to stimulations through cAMP-dependent and cAMP-independent pathways. These provide a new dimension to the view that the two pathways are largely separate, but co-operate on DNA synthesis initiation. More precisely, thyrotropin (TSH), acting via cAMP, exerts a potent triggering effect on DNA synthesis, associated with a precocious induction of PCNA appearance. This constitutes the major influence of TSH (cAMP) in determining cell cycle progression, which is only partly moderated by TSH-dependent lengthening of S- and G2-phases.

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